A5054711971- Viral Infections and Vectors
- Vector-borne infectious diseases
- Cancer-related gene regulation
- RNA modifications and cancer
- Single-cell and spatial transcriptomics
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Vector-Borne Animal Diseases
- MicroRNA in disease regulation
- RNA Research and Splicing
- Circadian rhythm and melatonin
- Neurogenesis and neuroplasticity mechanisms
- Regional Development and Environment
- Signaling Pathways in Disease
- Gender Studies in Language
- Cancer, Hypoxia, and Metabolism
- Animal Genetics and Reproduction
- Immune Response and Inflammation
- Amino Acid Enzymes and Metabolism
- Immunotherapy and Immune Responses
- Nerve injury and regeneration
- Histone Deacetylase Inhibitors Research
- Chemical Reactions and Isotopes
- Adipose Tissue and Metabolism
CIC bioGUNE
2010-2025
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2014-2020
Instituto de Salud Carlos III
2020
Johns Hopkins University
2009
Although Schwann cell myelin breakdown is the universal outcome of a remarkably wide range conditions that cause disease or injury to peripheral nerves, cellular and molecular mechanisms make cell–mediated digestion possible have not been established. We report cells degrade after by novel form selective autophagy, myelinophagy. Autophagy was up-regulated myelinating nerve injury, debris present in autophagosomes, pharmacological genetic inhibition autophagy impaired clearance. Myelinophagy...
Cell adaptation to changes in oxygen (O 2 ) availability is controlled by two subfamilies of O -dependent enzymes: the hypoxia inducible factor (HIF)–prolyl and asparaginyl hydroxylases [prolyl domain (PHDs) inhibiting HIF (FIH)]. These sensors regulate activity HIF, a transcriptional complex central homeostasis. In well oxygenated cells, PHDs hydroxylate HIFα subunits, thereby targeting them for proteasomal degradation. contrast, acute inhibits PHDs, leading stabilisation. However, here we...
The initial exposure to pathogens and commensals confers innate immune cells the capacity respond distinctively upon a second stimulus. This training might play key functions in developing an adequate response continuous bacteria. However, mechanisms involved induction of trained immunity by remain mostly unexplored. A. muciniphila represents attractive candidate study promotion these long-term responses. Here, we show that priming macrophages with live enhances bacterial intracellular...
Cancer cells can develop a strong addiction to discrete molecular regulators, which control the aberrant gene expression programs that drive and maintain cancer phenotype. Here, we report identification of RNA-binding protein HuR/ELAVL1 as central oncogenic driver for malignant peripheral nerve sheath tumors (MPNSTs), are highly aggressive sarcomas originate from Schwann cell lineage. HuR was found be elevated bound multitude cancer-associated transcripts in human MPNST samples. Accordingly,...
Sex determination mechanisms are thought to evolve rapidly and show little conservation among different animal species. For example, the critical gene on Y chromosome, <i>SRY</i>, that determines sex in most mammals, is not found other animals. However, a related Sox domain transcription factor, <i>SOX9</i>, also required for testis development mammals exhibits male-specific gonad expression vertebrate Previously, we <i>Drosophila</i> orthologue of...
Thymocyte differentiation is a complex process involving well-defined sequential developmental stages that ultimately result in the generation of mature T-cells. In this study, we analyzed DNA methylation and gene expression profiles at successive human thymus stages. Gain loss occurred during thymocyte differentiation, but demethylation was much more frequent than de novo strongly correlated with expression. These changes took place CpG-poor regions were closely associated T-cell TCR...
RNA modifications, including N-7-methylguanosine (m7G), are pivotal in governing stability and gene expression regulation. The accurate detection of internal m7G modifications is paramount significance, given recent associations between altered deposition elevated the methyltransferase METTL1 various human cancers. development robust techniques has posed a significant challenge field epitranscriptomics. In this study, we introduce two methodologies for global identification RNA. We...
Recent studies have increasingly focused on the role of fungi, including Candida albicans , in carcinogenesis. Since C. is a component human microbiota, particularly skin, we investigated its effect phenotype and signalling pathways melanoma cells. Assays for migration, adhesion, angiogenesis, hepatic metastasis showed that promotes more malignant At transcriptomic level, increased expression VEGF ( Vegfa ), genes associated with MAPK HIF-1 pathways, aerobic glycolysis. Further vitro...
Myelination is essential for neuronal function and health. In peripheral nerves, >100 causative mutations have been identified that cause Charcot-Marie-Tooth disease, a disorder can affect myelin sheaths. Among these, number of are related to targets the posttranslational modification neddylation, although how these lead defects unclear. Here, we demonstrate inhibiting neddylation leads notable absence axonal loss both in developing regenerating mouse nerves. Our data indicate exerts...
Cerebrospinal fluid (CSF) microRNAs (miRNAs) have emerged as potential biomarkers for minimally invasive diagnosis of central nervous system malignancies.However, despite significant advances in recent years, this field still suffers from poor data reproducibility.This is especially true cases infants, considered a new subject group.Implementing efficient methods to study miRNAs clinically realistic CSF volumes necessary the identification biomarkers.Methods: We compared six protocols...
Abstract Prostate cancer exhibits high prevalence and accounts for a number of cancer-related deaths. The discovery characterization molecular determinants aggressive prostate represents an active area research. Immediate Early Response (IER) family genes, which regulate Protein Phosphatase 2A (PP2A) activity, has emerged among the factors that influence biology. Here, we show less studied member this family, 5 like (IER5L), is upregulated in cancer. Interestingly, upregulation IER5L...
Macrophages are cells of the innate immune system with ability to phagocytose and induce a global pattern responses that depend on several signaling pathways. We have determined biosignature murine bone marrow-derived macrophages human blood monocytes using transcriptomic proteomic approaches. identified common genes transcriptionally regulated overall indicate response B. burgdorferi involves interaction spirochetal antigens inflammatory pathways corresponding primary (triggered by...