A5032052287- Receptor Mechanisms and Signaling
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Ion channel regulation and function
- Protein Hydrolysis and Bioactive Peptides
- Alzheimer's disease research and treatments
- Extracellular vesicles in disease
- Mass Spectrometry Techniques and Applications
- Parkinson's Disease Mechanisms and Treatments
- Redox biology and oxidative stress
- Advanced Proteomics Techniques and Applications
- Cell Adhesion Molecules Research
- Algal biology and biofuel production
- Neuroscience and Neuropharmacology Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Amyotrophic Lateral Sclerosis Research
- Aldose Reductase and Taurine
- Reproductive biology and impacts on aquatic species
- Monoclonal and Polyclonal Antibodies Research
- Nicotinic Acetylcholine Receptors Study
- Sulfur Compounds in Biology
- Selenium in Biological Systems
- Insect Resistance and Genetics
- Biomedical Research and Pathophysiology
- Supramolecular Self-Assembly in Materials
Universidad Autónoma de Madrid
1990-2025
Centro de Biología Molecular Severo Ochoa
1993-2025
Consejo Superior de Investigaciones Científicas
2006-2024
Centro Nacional de Biotecnología
2021
Instituto de Salud Carlos III
2021
Umeå University
2021
Universidade Federal de Santa Catarina
1993
Alzheimer's disease is a progressive neurodegenerative disorder that leads to dementia mainly among the elderly. This characterized by presence in brain of amyloid plaques and neurofibrillary tangles provoke neuronal cell deat
The impact of the world’s growing population on food systems and role dietary patterns in management non-communicable diseases underscore need to explore sustainable protein sources. Although microalgae have stood out as alternative sources proteins bioactive peptides, some species such Nannochloropsis gaditana remain unexplored. This study aimed characterize N. gaditana’s proteome evaluate its potential a source peptides by using an silico approach. A total 1955 were identified classified...
Among neurogenerative diseases, amyotrophic lateral sclerosis (ALS) is a fatal illness characterized by progressive motor neuron dysfunction in the cortex, brainstem and spinal cord.ALS most common form of disease; yet, to date, exact etiology ALS remains unknown.In present work, we have explored possibility fungal infection cerebrospinal fluid (CSF) brain tissue from patients.Fungal antigens, as well DNA several fungi, were detected CSF patients.Additionally, examination sections frontal...
Abstract Tetraspanins are often used as Extracellular Vesicle (EV) detection markers because of their abundance on these secreted vesicles. However, data function EV biogenesis controversial and compensatory mechanisms occur upon gene deletion. To overcome this handicap, we have compared the effects tetraspanin CD9 deletion with those elicited by cytopermeable peptides blocking properties against CD9. Both peptide or reduced number early endosomes. induced an increase in lysosome numbers,...
Sacha Inchi (
Downstream regulatory element antagonist modulator (DREAM)/potassium channel interacting protein (KChIP3) is a multifunctional of the neuronal calcium sensor subfamily Ca2+-binding proteins with specific roles in different cell compartments. In nucleus, DREAM acts as Ca2+-dependent transcriptional repressor, and outside nucleus interacts Kv4 potassium channels, regulating their trafficking to membrane gating properties. this study we characterized interaction GRK6 GRK2, members G...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTLocalization of the strychnine binding site on 48-kilodalton subunit glycine receptorAna Ruiz-Gomez, Esperanza Morato, Margarita Garcia-Calvo, Fernando Valdivieso, and Federico Mayor, Jr.Cite this: Biochemistry 1990, 29, 30, 7033–7040Publication Date (Print):July 31, 1990Publication History Published online1 May 2002Published inissue 31 July 1990https://pubs.acs.org/doi/10.1021/bi00482a012https://doi.org/10.1021/bi00482a012research-articleACS...
Abstract Ribbon worms are active predators that use an eversible proboscis to inject venom into their prey and defend themselves with toxic epidermal secretions. Previous work on nemertean has largely focused just a few species not investigated the different predatory defensive secretions in detail. Consequently, our understanding of composition evolution ribbon worm venoms is still very limited. Here, we present comparative study combining RNA-seq differential gene expression analyses...
The innate and adaptative immune systems are involved in the regulation of inflammatory oxidative processes mediators such as reactive oxygen species (ROS) nitric oxide (NO). exacerbated action these players results an stress status chronic inflammation, which is responsible for development non-communicable diseases (NCDs). By modulating mediators, bioactive compounds food can exert a key role prevention several NCDs. Among compounds, soybean proteins peptides lunasin have been considered to...
Abstract Background The lack of standardized protocols for isolating extracellular vesicles (EVs), especially from biobank-stored blood plasma, translates to limitations the study new biomarkers. This examines whether a combination current isolation methods could enhance specificity and purity isolated EVs diagnosis personalized medicine purposes. Results were healthy human plasma stored one year by ultracentrifugation (UC), size exclusion chromatography (SEC), or SEC UC combined (SEC + UC)....
