A5008532501- Liver Disease Diagnosis and Treatment
- Folate and B Vitamins Research
- Metabolism and Genetic Disorders
- Epigenetics and DNA Methylation
- RNA modifications and cancer
- Diet, Metabolism, and Disease
- Metabolomics and Mass Spectrometry Studies
- Pancreatic function and diabetes
- Cancer, Hypoxia, and Metabolism
- Cancer-related gene regulation
- Sulfur Compounds in Biology
- Metabolism, Diabetes, and Cancer
- Liver Disease and Transplantation
- Amino Acid Enzymes and Metabolism
- Liver physiology and pathology
- Endoplasmic Reticulum Stress and Disease
- Cellular Mechanics and Interactions
- Alcohol Consumption and Health Effects
- Lipid metabolism and biosynthesis
- Biochemical and Molecular Research
- Peroxisome Proliferator-Activated Receptors
- Porphyrin Metabolism and Disorders
- Mitochondrial Function and Pathology
- Nitric Oxide and Endothelin Effects
- Lipid Membrane Structure and Behavior
CIC bioGUNE
2016-2025
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2016-2025
Instituto de Salud Carlos III
2017-2024
Euskadiko Parke Teknologikoa
2012-2023
Cedars-Sinai Medical Center
2014-2023
University of Florida
2023
Medical Research Network
2018-2023
University of Geneva
2021
BioCruces Health research Institute
2020
Osakidetza
2020
Exosomes represent a discrete population of vesicles that are secreted from various cell types to the extracellular media. Their protein and lipid composition consequence sorting events at level multivesicular body, central organelle which integrates endocytic secretory pathways. Characterization exosomes different biological samples has shown presence common as well cell-type specific proteins. Remarkably, content is modified upon pathological or stress conditions. Hepatocytes play role in...
Liver-specific and nonliver-specific methionine adenosyltransferases (MATs) are products of two genes, MAT1A MAT2A , respectively, that catalyze the formation S -adenosylmethionine (AdoMet), principal biological methyl donor. Mature liver expresses whereas is expressed in extrahepatic tissues induced during growth dedifferentiation. To examine influence on hepatic growth, we studied effects a targeted disruption murine gene. mRNA protein levels were absent homozygous knockout mice. At 3...
Although Schwann cell myelin breakdown is the universal outcome of a remarkably wide range conditions that cause disease or injury to peripheral nerves, cellular and molecular mechanisms make cell–mediated digestion possible have not been established. We report cells degrade after by novel form selective autophagy, myelinophagy. Autophagy was up-regulated myelinating nerve injury, debris present in autophagosomes, pharmacological genetic inhibition autophagy impaired clearance. Myelinophagy...
Glycine N-methyltransferase (GNMT) is the main enzyme responsible for catabolism of excess hepatic S-adenosylmethionine (SAMe). GNMT absent in hepatocellular carcinoma (HCC), messenger RNA (mRNA) levels are significantly lower livers patients at risk developing HCC, and has been proposed to be a tumor-susceptibility gene liver cancer. The identification several children with disease as having mutations further suggests that this plays an important role function. In current study we studied...
COVID-19 is a systemic infection that exerts significant impact on the metabolism. Yet, there little information how SARS-CoV-2 affects Using NMR spectroscopy, we measured metabolomic and lipidomic serum profile from 263 (training cohort) + 135 (validation symptomatic patients hospitalized after positive PCR testing for infection. We also established profiles of 280 persons collected before coronavirus pandemic started. Principal-component analysis discriminated both cohorts, highlighting...
We have measured the activity S -adenosyl-L-methio-nine synthetase in liver biopsies from a group of controls (n = 17) and 26 cirrhotics (12 alcoholic 14 posthepatitic). The this enzyme was markedly reduced (285 ± 32 pmoles per min mg protein) when compared with that observed (505 37 protein). No differences -adenosyl-L-methionine between both groups cirrhotics. Similarly, marked reduction phospholipid methyltransferase also same (105 12 control subjects (241 13 Again, no difference These...
In mammals, methionine metabolism occurs mainly in the liver via adenosyltransferase-catalyzed conversion to S-adenosylmethionine. Of two genes that encode adenosyltransferase(MAT1Aand MAT2A), MAT1A is expressed adult whereas MAT2A all extrahepatic tissues. Mice lacking have reduced hepatic S-adenosylmethionine content and hyperplasia spontaneously develop nonalcoholic steatohepatitis. this study, we examined whether chronic deficiency generates oxidative stress predisposes injury malignant...
This research analyzes some aspects of the relationship between gene expression, function, and annotation. Many recent studies are implicitly based on assumption that products biologically functionally related would maintain this similarity both in their expression profiles as well Gene Ontology (GO) We analyze how accurate proves to be using real publicly available data. also aim validate a measure semantic for GO use Pearson correlation coefficient its absolute value products. explore...
This study identifies and partially characterizes an insulin-sensitive glycophospholipid in H35 hepatoma cells.The incorporation of ['H]glucosamine into cell lipids was investigated.A major labeled lipid purified by sequential thin layer chromatography using first acid followed a basic solvent system.After hydrochloric hydrolysis sugar analysis chromatography, 80% the radioactivity found to comigrate with glucosamine.H35 cells were prelabeled for either 4 or 24 h treated insulin causing...
Background Obesity modulates inflammation and activation of immune pathways which can lead to liver complications. We aimed at identifying expression patterns inflammatory response genes specifically associated with obesity NASH in the morbidly obese patients. Methodology/Principal Findings Expression 222 was evaluated by quantitative RT-PCR patients histologically normal (n = 6), or severe steatosis without 6) lean controls 5). Hepatic 58 out upregulated The most notable changes occurred...
Our understanding of the mechanisms by which nonalcoholic fatty liver disease (NAFLD) progresses from simple steatosis to steatohepatitis (NASH) is still very limited. Despite growing number studies linking with altered serum metabolite levels, an obstacle development metabolome-based NAFLD predictors has been lack large cohort data biopsy-proven patients matched for key metabolic features such as obesity. We studied 467 biopsied individuals normal histology (n = 90) or diagnosed (steatosis,...