A5074532581- Birth, Development, and Health
- Pluripotent Stem Cells Research
- Tissue Engineering and Regenerative Medicine
- Adipose Tissue and Metabolism
- Ovarian function and disorders
- Fatty Acid Research and Health
- 3D Printing in Biomedical Research
- Reproductive Biology and Fertility
- Gestational Diabetes Research and Management
- Pregnancy and preeclampsia studies
- Pancreatic function and diabetes
- Neurogenesis and neuroplasticity mechanisms
- Growth Hormone and Insulin-like Growth Factors
- Adipokines, Inflammation, and Metabolic Diseases
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Hormonal Regulation and Hypertension
- Cardiovascular Disease and Adiposity
- Eating Disorders and Behaviors
- Gut microbiota and health
- Congenital heart defects research
- Body Composition Measurement Techniques
- Vitamin D Research Studies
- Pregnancy-related medical research
- MicroRNA in disease regulation
Universitat de Barcelona
2017-2023
Centro de Investigación Biomédica en Red Diabetes y Enfermedades Metabólicas Asociadas
2018-2023
Instituto de Salud Carlos III
2018-2023
Hospital Sant Joan de Déu Barcelona
2017-2021
Sant Joan de Déu Research Foundation
2021
Consorci Institut D'Investigacions Biomediques August Pi I Sunyer
2014-2019
Instituto de Biologia Experimental e Tecnológica
2006-2012
Universidade Nova de Lisboa
2009-2012
Instituto Português Da Qualidade
2007-2010
Delft University of Technology
2004
The successful implementation of human embryonic stem cells (hESCs)-based technologies requires the production relevant numbers well-characterized and their efficient long-term storage. In this study, were microencapsulated in alginate to develop an integrated bioprocess for expansion cryopreservation pluripotent hESCs. Different three-dimensional (3D) culture strategies evaluated compared, specifically, microencapsulation hESCs as: i) single cells, ii) aggregates iii) immobilized on...
Objective CXCL14 (C-X-C motif chemokine ligand-14) is a released by active brown fat, showing protective effects against insulin resistance in experimental models. Polycystic ovary syndrome (PCOS) adolescent girls usually related to hepato-visceral fat excess and resistance, associates with comorbidities such as type 2 diabetes. Treatment low-dose combination of one antiandrogen antimineralocorticoid drug (spironolactone) two sensitizers (pioglitazone/metformin) (SPIOMET) particularly...
Primary cultures of brain cell neurospheres are valuable in vitro models for neurotoxicology and research. Such applications would greatly benefit from the development efficient cryopreservation protocols that assure availability viable genetically stable stocks functional neurospheres. In this work we aimed at developing an integrated strategy allowing long-term culture with high viability reduced recovery time postthawing. Microencapsulation clinical-grade, ultrahigh viscous, highly...
Hepato-visceral fat excess is a feature of polycystic ovary syndrome (PCOS). Risk factors for such include low prenatal weight gain and high postnatal gain. This study examined whether adolescent PCOS was preceded by relatively birth and/or BMI at diagnosis.Study participants included 467 girls with (298 without obesity 169 obesity), diagnosed, respectively, in Spain Germany; 87 healthy were controls. Z scores diagnosis derived, their differences calculated.Spanish German had mean z -0.7...
Abstract Adolescent polycystic ovary syndrome (PCOS) is characterized by androgen excess and oligomenorrhea, commonly driven hepato-visceral fat (“central obesity”) ensuing from a mismatch between prenatal postnatal nutrition, on background of genetic susceptibility. There no approved treatment for adolescent PCOS. We report the pooled results 2 pilot studies in nonobese girls with PCOS (N = 62, age 15.8 years) that compared effects randomized 1 year, either an oral estro-progestogen...
The commonly applied cryopreservation protocols routinely used in laboratories worldwide were developed for simple cell suspensions, and their application to complex systems, such as monolayers, tissues, or biosynthetic constructs, is not straightforward. In particular monolayer cultures, detachment membrane damage are often observed after cryopreservation. this work, combined strategies the of cells attached Matrigel-coated well plate's surfaces investigated based on entrapment...
