A5009251623- Cholesterol and Lipid Metabolism
- Fatty Acid Research and Health
- Lipid metabolism and biosynthesis
- Antioxidant Activity and Oxidative Stress
- Paraoxonase enzyme and polymorphisms
- Edible Oils Quality and Analysis
- Peroxisome Proliferator-Activated Receptors
- Liver Disease Diagnosis and Treatment
- Endoplasmic Reticulum Stress and Disease
- Folate and B Vitamins Research
- Cancer, Lipids, and Metabolism
- Diet, Metabolism, and Disease
- Adipose Tissue and Metabolism
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Nutritional Studies and Diet
- Iron Metabolism and Disorders
- Hibiscus Plant Research Studies
- RNA Research and Splicing
- Diet and metabolism studies
- Birth, Development, and Health
- Metabolomics and Mass Spectrometry Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Genomics, phytochemicals, and oxidative stress
- Trace Elements in Health
- Lipoproteins and Cardiovascular Health
Instituto de Salud Carlos III
2013-2024
Centro de Investigación y Tecnología Agroalimentaria de Aragón
2016-2024
Universidad de Zaragoza
2015-2024
Instituto de Investigación Sanitaria Aragón
2014-2024
Spanish Biomedical Research Centre in Physiopathology of Obesity and Nutrition
2016-2024
Centro de Investigación Biomédica en Red
2017-2020
Hospital Universitario Miguel Servet
2012-2017
University of Edinburgh
2015
Universidad de Oviedo
2012
Gobierno de Aragón
2008-2012
Trans-10, cis-12-conjugated linoleic acid (CLA)-enriched diets promote fatty liver in mice, while cis-9, trans-11-CLA ameliorates this effect, suggesting regulation of multiple genes. To test hypothesis, apoE-deficient mice were fed a Western-type diet enriched with isomers, and their hepatic gene expression was analyzed DNA microarrays. provide an initial screening candidate genes, only 12 remarkably modified between both CLA isomers considered confirmed by quantitative RT-PCR. Additionally...
Melatonin has antiproliferative properties in prostate cancer cells. reduces proliferation without increasing apoptosis, and it promotes cell differentiation into a neuroendocrine phenotype. Because cells displayed an androgen-independent growth high resistance to radiotherapy chemotherapy, the role of molecules that induce was questioned terms their usefulness as oncostatic agents. By using human epithelial androgen-dependent cells, melatonin drug-induced apoptosis studied after acute...
Background and Purpose Squalene, the main hydrocarbon in unsaponifiable fraction of virgin olive oil, is involved cholesterol synthesis it has been reported to own antiatherosclerotic antiesteatosic effects. However, squalene's role on lipid plasma parameters influence genotype this effect need be addressed. Experimental Approaches Three male mouse models (wild-type, Apoa1- Apoe- deficient) were fed chow semisynthetic diets enriched squalene provide a dose 1 g/kg during 11 weeks. After...
Hydroxytyrosol is a phenol found in olive oil. To verify the effect of hydroxytyrosol on development atherosclerosis, two groups apo E deficient male mice standard chow diet were used: control group receiving only water, and second an aqueous solution order to provide dose 10 mg/kg/day each mouse. This treatment was maintained for weeks. At moment sacrifice, blood drawn heart removed. Plasma lipids, apolipoproteins monocyte Mac-1 expression assayed as well aortic atherosclerotic areas both...
Conjugated linoleic acids (CLA) affect atherogenesis, but mechanisms are not well understood. We explored how two isomers of CLA, cis9, trans11-CLA and trans10, cis12-CLA, affected lipid glucose metabolism, as hepatic protein expression, in apolipoprotein E knockout mice. After 12 wk intervention, plasma triglyceride, NEFA, concentrations were significantly higher the cis12-CLA group, whereas glucose, insulin lower compared with control mice consuming acid. Proteomics identified significant...
Abstract Organotypic liver culture models for hepatotoxicity studies that mimic in vivo hepatic functionality could help facilitate improved strategies early safety risk assessment during drug development. Interspecies differences sensitivity and mechanistic profiles, low predictive capacity limitations of conventional monocultures human hepatocytes, with high attrition rates remain major challenges. Herein, we show stable, cell-type specific phenotype/cellular polarity differentiated...
We assessed the effects of Picual and Arbequina olive oil, rich poor in polyphenols, respectively, on plasma lipid glucose metabolism, hepatic fat content, proteome female Apoe-/- mice. Both oils increased content adipophilin levels (p < 0.05), though oil significantly decreased triglycerides 0.05). Proteomics identified a range antioxidant enzymes that were differentially regulated by both as compared with palm oil. found clear association between consumption differential regulation betaine...
In human reproduction, hyperhomocysteinemia has been reported as a risk factor for early pregnancy loss and congenital birth defects. Hyperhomocysteinemia is also recognized cause of maternal obstetric complications such preeclampsia. The role plasma in female fertility was examined using cystathionine beta synthase knockout (cbs KO) mice. Cbs KO females were infertile, showed alterations the estrus cycle an increased progesterone response during pseudo-pregnancy induction. Both cbs ovaries...
Aim: Research suggests that sex may condition atherosclerosis development against different genetic backgrounds. This study addresses the hypothesis this effect would be exerted by changes in apolipoproteins present high-density lipoproteins.Methods: ApoE-deficient mice of both sexes with Ola 129 and C57BL/6J backgrounds were fed a chow diet for 14 weeks. At end dietary intervention, atherosclerotic lesions, apolipoproteins, lipid metabolism, inflammation paraoxonase assessed.Results:...
Background and Aims The present study was designed to verify the influence of acute fat loading on high density lipoprotein (HDL) composition, involvement liver different segments small intestine in changes observed. Methods Results To address these issues, rats were administered a bolus 5-ml extra-virgin olive oil sacrificed 4 8 hours after feeding. In animals, lipoproteins analyzed gene expressions apolipoprotein HDL enzymes assessed duodenum, jejunum, ileum liver. Using this experimental...