Summary Genetic approaches using Arabidopsis thaliana aimed at the identification of mutations affecting events involved in auxin signalling have usually led to isolation auxin‐resistant mutants. From a selection screen specifically developed isolate auxin‐hypersensitive mutants, one mutant line was selected for its increased sensitivity (× 2 3) root elongation response. The genetic analysis sax1 (hypersensitive abscisic acid and auxin) indicated that phenotype segregates as single recessive...

Polyamines are ubiquitously present in all living organisms. In plants, together with phytohormone ethylene, their metabolism plays a crucial role plant stress and ontogenesis. We have evaluated differences the responses of model plants Arabidopsis thaliana Solanum lycopersicum to abiotic stresses metabolic modulators based on key metabolite levels. Previous approaches often focused separately either polyamines, amino acids, or ethylene precursors. As these pathways directly interconnected,...

The 7B-1 tomato line (Solanum lycopersicum cv. Rutgers) is a photoperiod-sensitive male-sterile mutant, with potential application in hybrid seed production. Small RNAs (sRNAs) have been mainly characterized fruit development and ripening, but none studied respect to flower regulation of male-sterility. Using sRNA sequencing, we identified miRNAs that are potentially involved anther male-sterility mutant. Two libraries from wild type (WT) anthers were sequenced thirty two families known 23...

Nitric oxide (NO) is involved in the signalling and regulation of plant growth development responses to biotic abiotic stresses. The photoperiod-sensitive mutant 7B-1 tomato (Solanum lycopersicum) showing abscisic acid (ABA) overproduction blue light (BL)-specific tolerance osmotic stress represents a valuable model study interaction between light, hormones signalling. role NO as regulator seed germination ABA-dependent was explored wild-type under white (WL) BL. Germination data were...

We reported earlier that 7B-1 mutant in tomato (Solanum lycopersicum L., cv. Rutgers), an ABA overproducer, is defective blue light (B) signaling leading to B-specific resistance abiotic and biotic stresses. Using a methylation-sensitive amplified polymorphism (MSAP) assay, number of genes were identified, which differentially methylated between its wild type (WT) seedlings white (W), (B), red (R) lights dark (D) or response exogenous mannitol-induced The genomic methylation level was almost...

Dark-induced growth (skotomorphogenesis) is primarily characterized by rapid elongation of the hypocotyl. We have studied role abscisic acid (ABA) during development young tomato (Solanum lycopersicum L.) seedlings. observed that ABA deficiency caused a reduction in hypocotyl at level cell and ABA-deficient plants could be improved treatment with exogenous ABA, through which show concentration dependent response. In addition, accumulated dark-grown seedlings grew rapidly, whereas grown under...

A recessive single gene mutant, 7B‐1, in tomato was originally selected for its photoperiod‐dependent male sterility. The 7B‐1 mutant also has some pleiotropic effects including reduced light‐induced inhibition, i.e. de‐etiolation, of the hypocotyl long days (LD), increased seed size and weight, transpiration rate. These traits led us to investigate sensitivity exogenous hormones interaction these responses with daylength. In LD, but not short (SD), more sensitive than wild‐type (WT)...

The 7B-1 tomato (Solanum lycopersicum L. cv Rutgers) is a male-sterile mutant with enhanced tolerance to abiotic stress, which makes it potential candidate for hybrid seed breeding and stress engineering. To underline the molecular mechanism regulating male-sterility in 7B-1, transcriptomic profiles of wild type (WT) anthers were studied using mRNA sequencing (RNA-Seq). In total, 768 differentially expressed genes (DEGs) identified, including 132 up-regulated 636 down-regulated transcripts....

The role of abscisic acid (ABA) during early development was investigated in tomato seedlings. endogenous content ABA particular organs analyzed seedlings grown the dark and under blue light. Our results showed that dark-grown seedlings, accumulation maximal cotyledons elongation zone hypocotyl, whereas blue-light, distinctly reduced. data are consistent with conclusion promotes growth etiolated suggest plays an inhibitory de-etiolation photomorphogenesis tomato.

The 7B-1 tomato (Solanum lycopersicum L. 'Rutgers') is a male-sterile mutant with enhanced tolerance to abiotic stress in blue-light (BL) specific manner compared its wild-type (WT). This makes the potential candidate for hybrid seed breeding and engineering. To identify small RNAs (sRNAs) linked of 7B-1, two sRNA libraries from BL-grown WT seedlings treated simultaneously abscisic acid (ABA) mannitol were sequenced, profiles compared. Twenty nine families known microRNAs (miRNAs) 27...

Modern corn (Zea mays L.) varieties have been selected for their ability to maintain productivity in dense plantings. We tested the possibility that physiological consequence of selection involves changes responsiveness light and auxin. Etiolated seedlings two older hybrids 307 3306 elongated significantly more than a modern hybrid 3394. The level endogenous auxin activity PAT 3394 were similar. Hybrid shows resistance auxin- light-induced responses at seedling, cell, molecular levels....

The spontaneous mutant 7B-1 in tomato (Solanum lycopersicum=Lycopersicon esculentum) is a photoperiod-dependent male-sterile previously reported as resistant to various abiotic stresses specifically under blue light. Since this finding improved the potential of 7B-1's use breeding programmes, its susceptibility stress induced by coronatine (COR), phytotoxine produced several Pseudomonas syringae strains, was assessed study. found be less sensitive than corresponding wild type (WT) COR...

Light is one of the most important factor influencing plant growth and development all through their life cycle. One well-known light-regulated processes de-etiolation, i.e. switch from skotomorphogenesis to photomorphogenesis. The hormones cytokinins (CKs) play an role during establishment photomorphogenesis as exogenous CKs induced dark-grown seedlings. Most studies are conducted on model Arabidopsis, but no or few information available for crop species, such tomato (Solanum lycopersicum...

In maize, at least five auxin-binding proteins (ABPs) have been identified, yet their functions remain unclear. The present study reports the use of maize abp1, abp4, and abp1abp4 mutants to investigate role ABPs during growth development. Single double abp mutant plants grown in a greenhouse differ from wild type (WT) leaf declination blade growth. effect dark (D), blue light (BL), red (RL), exogenous auxin on development seedlings was also studied. Relative WT, etiolated were shorter...