Summary Background Girls with obesity and polycystic ovary syndrome (PCOS) women PCOS have altered gut microbiota. Objective To study the microbiota composition of girls without (age, 15.8 years; body mass index [BMI] 25 kg/m 2 ) effects randomized treatments an oral contraceptive (OC, N = 15) or spironolactone‐pioglitazone‐metformin (SPIOMET, for 1 year. Thirty‐one age‐matched served as controls. Methods 16S ribosomal subunit gene amplicon sequencing was performed in stool samples from all...
Inhibition of tyrosine phosphatase PTP1B in mouse and human islets promotes islet revascularization improves graft survival function.
Human embryonic stem cells (hESC) hold tremendous potential in the emerging fields of gene and cell therapy as well basic scientific research. One major challenges regarding their application is development efficient cryopreservation protocols for hESC since current methods present poor recovery rates and/or technical difficulties which impair effective processes that can handle bulk quantities pluripotent cells. The main focus this work was to compare different strategies adherent colonies....
Summary Background Insulin resistance and hepato‐visceral (central) fat excess are thought to contribute an earlier timing of adrenarche/pubarche puberty/menarche; this in turn relates often a mismatch between prenatal postnatal weight gain, which can be estimated by calculating the Z‐score change from birth (BW) body mass index (BMI) childhood. Methods We tested hypothesis that calculation may serve as proxy insulin adiposity prepuberty reappraising cohort children (mean age, 8.5 years),...
Summary S100A4 is a marker of subcutaneous adipose tissue dysfunction. Polycystic ovary syndrome (PCOS) often driven by hepato‐visceral adiposity. PCOS phenotypes are normalized more reduction central fat with spironolactone/pioglitazone/metformin (SPIOMET) than oral contraceptive (OC) treatment. We studied whether circulating concentrations high in adolescents and, if so, they normalize OC or SPIOMET. Assessments included S100A4, endocrine markers, body composition, abdominal partitioning...
Abstract Background A “mismatch” sequence of less prenatal weight gain and more postnatal may lead to ectopic lipid accumulation, trigger the development early adrenarche/pubarche activation gonadotropic axis resulting in puberty ending up full-blown adolescent polycystic ovary syndrome (PCOS). In present study, we assess whether a low-dose combination generics that collectively reduce fat through different pathways can slow down accelerated maturation girls with puberty. Methods Randomized,...
Abstract Background Polycystic ovary syndrome (PCOS) is the most prevalent, chronic endocrine-metabolic disorder of adolescents and young women (AYAs), affecting 5–10% AYAs worldwide. There no approved pharmacological therapy for PCOS. Standard off-label treatment with oral contraceptives (OCs) reverts neither underlying pathophysiology nor associated co-morbidities. Pilot studies have generated new insights into pathogenesis PCOS, leading to development a consisting fixed, low-dose...
Brown adipose tissue (BAT) is particularly abundant in neonates, but its association with measures of adiposity and metabolic health early infancy poorly delineated. Besides sustaining nonshivering thermogenesis, BAT secretes brown adipokines that act on systemic metabolism. The chemokine CXCL14 has been identified as a adipokine experimental studies.
Abstract Recent advances in cell‐based therapies require new approaches for cell cryopreservation, capable of dealing with large number samples and providing specific conditions each type. Reduction sample volume from the commonly used 1 mL to 25 µL 30‐well micro‐cryosubstrates improves cryopreservation by allowing automation, data handling access individual wells without thawing whole cryosubstrate. This system was evaluated storage Caco‐2 colon adenocarcinoma cells, which differentiate...
Studies regarding brown adipose tissue (BAT) activity in children are scarce due to the difficulties detecting BAT by imaging and need for elaborate techniques measure activity. Here, prepubertal born small gestational age (SGA, n=41) appropriate (AGA, n=45) were examined order investigate whether prematurity has an impact on This is of special interest since it been shown that important insulin sensitivity a healthy lipid profile, with SGA have corresponding metabolic impairments.