A5010509017- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Remote Sensing in Agriculture
- Remote Sensing and LiDAR Applications
- Genetic Mapping and Diversity in Plants and Animals
- Leaf Properties and Growth Measurement
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant-Microbe Interactions and Immunity
- Calcium signaling and nucleotide metabolism
- Plant Growth Enhancement Techniques
- Plant Surface Properties and Treatments
- Light effects on plants
- Fungal Biology and Applications
- Plant Pathogens and Fungal Diseases
- Plant tissue culture and regeneration
- Seed and Plant Biochemistry
- Adenosine and Purinergic Signaling
- Image Processing and 3D Reconstruction
- Seed Germination and Physiology
- Biochemical and Molecular Research
- Ecosystem dynamics and resilience
- Genetic and Environmental Crop Studies
- Polyamine Metabolism and Applications
- Plant responses to elevated CO2
Palacký University Olomouc
2015-2025
Czech Academy of Sciences, Institute of Experimental Botany
2015-2021
Agrobiotechnology Institute
2016
Gobierno de Navarra
2016
Consejo Superior de Investigaciones Científicas
2016
University Hospital Olomouc
2015
Our phenotyping and hormonal study has been aimed to characterize the role of cytokinins (CK) in drought recovery responses Arabidopsis thaliana. CK down-regulation was achieved by overexpression gene for deactivating enzyme cytokinin oxidase/dehydrogenase (CKX): constitutive (35S:CKX) or at stress onset using a dexamethasone-inducible pOp/LhGR promoter (DEX:CKX). The 35S:CKX plants exhibited slow ontogenesis higher expression levels stress-associated genes, e.g. AtP5CS1, already control...
Abstract It is known that volatile emissions from some beneficial rhizosphere microorganisms promote plant growth. Here we show compounds (VCs) emitted by phylogenetically diverse and non‐rhizhosphere bacteria fungi (including pathogens microbes do not normally interact mutualistically with plants) growth flowering of various species, including crops. In Arabidopsis plants exposed to VCs the phytopathogen Alternaria alternata , changes included enhancement photosynthesis accumulation high...
Recently emerging approaches to high-throughput plant phenotyping have discovered their importance as tools in unravelling the complex questions of growth, development and response environment, both basic applied science. High-throughput methods been also used study responses various types biotic abiotic stresses (drought, heat, salinity, nutrient-starving, UV light) but only rarely cold tolerance.
Environmental stresses have a significant effect on agricultural crop productivity worldwide. Exposure of seeds to abiotic stresses, such as salinity among others, results in lower seed viability, reduced germination, and poor seedling establishment. Alternative agronomic practices, e.g., the use plant biostimulants, attracted considerable interest from scientific community commercial enterprises. Biostimulants, i.e., products biological origin (including bacteria, fungi, seaweeds, higher...
High-throughput plant phenotyping platforms provide new possibilities for automated, fast scoring of several growth and development traits, followed over time using non-invasive sensors. Using Arabidopsis as a model offers important advantages high-throughput screening with the opportunity to extrapolate results obtained other crops commercial interest. In this study we describe highly reproducible in vitro bioassay established our OloPhen platform, suitable analysis rosette multi-well...
Abstract Adenosine undergoes ATP-dependent phosphorylation catalyzed by adenosine kinase (ADK). In plants, ADK also phosphorylates cytokinin ribosides, transport forms of the hormone. Here, we investigated substrate preferences, oligomeric states and structures ADKs from moss (Physcomitrella patens) maize (Zea mays) alongside metabolomic phenotypic analyses. We showed that dexamethasone-inducible ZmADK overexpressor lines in Arabidopsis can benefit a higher number lateral roots larger root...
Volatile compounds (VCs) emitted by phylogenetically diverse microorganisms (including plant pathogens and microbes that do not normally interact mutualistically with plants) promote photosynthesis, growth, the accumulation of high levels starch in leaves through cytokinin (CK)-regulated processes. In Arabidopsis (Arabidopsis thaliana) plants exposed to VCs, plastidic phosphoglucose isomerase (pPGI) acts as an important determinant photosynthesis likely a consequence its involvement...
Phosphoglucose isomerase (PGI) catalyzes the reversible isomerization of glucose-6-phosphate and fructose-6-phosphate. It is involved in glycolysis regeneration glucose-6-P molecules oxidative pentose phosphate pathway (OPPP). In chloroplasts illuminated mesophyll cells PGI also connects Calvin-Benson cycle with starch biosynthetic pathway. this work we isolated pgi1-3, a mutant totally lacking pPGI activity as consequence aberrant intron splicing encoding gene, PGI1. Starch content pgi1-3...
