A5062278316- Renal Transplantation Outcomes and Treatments
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Pharmacological Effects and Toxicity Studies
- Cancer, Hypoxia, and Metabolism
- HIV/AIDS drug development and treatment
- Immune Cell Function and Interaction
- Autophagy in Disease and Therapy
- T-cell and B-cell Immunology
- Transplantation: Methods and Outcomes
- Mitochondrial Function and Pathology
- Cytomegalovirus and herpesvirus research
- Drug Transport and Resistance Mechanisms
- ATP Synthase and ATPases Research
- Metabolism, Diabetes, and Cancer
- Organ Transplantation Techniques and Outcomes
- Metal complexes synthesis and properties
- Systemic Lupus Erythematosus Research
- Cancer Immunotherapy and Biomarkers
- Ferrocene Chemistry and Applications
- Monoclonal and Polyclonal Antibodies Research
- Clinical Nutrition and Gastroenterology
- Signaling Pathways in Disease
- MicroRNA in disease regulation
- Neuroinflammation and Neurodegeneration Mechanisms
Universitat de Barcelona
2017-2024
Bellvitge University Hospital
2016-2024
Institut d'Investigació Biomédica de Bellvitge
2016-2024
Universidad de Burgos
2022-2024
In human cancers, transforming growth factor-β1 (TGF-β1) plays a dual role by acting as both tumor suppressor and promoter of metastasis. Although TGF-β1 contributes to the metabolic reprogramming cancer cells tumor-associated stromal cells, little is known molecular mechanisms connecting this cytokine with enhanced glycolysis. PFKFB3 homodymeric bifunctional enzyme, belonging family 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatases, that controls conversion fructose-6-phosphate...
Donor/recipient molecular human leukocyte antigen (HLA) mismatch predicts primary B-cell alloimmune activation, yet the impact on de novo donor-specific T-cell alloimmunity (dnDST) remains undetermined. The hypothesis of our study is that donor/recipient HLA mismatches assessed at level may also influence a higher susceptibility to development posttransplant alloimmunity. In this prospective observational study, 169 consecutive kidney transplant recipients without preformed antibodies (DSA)...
Introduction: Tacrolimus is the backbone immunosuppressant after solid organ transplantation. has a narrow therapeutic window with large intra- and inter-patient pharmacokinetic variability leading to frequent over- under-immunosuppression. While routine drug monitoring (TDM) remains standard of care, tacrolimus may be influenced by circadian rhythms. Our aim was analyze pharmacokinetic/pharmacodynamic profiles on rhythms comparing morning night doses twice-daily formulation. Methods: This...
The strategy of the concentration-dose (C/D) approach and different profiles tacrolimus (Tac) according to cytochrome P450 polymorphisms (CYPs) focus on metabolism Tac are proposed as tools for follow-up transplant patients. objective this study is analyse both strategies confirm whether stratification patients pharmacokinetic behaviour C/D corresponds classification their CYP3A4/5 cluster metabolizer profile. 425 kidney who received immunosuppressive treatment have been included....
Due to the relevance of lactic acidosis in cancer, several therapeutic strategies have been developed targeting its production and/or regulation. In this matter, inhibition approaches key proteins such as lactate dehydrogenase or monocarboxylate transporters showed promising results, however, metabolic plasticity and tumor heterogeneity limits their efficacy. study, we explored anticancer potential a new strategy based on disturbing permeability independently activity using small molecule...
An excessive production of lactate by cancer cells fosters tumor growth and metastasis. Therefore, targeting metabolism transport offers a new therapeutic strategy against cancer, based on dependency some for as energy fuel or oncogenic signal. Herein we present family anionophores the structure click-tambjamines that have proved to be extremely active carriers across phospholipid membranes. Compound 1, most potent transmembrane carrier, was studied in HeLa cells. The use monocarboxylate...
Patients undergoing conventional hemodialysis (C-HD) present a greater immuno-inflammatory state probably related to uremia, sympathetic nervous system (SNS) activation and /or membrane bioincompatibility, which could improve with technique-switching online hemodiafiltration (OL-HD). The antigen-independent pathway of this modified immunologic turns dendritic cells (DC) into an accurate cell model study these patients. aim is further evaluate the immune-inflammatory patients in C-HD assessed...
Systemic lupus erythematosus is a highly complex and heterogeneous autoimmune disease mostly mediated by B cells. It characterized circulating self-reactive antibodies that deposit form immune complexes in kidney, leading to irreparable tissue damage resulting nephritis. In New Zealand Black X White F1 mouse model, we tested two different small interfering RNA (siRNA) silencing treatments against interferon regulatory factor 5 (IRF5) cell-activating (BLYSS) expression their combination...
