A5088467274- Lung Cancer Research Studies
- RNA modifications and cancer
- Lung Cancer Treatments and Mutations
- Peptidase Inhibition and Analysis
- Adipose Tissue and Metabolism
- Advanced Proteomics Techniques and Applications
- Adipokines, Inflammation, and Metabolic Diseases
- Pancreatic function and diabetes
- Antioxidant Activity and Oxidative Stress
- Vitamin D Research Studies
- Histone Deacetylase Inhibitors Research
- Cholesterol and Lipid Metabolism
- Metabolomics and Mass Spectrometry Studies
- Lipid metabolism and biosynthesis
- Epigenetics and DNA Methylation
- Regulation of Appetite and Obesity
- Diabetes and associated disorders
- Cancer therapeutics and mechanisms
- Glycosylation and Glycoproteins Research
- Body Contouring and Surgery
- Vitamin C and Antioxidants Research
- Protein Degradation and Inhibitors
- Chromatin Remodeling and Cancer
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Molecular Biology Techniques and Applications
Institut d'Investigació Biomédica de Bellvitge
2013-2021
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2004-2013
Instituto de Investigación Sanitaria Aragón
2009-2013
Instituto Aragonés de Ciencias de la Salud
2013
Instituto de Salud Carlos III
2013
Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas
2010-2012
Universitat de Barcelona
2004-2009
Hospital Clínic de Barcelona
2007
Zero to Three
2005
Recent efforts to sequence human cancer genomes have highlighted that point mutations in genes involved the epigenetic setting occur tumor cells. Small cell lung (SCLC) is an aggressive with poor prognosis, where little known about genetic events related its development. Herein, we identified presence of homozygous deletions candidate histone acetyltransferase KAT6B, and loss corresponding transcript, SCLC lines primary tumors. Furthermore, show, vitro vivo, depletion KAT6B expression...
A role for cytokine regulated proteins in epithelial cells has been suggested the pathogenesis of inflammatory bowel diseases (IBD). The aim this study was to identify such targets using a proteomic functional approach. Protein patterns from (35)S-radiolabeled homogenates cultured colon were compared before and after exposure interferon-gamma, interleukin-1beta interleukin-6. Proteins separated by two-dimensional polyacrylamide gel electrophoresis. Both autoradiographies silver stained gels...
Sirtuins are NAD+-dependent deacetylases that facilitate cellular stress response. They include SirT6, which protects genome stability and regulates metabolic homeostasis through gene silencing, whose loss induces an accelerated aging phenotype directly linked to hyperactivation of the NF-κB pathway. Here we show SirT6 binds H3K9me3-specific histone methyltransferase Suv39h1 monoubiquitination conserved cysteines in PRE-SET domain Suv39h1. Following activation signaling is released from IκBα...
A fast and sensitive high performance liquid chromatography with atmospheric pressure chemical ionization tandem mass spectrometry (HPLC-APCI-MS/MS) method to identify quantify oxysterols, phytosterols non-cholesterol sterols at the pico-molar concentration level in human serum only one run was developed this study. This allows simultaneous separation quantitation of individual phytosterols, cholesterol precursors oxidized derivatives without a derivatization step single run, thus providing...
The increasing worldwide incidence of obesity and the limitations current treatments raise need for finding novel therapeutic approaches to treat this disease. purpose study was first investigate effects tungstate on body weight insulin sensitivity in a rat model diet-induced obesity. Second, we aimed gain insight into molecular mechanisms underlying its action. Oral administration significantly decreased adiposity without modifying caloric intake, intestinal fat absorption, or growth rate...
Several studies have demonstrated the benefits of massive weight reduction following Roux-en-Y gastric bypass (RYGBP) on metabolic syndrome (1–3). However, time course effects RYGBP components and its potential underlying mechanisms has not been reported. In recent years, it proposed that low-grade chronic inflammation is a critical factor (4–6). Alternatively, would be better explained by combination factors including but limited to systemic (7). The aim our study was evaluate components,...