Microorganisms produce volatile compounds (VCs) that promote plant growth and photosynthesis through complex mechanisms involving cytokinin (CK) abscisic acid (ABA). We hypothesized plants' responses to microbial VCs involve posttranslational modifications of the thiol redox proteome action plastidial NADPH-dependent thioredoxin reductase C (NTRC), which regulates chloroplast status via its functional relationship with 2-Cys peroxiredoxins. To test this hypothesis, we analysed developmental,...
Three cytokinin derivatives (CKd) designated as RR-G, RR-O, and RR-V applied by foliar spraying at tillering, one compound previously described a antagonist (CKa) RR-P seed coating were tested in winter wheat spring barley field trial experiments. The aim of the study was to examine influence compounds that on number productive tillers, grain yield, endogenous CK content. With exception RR-V, measured parameters clearly showed stimulatory effects CKd tillering yield wheat. effect tillers...
Dark-induced growth (skotomorphogenesis) is primarily characterized by rapid elongation of the hypocotyl. We have studied role abscisic acid (ABA) during development young tomato (Solanum lycopersicum L.) seedlings. observed that ABA deficiency caused a reduction in hypocotyl at level cell and ABA-deficient plants could be improved treatment with exogenous ABA, through which show concentration dependent response. In addition, accumulated dark-grown seedlings grew rapidly, whereas grown under...
Plant phenotyping platforms offer automated, fast scoring of traits that simplify the selection varieties are more competitive under stress conditions. However, indoor methods frequently based on analysis plant growth in individual pots. We present a reproducible method for screening young barley populations water conditions and after subsequent rewatering. The is simple read-out data using RGB imaging, projected canopy height, as useful feature indirectly following kinetics loss population...
The role of abscisic acid (ABA) during early development was investigated in tomato seedlings. endogenous content ABA particular organs analyzed seedlings grown the dark and under blue light. Our results showed that dark-grown seedlings, accumulation maximal cotyledons elongation zone hypocotyl, whereas blue-light, distinctly reduced. data are consistent with conclusion promotes growth etiolated suggest plays an inhibitory de-etiolation photomorphogenesis tomato.
Abstract Background Plants, like all living organisms, metamorphose their bodies during lifetime. All the developmental and growth events in a plant’s life are connected to specific points time, be it seed germination, seedling emergence, appearance of first leaf, heading, flowering, fruit ripening, wilting, or death. The onset automated phenotyping methods has brought an explosion such time-to-event data. Unfortunately, not been matched by adequate data analysis methods. Results discussion...
Peas (Pisum sativum L.) belong among the world’s oldest domesticated crops, serving as a source of proteins, complex carbohydrates, vitamins and minerals. Autumn sowing allows higher biomass production well avoidance drought heat stresses late spring. However, character European continental winters limits plant growth development through cold stress. This work sought parameters that reflect tolerance pea plants consequently to suggest an afila-type cultivar with resilience winters. For this...
Repeated measurements of crop height to observe plant growth dynamics in real field conditions represent a challenging task. Although there are ways collect data using sensors on UAV systems, proper processing and analysis the key reliable results. As is need for specialized software solutions agricultural research breeding purposes, we present here fast algorithm ALFA LiDAR derived point-clouds extract information at many individual cereal field-plots multiple time points. Seven scanning...
Adenosine undergoes ATP-dependent phosphorylation catalyzed by adenosine kinase (ADK). In plants, ADK also phosphorylates cytokinin ribosides, transport forms of the hormone. Here, we investigated substrate preferences, oligomeric states and structures ADKs from moss (Physcomitrella patens) maize (Zea mays) alongside metabolomic phenotypic analyses. We showed that dexamethasone-inducible ZmADK overexpressor lines in Arabidopsis can benefit a higher number lateral roots larger root areas...
The estimation of plant growth is a challenging but key issue that may help us to understand crop vs. environment interactions. To perform precise and high-throughput analysis in field conditions, remote sensing using LiDAR unmanned aerial vehicles (UAV) has been developed, addition other approaches. Although there are software tools for the processing data general, no specialized automatic extraction experimental blocks with crops represent specific “points interest”. Our tool aims detect...