A5040668425- Salmonella and Campylobacter epidemiology
- Vibrio bacteria research studies
- Bacterial Genetics and Biotechnology
- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Biochemical and Structural Characterization
- Microbial Metabolic Engineering and Bioproduction
- Microbial Metabolism and Applications
- Listeria monocytogenes in Food Safety
- Microbial Inactivation Methods
- Enzyme Structure and Function
- Viral gastroenteritis research and epidemiology
- RNA and protein synthesis mechanisms
- Infective Endocarditis Diagnosis and Management
- Escherichia coli research studies
- Botanical Research and Chemistry
- Advanced Proteomics Techniques and Applications
- Essential Oils and Antimicrobial Activity
- Botanical Research and Applications
- Bacterial Identification and Susceptibility Testing
- Enzyme Production and Characterization
- Aquaculture disease management and microbiota
- Amino Acid Enzymes and Metabolism
- Mass Spectrometry Techniques and Applications
- Cancer Research and Treatments
Instituto de Biología Molecular y Celular de Rosario
2015-2024
National University of Rosario
2015-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2015
Centro Nacional de Biotecnología
2007-2014
Consejo Superior de Investigaciones Científicas
2008-2014
Universidad Autónoma de Madrid
2009
National University of Río Cuarto
2002
We describe a transcriptomic study of the effect hha and ydgT mutations in Salmonella enterica serovar Typhimurium. A large number genes showing altered expression are located AT-rich horizontally acquired DNA sequences. Many these have also been reported to be targets for H-NS. As Hha YdgT interact with H-NS, our findings strongly suggest that and/or must form complexes H-NS when they silence regions.
ABSTRACT Intracellular growth attenuator A (IgaA) was identified as a Salmonella enterica regulator limiting bacterial inside fibroblasts. Genetic evidence further linked IgaA to repression of the RcsCDB regulatory system, which responds envelope stress. How attenuates this system is unknown. Here, we present genome expression profiling data S. serovar Typhimurium igaA mutants grown at high osmolarity and displaying exacerbated Rcs responses. Transcriptome revealed that gene changes...
Unsaturated fatty acids promote Salmonella infection by directly repressing the transmembrane sensor PhoQ.
ABSTRACT PrtA is the major secreted metalloprotease of Serratia marcescens . Previous reports implicate in pathogenic capacity this bacterium. also clinically used as a potent analgesic and anti-inflammatory drug, its catalytic properties attract industrial interest. Comparatively, there scarce knowledge about mechanisms that physiologically govern expression In work, we demonstrate production derepressed when bacterial growth temperature decreases from 37°C to 30°C. We show thermoregulation...
Sortases are bacterial enzymes that anchor surface proteins covalently to the peptidoglycan upon cleavage of a motif located at their C-terminal end. Motifs recognized by sortases class-B (SrtB) defined consensus sequence NP(Q/K)(T/S)(N/G/S)(D/A). Evidence supporting this is limited IsdC Staphylococcus aureus and Bacillus anthracis, cleaved motifs NPQTN NPKTG, respectively. In Listeria monocytogenes, StrB has two substrates, Lmo2185 Lmo2186, containing NAKTN NKVTN (or overlapping NPKSS) as...
IgaA is a Salmonella enterica membrane protein that attenuates the response of RcsCDB signalling system to envelope stress. This essential unless inactivated, suggesting may constantly adjust magnitude response. Such functional link also supported by concurrence igaA and rcsD-rcsB-rcsC loci in genomes enteric bacteria selection spontaneous mutations following deprivation. However, exact nature rendering dispensable remains undefined. In this work, we examined how transduction an null allele...
Bacteria of the genus Listeria contain largest family LPXTG surface proteins covalently anchored to peptidoglycan. The extent which these may function or be regulated cooperatively is at present unknown. Because their unique cellular location, we reasoned that distinct could act as elements contributing cell wall homeostasis influencing stability other bound To test this hypothesis, used proteomics analyse mutants intracellular pathogen monocytogenes lacking implicated in pathogen-host...
ABSTRACT Serratia marcescens strains are ubiquitous bacteria isolated from environmental niches and also constitute emergent nosocomial opportunistic pathogens. Here, we report on the draft genome sequence of S. strain RM66262, which was a patient with urinary tract infection in Bacteriology Service Rosario National University, Rosario, Argentina.
ABSTRACT The gene for glycine betaine transmethylase ( gbt ) was identified in Pseudomonas aeruginosa strain Fildes III by biochemical, physiological, and molecular approaches. Based on sequence analysis, the knockout corresponded to an open reading frame (ORF) named PA3082 genome of P. PAO1. translated product this ORF displayed similarity transferases different microorganisms. Mutation blocked utilization choline as carbon nitrogen sources.
The RcsC, RcsD, and RcsB proteins compose a system used by enteric bacteria to sense envelope stress. Signal transmission occurs from the sensor RcsC transcriptional regulator RcsB. Accessory proteins, such as IgaA, are known adjust response level. In previous transcriptomic study, we uncovered 85 genes differentially expressed in Salmonella enterica serovar Typhimurium igaA mutants. Here, extended these observations proteomics performing differential isotope-coded protein labeling (ICPL)...
Serratia marcescens is an enteric bacterium that can function as opportunistic pathogen with increasing incidence in clinical settings. This mainly due to the ability express a wide range of virulence factors and acquisition antibiotic resistance mechanisms. For these reasons, S. has been declared by World Health Organization (WHO) research priority develop alternative antimicrobial strategies. In this study, we found PhoP-binding motif promoter region transcriptional regulator RamA RM66262....
ABSTRACT Serratia marcescens , a member of the Enterobacteriaceae family, is an opportunistic human pathogen and frequent cause urinary tract infections. Clinical isolates often exhibit resistance to multiple antibiotics, posing challenges for successful treatment. Understanding its pathogenic mechanisms crucial elucidating new potential targets develop effective therapeutic interventions manage S. This work identifies u rea- i nduced l actonase S erratia (UilS), lactonase encoded in RM66262...