A5077951124- Extracellular vesicles in disease
- Advanced Proteomics Techniques and Applications
- Pregnancy and preeclampsia studies
- MicroRNA in disease regulation
- Mass Spectrometry Techniques and Applications
- Metabolomics and Mass Spectrometry Studies
- Bacteriophages and microbial interactions
- Plant Virus Research Studies
- Analytical Chemistry and Chromatography
- Genomics and Phylogenetic Studies
- Biomedical Text Mining and Ontologies
- Pharmacological Effects and Assays
- Analytical Methods in Pharmaceuticals
- Microbial Metabolic Engineering and Bioproduction
- Plant-Microbe Interactions and Immunity
- RNA and protein synthesis mechanisms
- Glycosylation and Glycoproteins Research
- Hemophilia Treatment and Research
- Machine Learning in Bioinformatics
- Cell death mechanisms and regulation
- Fungal and yeast genetics research
- Photosynthetic Processes and Mechanisms
- RNA regulation and disease
- Molecular Biology Techniques and Applications
- T-cell and B-cell Immunology
Centro Nacional de Biotecnología
2012-2022
Instituto de Salud Carlos III
2017-2021
Umeå University
2021
Universidad de Málaga
2018
Consejo Superior de Investigaciones Científicas
2009-2016
hCLE/C14orf166 is a nuclear and cytoplasmic protein that interacts with the RNAP II, modulates RNA metabolism present in granules involved localized translation. Here we have studied whether hCLE shares common interactors nucleus cytosol, which could shed light on its participation sequential phases of metabolism. Nuclear purified hCLE-associated factors were identified proteins mRNA metabolism, motor-related proteins, cytoskeletal translation-related found. Purified complexes also contain...
The high incidence of pre-eclampsia, which affects 2-7% all pregnancies, remains a major health concern. Detection pre-eclampsia before the appearance clinical symptoms is essential to allow early intervention, and would benefit from identification plasma/serum biomarkers help guide diagnosis treatment. Liquid biopsy has emerged as promising source protein that circumvents some inherent challenges proteome-wide analysis plasma/serum. In this respect, purified exosomes have added being...
Volatile compounds (VCs) emitted by phylogenetically diverse microorganisms (including plant pathogens and microbes that do not normally interact mutualistically with plants) promote photosynthesis, growth, the accumulation of high levels starch in leaves through cytokinin (CK)-regulated processes. In Arabidopsis (Arabidopsis thaliana) plants exposed to VCs, plastidic phosphoglucose isomerase (pPGI) acts as an important determinant photosynthesis likely a consequence its involvement...
Tandem mass spectrometry-based proteomics is currently in great demand of computational methods that facilitate the elimination likely false positives peptide and protein identification. In last few years, a number new identification programs have been described, but scores or other significance measures reported by these cannot always be directly translated into an easy to interpret error rate measurement such as discovery rate. this work we used generalized lambda distributions model...
Transition from symmetric to asymmetric cell division requires precise coordination of differential gene expression. We show that embryonic stem cells (ESCs) mainly express DIDO3 and their differentiation after leukemia inhibitory factor withdrawal DIDO1 C-terminal truncation (Dido3ΔCT) impedes ESC while retaining self-renewal; small hairpin RNA-Dido1 ESCs have the same phenotype. Dido3ΔCT is rescued by ectopic expression DIDO3, which binds Dido locus via H3K4me3 RNA POL II induces DIDO1,...
The development of the HUPO-PSI's (Proteomics Standards Initiative) standard data formats and MIAPE (Minimum Information About a Proteomics Experiment) guidelines should improve proteomics sharing within scientific community. journals have encouraged use these standards to quality experimental reporting ease evaluation publication manuscripts. However, there is an evident lack bioinformatics tools specifically designed create edit file reports, or embed them workflows. In this article, we...
Phosphorylation and O-GlcNAcylation are two widespread post-translational modifications (PTMs), often affecting the same eukaryotic target protein. Plum pox virus (PPV) is a member of genus Potyvirus which infects wide range plant species. capsid protein (CP) PPV has been studied extensively, some evidence CP phosphorylation also reported. Here, we use proteomics analyses to demonstrate that phosphorylated in vivo at N-terminus beginning core region. In contrast with 'yin-yang' mechanism...
Monocytes are bone marrow-derived leukocytes that part of the innate immune system. divided into three subsets: classical, intermediate and non-classical, which can be differentiated by their expression some surface antigens, mainly CD14 CD16. These cells key players in inflammation process underlying mechanism many diseases. Thus, molecular characterization these may provide very useful information for understanding biology health disease. We performed a multicentric proteomic study with...
Eukaryotic proteins are often targets of posttranslational modifications (PTMs). Capsid protein (CP) plum pox virus (PPV), a member genus Potyvirus, has been reported to be prone phosphorylation in four serines at the N-terminal region. CP proposed influence PPV infection by regulating accumulation coordination with second PTM, O-GlcNAcylation. In this study, further proteomic characterization revealed additional phospho-targets, thus evidencing even greater complexity network PTMs affecting...
Mass-spectrometry-based proteomics has evolved into a high-throughput technology in which numerous large-scale data sets are generated from diverse analytical platforms. Furthermore, several scientific journals and funding agencies have emphasized the storage of public repositories to facilitate its evaluation, inspection, reanalysis.(1) As consequence, growing rapidly. However, tools needed integrate multiple compare different experimental features or perform quality control analysis. Here,...
Phosphorylation and O-GlcNAcylation are widespread post-translational modifications (PTMs), often sharing protein targets. Numerous studies have reported the phosphorylation of plant viral proteins. In plants, research on lags behind that other eukaryotes, information about O-GlcNAcylated proteins is extremely scarce. The potyvirus Plum pox virus (PPV) causes sharka disease in Prunus trees also infects a wide range experimental hosts. Capsid (CP) from virions PPV-R isolate purified...
The RcsC, RcsD, and RcsB proteins compose a system used by enteric bacteria to sense envelope stress. Signal transmission occurs from the sensor RcsC transcriptional regulator RcsB. Accessory proteins, such as IgaA, are known adjust response level. In previous transcriptomic study, we uncovered 85 genes differentially expressed in Salmonella enterica serovar Typhimurium igaA mutants. Here, extended these observations proteomics performing differential isotope-coded protein labeling (ICPL)...
Global analysis of protein phosphorylation by mass spectrometry proteomic techniques has emerged in the last decades as a powerful tool biological and biomedical research. However, there are several factors that make global study phosphoproteome more challenging than measuring non-modified proteins. The low stoichiometry phosphorylated species need to retrieve residue specific information require particular attention on sample preparation, data acquisition processing ensure reproducibility,...