Abstract Background Protein phosphatase 2A (PP2A) is a family of conserved serine/threonine phosphatases involved in several essential aspects cell growth and proliferation. PP2ACdc55 has been extensively related to cycle events budding yeast; however, few substrates have identified. Here, we performed quantitative mass spectrometry approach reveal new PP2A-related processes mitotic arrested cells. Results We identified 62 statistically significant mainly actin-cytoskeleton organization. In...
Our previous results demonstrated that tungstate decreased weight gain and adiposity in obese rats through increased thermogenesis lipid oxidation, suggesting brown adipose tissue was one of the targets its antiobesity effect. To identify potential tungstate, we used DIGE to compare protein extracts from following experimental groups: untreated lean, tungstate-treated obese, rats. distinguish direct action those are secondary body loss, also included analysis an additional group consisting...
The Spanish team of the Human Proteome Project (SpHPP) marked annotation Chr16 and data analysis as one its priorities. Precise Chromosome 16 proteins according to C-HPP criteria is presented. Moreover, Body Map 2.0 RNA-Seq Encyclopedia DNA Elements (ENCODE) sets were used obtain further information relative cell/tissue specific chromosome coding gene expression patterns infer presence missing proteins. Twenty-four shotgun 2D-LC-MS/MS gel/LC-MS/MS MIAPE compliant experiments, representing...
Adipose tissue plays an active role in the development of obesity, and thus characterization molecular changes related to obesity this is a priority. Recently, we identified tungstate as potent body weight reducing agent obese animals, adipose being one targets its action. In study proteomics approach combining 2-DE MS was used identify proteins associated with white tissue. Twenty-nine were found differentially expressed between lean diet-induced rats. Expression transferrin, vimentin,...
Obesity has emerged as one of the major global epidemics 21st century and is now reaching alarming proportions. Obese subjects have an increased morbidity mortality, decreased quality life a risk developing pathologies such diabetes mellitus, insulin resistance cardiovascular disease. complex disease characterised by increase in body fat mass resulting from imbalance between energy intake expenditure. Signal integration adipose tissue, other peripheral organs CNS seems to regulate...
Sodium tungstate is an anti-obesity drug targeting peripheral tissues. In vivo, sodium reduces body weight gain and food intake through increasing energy expenditure lipid oxidation, but it also modulates hypothalamic gene expression when orally administered, raising the possibility of a direct effect on central nervous system.Sodium was administered intraperitoneally (ip) to Wistar rats, its levels were measured in cerebrospinal fluid mass spectrometry. Body monitored for 24 h after...
This study aims at exploring the effects of sodium tungstate treatment on hypothalamic plasticity, which is known to have an important role in control energy metabolism.Adult lean and high-fat diet-induced obese mice were orally treated with tungstate. Arcuate paraventricular nuclei lateral hypothalamus separated subjected proteomic analysis by DIGE mass spectrometry. Immunohistochemistry vivo magnetic resonance imaging also performed.Sodium reduced body weight gain, food intake, blood...
Many studies have shown both the CD28—D80/86 costimulatory pathway and PD-1—PD-L1/L2 coinhibitory to be important signals in modulating or decreasing inflammatory profile ischemia-reperfusion injury (IRI) a solid organ transplant setting. The importance of these two opposing pathways their potential synergistic effect led our group design human fusion recombinant protein with CTLA4 PD-L2 domains named HYBRI. objective study was determine HYBRI binding postulated ligands (CD80) (PD-1) using...
Global analysis of protein phosphorylation by mass spectrometry proteomic techniques has emerged in the last decades as a powerful tool biological and biomedical research. However, there are several factors that make global study phosphoproteome more challenging than measuring non-modified proteins. The low stoichiometry phosphorylated species need to retrieve residue specific information require particular attention on sample preparation, data acquisition processing ensure reproducibility,...
We have previously reported the identification of a pancreata mitoxantrone-resistant cell population which expressed ABCG2 transporter with pancreatic stellate cells phenotype (PaSC) and ability secreting insulin after inducing their differentiation. The characterization secretome this by two-dimensional electrophoresis (2D) coupled mass spectrometry MALDI-TOF was able to identify seventy-six protein spots involved in different cellular processes: development/differentiation, proteases,...