All animals are capable of undergoing gametogenesis. The ability forming haploid cells from diploid through meiosis and recombination appeared early in eukaryotes, whereas further gamete differentiation is mostly a metazoan signature. Morphologically, the gametogenic process presents many similarities across animal taxa, but little known about its conservation at molecular level. Porifera earliest divergent therefore an ideal phylum to understand evolution toolkits. Although sponge...
Leishmania infantum causes visceral leishmaniasis (kala-azar), the most severe form of leishmaniasis, which is lethal if untreated. A few years ago, re-sequencing and de novo assembling L. (JPCM5 strain) genome was accomplished, now we aimed to describe characterize experimental proteome this species. In work, performed a proteomic analysis from axenic cultured promastigotes carried out detailed comparison with other proteomes published date. We identified 2352 proteins based on search mass...
E3 ubiquitin ligases mediate the last step of ubiquitination pathway in ubiquitin-proteasome system (UPS). By targeting transcriptional regulators for their turnover, E3s play a crucial role every aspect plant biology. In plants, SKP1/CULLIN1/F-BOX PROTEIN (SCF)-type are essential perception and signaling several key hormones including auxins jasmonates (JAs). F-box proteins, TRANSPORT INHIBITOR RESPONSE 1 (TIR1) CORONATINE INSENSITIVE (COI1), bind directly repressors AUXIN/INDOLE-3-ACETIC...
In this study, we have compared the cellular pathology associated with β‐amyloid (βA) deposits which characterize Alzheimer's disease (AD) in demented patients pathologically confirmed AD, that non‐demented aged individuals. Brain sections from two severely AD cases, six individuals βA deposits, and age‐matched controls devoid of were double‐immunostained antibodies against βA, antibody markers for neurofibrillary tangles (NFT), astrocytes microglial cells. We found displayed numerous...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTFunctional reconstitution of the glycine receptorMargarita Garcia-Calvo, Ana Ruiz-Gomez, Jesus Vazquez, Esperanza Morato, Fernando Valdivieso, and Federico Mayor, Jr.Cite this: Biochemistry 1989, 28, 15, 6405–6409Publication Date (Print):July 25, 1989Publication History Published online1 May 2002Published inissue 25 July 1989https://doi.org/10.1021/bi00441a037RIGHTS & PERMISSIONSArticle Views99Altmetric-Citations25LEARN ABOUT THESE METRICSArticle...
Nitric oxide (NO) is well established as a regulator of neurogenesis. NO increases the proliferation neural stem cells (NSC), and essential for hippocampal injury-induced neurogenesis following an excitotoxic lesion. One mechanisms underlying non-classical cell signaling protein S-nitrosylation. This post-translational modification consists in formation nitrosothiol group (R-SNO) cysteine residues, which can promote other oxidative modifications those residues. S-nitrosylation regulate many...
Leishmania infantum is causative of visceral leishmaniasis (kala-azar), the most severe form leishmaniasis, lethal if untreated. Few years ago, re-sequencing and de novo assembling L. (strain JPCM5) genome was accomplished, now we aimed to describe characterize experimental proteome this species. In work, have performed a proteomic analysis from axenic cultured promastigotes carried out detailed comparison with other proteomes published date. We identified 2,352 proteins based on search mass...
We have investigated the effect of chemical reagents that modify sulfhydryl groups on ligand binding properties glycine receptor (GlyR). The Hill coefficient (nH) for displacement [3H]strychnine by was increased from approximately 0.8 to values significantly above 1 (approximately 1.2-1.4) in membranes pretreated with disulfide-reducing agent dithiothreitol or glutathione. However, affinity strychnine GlyR not affected these treatments. This indicates several sites interact cooperatively...
Using ‘inside‐out’ membrane patches obtained from reconstituted giant liposomes containing purified glycine receptor rat spinal cord, we have detected chloride currents elicited in response to the presence of agonists or β‐alanine. Regardless agonist employed, two different patterns single channel could be detected, which differ their main conductance, complexity substates and opening frequency. In agreement with expectations heterogeneity suggested recently at mRNA cDNA level, our results...
The β‐amyloid peptide (Aß) is a 4 kDa proteolytic fragment derived from the precursor protein (βAPP) which deposited as amyloid fibrils in brains of patients with Alzheimer's disease. βAPP processing was investigated C6 glioma cells using several affinity‐purified anti‐peptide antibodies raised against different domains protein. Both direct immunoblot analysis conditioned medium and metabolic labeling followed by immunoprecipitation extracellular specific revealed that these glial normally...
The high-throughput proteomics data generated by increasingly more sensible mass spectrometers greatly contribute to our better understanding of molecular and cellular mechanisms operating in live beings. Nevertheless, analyses are based on accurate genomic protein annotations, some information may be lost if these resources incomplete. Here, we show that most recovered interconnecting genomics approaches (i.e., following a proteogenomic strategy), resulting, turn, an improvement...