A5065075536- Plant Molecular Biology Research
- Plant Reproductive Biology
- Light effects on plants
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Plant Gene Expression Analysis
- Marine Invertebrate Physiology and Ecology
- Gene expression and cancer classification
- Marine Ecology and Invasive Species
- Molecular Biology Techniques and Applications
- Polysaccharides and Plant Cell Walls
- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- Plant and Biological Electrophysiology Studies
- Plant Virus Research Studies
- Invertebrate Immune Response Mechanisms
- Single-cell and spatial transcriptomics
- Plant Genetic and Mutation Studies
- Genomics and Chromatin Dynamics
- Plant responses to water stress
- Planarian Biology and Electrostimulation
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Gene Regulatory Network Analysis
Heidelberg University
2015-2024
Lohmann (Germany)
2005-2019
Czech Academy of Sciences, Biology Centre
2019
Max Planck Institute for Developmental Biology
2003-2010
University of Tübingen
2009
Max Planck Society
2008-2009
Friedrich Miescher Laboratory
2009
Heidelberg Institute for Stem Cell Technology and Experimental Medicine
2009
Oregon State University
2007
Salk Institute for Biological Studies
1997-2005
Flowering of Arabidopsis is regulated by several environmental and endogenous signals. An important integrator these inputs the FLOWERING LOCUS T ( FT ) gene, which encodes a small, possibly mobile protein. A primary response to floral induction activation RNA expression in leaves. Because flowers form at distant site, shoot apex, data suggest that primarily controls timing flowering. Integration temporal spatial information mediated part bZIP transcription factor FD, already expressed apex...
Building expression constructs for transgenesis is one of the fundamental day-to-day tasks in modern biology. Traditionally it based on a multitude type II restriction endonucleases and T4 DNA ligase. Especially case long inserts applications requiring high-throughput, this approach limited by number available unique sites need designing individual cloning strategies each project. Several alternative systems have been developed recent years to overcome these issues, including IIS enzyme...
Flowering of the reference plant Arabidopsis thaliana is controlled by several signaling pathways, which converge on a small set genes that function as pathway integrators. We have analyzed genomic response to one type floral inductive signal, photoperiod, dissect transducing this stimulus, including CONSTANS, thought be major output photoperiod pathway. Comparing effects CONSTANS with those FLOWERING LOCUS T, integrates inputs from and other we find expression profiles shoot apices plants...
The processing of Arabidopsis thaliana microRNAs (miRNAs) from longer primary transcripts (pri-miRNAs) requires the activity several proteins, including DICER-LIKE1 (DCL1), double-stranded RNA-binding protein HYPONASTIC LEAVES1 (HYL1), and zinc finger SERRATE (SE). It has been noted before that morphological appearance weak se mutants is reminiscent plants with mutations in ABH1/CBP80 CBP20, which encode two subunits nuclear cap-binding complex. We report that, like SE, complex necessary for...
Significance Cell–cell communication is a prerequisite of multicellular development and noncell autonomous stem cell induction has been conserved during evolution. Cytoplasmic bridges, called plasmodesmata, which facilitate the exchange molecules between neighboring cells, are striking innovation for cell–cell signaling in plants. Here, we show that plasmodesmata function required activity shoot apical cells Arabidopsis provide evidence inducing transcription factor WUSCHEL moves from niche...
Quantitative analysis of plant and animal morphogenesis requires accurate segmentation individual cells in volumetric images growing organs. In the last years, deep learning has provided robust automated algorithms that approach human performance, with applications to bio-image now starting emerge. Here, we present PlantSeg, a pipeline for tissues into cells. PlantSeg employs convolutional neural network predict cell boundaries graph partitioning segment based on predictions. was trained...
A major feature of embryogenesis is the specification stem cell systems, but in contrast to situation most animals, plant cells remain quiescent until postembryonic phase development. Here, we dissect how light and metabolic signals are integrated overcome dormancy at shoot apical meristem. We show on one hand that able activate expression inducer WUSCHEL independently photosynthesis this likely involves inter-regional cytokinin signaling. Metabolic signals, other hand, transduced meristem...
To maintain the balance between long-term stem cell self-renewal and differentiation, dynamic signals need to be translated into spatially precise temporally stable gene expression states. In apical plant system, local accumulation of small, highly mobile phytohormone auxin triggers differentiation while at same time, pluripotent cells are maintained throughout entire life-cycle. We find that resistant mediated but require low levels signaling for their maintenance. demonstrate WUSCHEL...
Abstract Single-cell and single-nucleus RNA-sequencing technologies capture the expression of plant genes at an unprecedented resolution. Therefore, these are gaining traction in molecular developmental biology for elucidating transcriptional changes across cell types a specific tissue or organ, upon treatments, response to biotic abiotic stresses, between genotypes. Despite rapidly accelerating use technologies, collective standardized experimental analytical procedures support acquisition...
Understanding the evolution of development in large part relies on study phylogenetically old organisms. Cnidarians, such as Hydra, have become attractive model organisms for these studies. However, despite long-term efforts, stably transgenic animals could not be generated, severely limiting functional analysis genes. Here we report efficient generation Hydra lines by embryo microinjection. One expressing EGFP revealed remarkably high motility individual endodermal epithelial cells during...
Abstract To maintain proper meristem function, cell division and differentiation must be coordinately regulated in distinct subdomains of the meristem. Although a number regulators necessary for correct organization shoot apical (SAM) have been identified, it is still largely unknown how their function integrated with cycle machinery to translate domain identity into cellular behavior. We show here that cyclin-dependent kinases CDKB2;1 CDKB2;2 are required both normal progression...
Abstract Spatial organization of signalling events the phytohormone auxin is fundamental for maintaining a dynamic transition from plant stem cells to differentiated descendants. The cambium, cell niche mediating wood formation, fundamentally depends on but its exact role and spatial obscure. Here we show that, while levels increase in differentiating cambium descendants, moderate level cambial essential activity. We identify auxin-dependent transcription factor ARF5/MONOPTEROS...