Overcoming resistance is one of the most challenging features in current anticancer therapy. Autophagy a cellular process that confers some advanced tumors, since it enables cancer cells to adapt stressful situations, such as treatments. Hence, inhibition this cytoprotective autophagy leads tumor sensitization and death. In regard, we designed novel potent anionophore compound specifically targets lysosomes, called LAI-1 (late-stage inhibitor-1), evaluated its role blocking potential effects...
Despite intensive monitoring of whole blood tacrolimus concentrations, acute rejection after kidney transplantation occurs during therapy. Intracellular concentrations could better reflect exposure at the site action and its pharmacodynamics (PD). pharmacokinetic (PK) profile following different formulations (immediate-release (TAC-IR) extended-release (TAC-LCP)) remains unclear. Therefore, aim was to study intracellular PK TAC-IR TAC-LCP correlation with (WhB) PD. A post-hoc analysis a...
A series of fluorescent coumarin-based bis-ureas have been synthesised. These compounds show strong HCl cotransport properties in model vesicle systems. Cellular localisation studies that cytotoxic compound 4 localises lysosomes and perturbs cellular pH.
Two ruthenium(II) polypyridyl complexes were prepared with the {Ru(phen)
The glycolytic modulator TP53-Inducible Glycolysis and Apoptosis Regulator (TIGAR) is overexpressed in several types of cancer has a role metabolic rewiring during tumor development. However, little known about the this enzyme proliferative tissues under physiological conditions. In current work, we analysed TIGAR primary human lymphocytes stimulated with mitotic agent Concanavalin A (ConA). We found that expression was induced through PI3K/AKT pathway, since Akti-1/2 LY294002 inhibitors...
Metastasis is the primary cause of death in cancer patients. Many current chemotherapeutic agents only show cytotoxic, but not antimetastatic properties. This leads to a reduction tumor size, allows cells disseminate, which ultimately causes patient death. Therefore, novel anticancer compounds with both effects need be developed. In this work, we analyze properties prodigiosin and obatoclax (GX15-070), drugs Prodiginines (PGs) family. We studied PGs’ on cellular adhesion morphology human...
The aim of the study is to develop a population pharmacokinetic (PopPK) model and investigate influence CYP3A5/CYP3A4 ABCB1 single nucleotide polymorphisms (SNPs) on Tacrolimus PK parameters after LCP-Tac formulation in stable adult renal transplant patients. was developed, using NONMEM v7.5, from full profiles clinical (n = 30) trough concentrations (C0) patient follow-up 68). profile best described by two-compartment with linear elimination, parameterized elimination (CL/F) distributional...
Many studies have shown both the CD28—D80/86 costimulatory pathway and PD-1—PD-L1/L2 coinhibitory to be important signals in modulating or decreasing inflammatory profile ischemia-reperfusion injury (IRI) a solid organ transplant setting. The importance of these two opposing pathways their potential synergistic effect led our group design human fusion recombinant protein with CTLA4 PD-L2 domains named HYBRI. objective study was determine HYBRI binding postulated ligands (CD80) (PD-1) using...
Tacrolimus (Tac) is the cornerstone calcineurin inhibitor in transplantation. Extended-release Meltdose formulation (Tac-LCP) offers better bioavailability compared with immediate-release (Tac-IR). We postulated that less fluctuating pharmacokinetic (PK) profile of Tac-LCP might maintain a sustained inhibition activity (CNA) between dose intervals. Higher concentrations (peak plasma concentration (Cmax )) after Tac-IR may not result more potent CNA due to capacity-limited effect. This study...
Five metal-arene complexes of formula [MX2(η6-p-cymene)(diR(1-pyrenyl)phosphane)] (M = Os or Ru, X Cl I, R isopropyl phenyl) and symbolized as MRX2 were synthesized fully characterized, namely OsiPrCl2, OsiPrI2, OsPhCl2, OsPhI2 RuPhI2. Furthermore, nine cyclometalated half-sandwich [MX-(η6-p-cymene)(k2C-diR(1-pyrenyl)phosphane)] [M(η6-p-cymene)(kS-dmso)(k2C-diR(1-pyrenyl)phosphane)]PF6 c-MRX prepared; hence, c-OsiPrCl, c-OsiPrI, c-OsiPrdmso, c-OsPhCl, c-OsPhI, c-OsPhdmso, c-RuPhCl, c-RuPhI...