A5059631258- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Plant Disease Resistance and Genetics
- Plant-Microbe Interactions and Immunity
- Light effects on plants
- Microbial Community Ecology and Physiology
- Microbial bioremediation and biosurfactants
- Plant Water Relations and Carbon Dynamics
- Plant Reproductive Biology
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Resistance
- Soil and Water Nutrient Dynamics
- Water Treatment and Disinfection
- Wastewater Treatment and Nitrogen Removal
- Plant Parasitism and Resistance
- Plant responses to elevated CO2
- Microbial Metabolites in Food Biotechnology
- Plant Stress Responses and Tolerance
- Groundwater and Isotope Geochemistry
- Plant Micronutrient Interactions and Effects
- Soil Carbon and Nitrogen Dynamics
- Remote Sensing in Agriculture
- Nitrogen and Sulfur Effects on Brassica
- Groundwater flow and contamination studies
University of Sheffield
2015-2025
University of Southampton
1998
University of California, Los Angeles
1990-1991
University of Wales
1989-1991
University of Cambridge
1987-1989
Abstract The rhizobiome is an important regulator of plant growth and health. Plants shape their communities through production release primary secondary root metabolites. Benzoxazinoids (BXs) are common tryptophan-derived metabolites in grasses that regulate belowground aboveground biotic interactions. In addition to biocidal activity, BXs can plant–biotic interactions as semiochemicals or within-plant defence signals. However, the full extent mechanisms by which root-associated microbiome...
Summary Rhizosphere chemistry is the sum of root exudation chemicals, their breakdown products and microbial soil‐derived chemicals. To date, most studies about are based on sterile cultivation systems, which limits discovery that act as semiochemicals shape rhizosphere communities. Here, we present a method for untargeted metabolic profiling non‐sterile soil. We have developed an experimental growth system enables collection analysis chemicals from different plant species. High‐throughput...
Abstract Albugo candida (Pers.) (O.) Kunze is a biotrophic pathogen which infects the crucifer Arabidopsis thaliana (L.) Heynh forming discrete areas of infection. Eight days after inoculation leaves, white blisters became visible on under surface leaf although no symptoms were apparent upper surface. By day 14, region invaded by fungal mycelium had become chlorotic. Recently it has been hypothesized that an accumulation soluble carbohydrates, following increase in invertase activity, may...
The protist Plasmodiophora brassicae is a soil-borne pathogen of cruciferous species and the causal agent clubroot disease Brassicas including agriculturally important crops such as canola/rapeseed (Brassica napus). P. has remained an enigmatic plant rare example obligate biotroph that resides entirely inside host cell. cause severe yield losses can render infested fields unsuitable for Brassica crop growth due to persistence resting spores in soil up 20 years. To provide insight into...
The formation of stomata and leaf mesophyll airspace must be coordinated to establish an efficient robust network that facilitates gas exchange for photosynthesis, however the mechanism by which this development occurs remains unclear. Here, we combine microCT analyses with measures stomatal size patterning in a range wild, domesticated transgenic lines wheat Arabidopsis show is linked function both monocots eudicots. Our results support hypothesis flux via pores influences degree spatial...
Chlorophyll fluorescence imaging is a non-invasive, non-destructive means with which to examine the impact of fungal pathogens on photosynthetic metabolism host plants. As such, it has great potential for screening purposes in high-throughput phenomics environments. However, there diversity responses plants different plant-fungal and choice suitable experimental conditions protocols interpretation results requires both preliminary laboratory experiments an understanding biology specific...
The formation of biofilms by diverse bacteria isolated from contaminated soil and groundwater on model substrata with different surface properties was assessed in a multifactorial screen. Diverse attachment phenotypes were observed as measured crystal violet dye retention confocal laser scanning microscopy (CLSM). Bulk measurements cell hydrophobicity had little predictive ability determining whether would develop hydrophobic or hydrophilic substrata. Therefore selected pairs the genera...
Plasmodiophora brassicae (clubroot) infection leads to reprogramming of host development resulting in the formation characteristic galls. In this work we explored cellular events that underly gall Arabidopsis thaliana with help molecular markers cell division (CYCB1:GUS) and meristematic activity (ANT:GUS). Our results show involved amplification existing activities within vascular cambium (VC) phloem parenchyma (PP) cells region hypocotyl. Additionally found increase VC prolonged...
Summary The pattern of cell division, growth and separation during leaf development determines the volume airspace in a leaf. resulting balance cellular material is expected to significantly influence primary function leaf, photosynthesis, yet manner degree which division patterns affect networks photosynthesis remains largely unexplored. In this paper we investigate relationship size patterning, by promoting repressing expression cycle genes mesophyll. Using micro CT imaging quantify...
Successful biotrophic plant pathogens can divert host nutrition toward infection sites. Here we describe how the protist Plasmodiophora brassicae establishes a long-term feeding relationship with its by stimulating phloem differentiation and phloem-specific expression of sugar transporters within developing galls. Development galls in infected Arabidopsis (Arabidopsis thaliana) plants is accompanied stimulation BREVIS RADIX, COTYLEDON VASCULAR PATTERN, OCTOPUS gene leading to an increase...
Clubroot (Plasmodiophora brassicae) is a pathogen of Brassicaceae that causes significant reductions in yield as consequence gall formation the root and hypocotyl infected plants. The hijacks host vascular cambium development, cytokinins are implicated this process. This paper uses transcriptomics metabolomics to investigate changes cytokinin metabolism during clubroot-infected Arabidopsis thaliana. RNASeq analysis tissue showed was strongly down-regulated both at onset late stages...
Plant roots are complex, three-dimensional structures that play a central role in anchorage, water and nutrient acquisition, storage interaction with rhizosphere microbes. Studying the development of plant root system architecture is inherently difficult as soil not transparent medium.This study uses electrical impedance tomography (EIT) to visualise oilseed rape horticultural compost. The healthy, control plants those infected gall-forming pathogen, Plasmodiophora brassicae-the causative...
The use of spectral imaging within the plant phenotyping and breeding community has been increasing due its utility as a non-invasive diagnostic tool. However, there is lack systems targeted specifically at science duties, resulting in low precision for canopy-scale measurements. This study trials prototype multispectral system designed studies looks an early detection visually asymptomatic disease phases, this case Pyrenopeziza brassicae Brassica napus. analysis takes advantage machine...
Plant based biochars are proposed as soil amendments to immobilize potentially toxic trace elements (PTEs), such Cd(II), Pb(II) and Zn(II) aid in restoration. However, the sorption capacity of biochar for these can vary widely depending on nature metal properties. Currently, there is no clear methodology pre-screen their suitability adsorbents elements. Therefore, facilitate selection application restoration, this study explored relationships between physico-chemical properties five...
Avoiding microbial contamination and biofilm formation on the surfaces of aircraft fuel tanks is a major challenge in aviation industry. The inevitable presence water systems nutrients provided by makes an ideal environment for bacteria, fungi, yeast to grow. Understanding how microbes grow different tank materials first step control systems. In this study, biofilms Pseudomonas putida, model Gram-negative bacterium previously found tanks, were characterized aluminum 7075-T6 surfaces, which...
Abstract In common with many other higher plant species, Arabidopsis undergoes photosynthetic acclimation, altering the composition of apparatus in response to fluctuations its growth environment. The changes function that result from acclimation can be detected a noninvasive manner by monitoring chlorophyll (Chl) fluorescence. This technique has been used develop screen enables rapid identification plants defective atACCLIMATION OF PHOTOSYNTHESIS TO THE ENVIRONMENT(APE) loci. application...