A5027158354- Liver Disease Diagnosis and Treatment
- Metabolomics and Mass Spectrometry Studies
- Diet and metabolism studies
- Diet, Metabolism, and Disease
- Long-Term Effects of COVID-19
- COVID-19 Clinical Research Studies
- Reproductive Biology and Fertility
- Sperm and Testicular Function
- Gut microbiota and health
- Neonatal Health and Biochemistry
- SARS-CoV-2 and COVID-19 Research
- Liver physiology and pathology
- Endoplasmic Reticulum Stress and Disease
- Biosensors and Analytical Detection
- Diabetes and associated disorders
- SARS-CoV-2 detection and testing
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Folate and B Vitamins Research
- Drug-Induced Hepatotoxicity and Protection
- Liver Disease and Transplantation
- Heme Oxygenase-1 and Carbon Monoxide
- Aluminum toxicity and tolerance in plants and animals
- Bioinformatics and Genomic Networks
- Corporate Identity and Reputation
- Advanced Causal Inference Techniques
CIC bioGUNE
2020-2025
Centro de Investigación Biomédica en Red de Enfermedades Hepáticas y Digestivas
2020-2024
Euskadiko Parke Teknologikoa
2021-2024
University of the Basque Country
2019-2021
COVID-19 is a systemic infection that exerts significant impact on the metabolism. Yet, there little information how SARS-CoV-2 affects Using NMR spectroscopy, we measured metabolomic and lipidomic serum profile from 263 (training cohort) + 135 (validation symptomatic patients hospitalized after positive PCR testing for infection. We also established profiles of 280 persons collected before coronavirus pandemic started. Principal-component analysis discriminated both cohorts, highlighting...
We previously identified subsets of patients with NAFLD different metabolic phenotypes. Here we align metabolomic signatures cardiovascular disease (CVD) and genetic risk factors.
Quantitative plasma lipoprotein and metabolite profiles were measured on an autonomous community of the Basque Country (Spain) cohort consisting hospitalized COVID-19 patients (n = 72) a matched control group 75) Western Australian (WA) 17) SARS-CoV-2 positives 20) healthy controls using 600 MHz 1H nuclear magnetic resonance (NMR) spectroscopy. Spanish samples in two laboratories one-dimensional (1D) solvent-suppressed T2-filtered methods with vitro diagnostic quantification lipoproteins...
Hepatic ischemia-reperfusion injury (IRI) is the leading cause of early posttransplantation organ failure as mitochondrial respiration and ATP production are affected. A shortage donors has extended liver donor criteria, including aged or steatotic livers, which more susceptible to IRI. Given lack an effective treatment extensive transplantation waitlist, we aimed at characterizing effects accelerated activity by silencing methylation-controlled J protein (MCJ) in three preclinical models...
COVID‐19 is a systemic infectious disease that may affect many organs, accompanied by measurable metabolic dysregulation. The also associated with significant mortality, particularly among the elderly, patients comorbidities, and solid organ transplant recipients. Yet, largest segment of patient population asymptomatic, most other develop mild to moderate symptoms after SARS‐CoV‐2 infection. Here, we have used NMR metabolomics characterize plasma samples from cohort abovementioned group ( n...
Neddylation is a druggable and reversible ubiquitin-like post-translational modification upregulated in many diseases, including liver fibrosis, hepatocellular carcinoma, more recently, non-alcoholic fatty disease (NAFLD). Herein, we propose to address the effects of neddylation inhibition underlying mechanisms pre-clinical models NAFLD.Hepatic measured by immunohistochemical analysis NEDD8 serum levels ELISA assay were evaluated NAFLD clinical samples. The using pharmacological small...
Improved methods are required for investigating the systemic metabolic effects of SARS-CoV-2 infection and patient stratification precision treatment. We aimed to develop an effective method using lipid profiles discriminating between infection, healthy controls, non-SARS-CoV-2 respiratory infections. Targeted liquid chromatography–mass spectrometry profiling was performed on discovery (20 SARS-CoV-2-positive; 37 controls; 22 COVID-19 symptoms but SARS-CoV-2negative) validation (312 100...
Abstract Background Metabolic syndrome (MetS) is a multimorbid long-term condition without consensual medical definition and diagnostic based on compatible symptomatology. Here we have investigated the molecular signature of MetS in urine. Methods We used NMR-based metabolomics to investigate European cohort including urine samples from 11,754 individuals (18–75 years old, 41% females), designed populate all intermediate conditions MetS, subjects any risk factor up with developed (4–5%,...
•CNNM4 acts as a magnesium exporter in the liver. Its upregulation NASH leads to elevated levels serum.•Liver-specific CNNM4 targeting alleviates steatosis, inflammation, and fibrosis preclinical models.•siRNA-mediated downregulation promotes hepatic accumulation reduces endoplasmic reticulum stress.•Silencing enhances microsomal triglyceride transfer protein activity leading VLDL assembly secretion. Background & AimsPerturbations of intracellular (Mg2+) homeostasis have implications for...
In vitro maturation (IVM) of oocytes is clinically used in horses to produce blastocysts but current conditions for are suboptimal. We analyzed the composition equine preovulatory follicular fluid (FF) secretome and tested its effects on meiotic competence gene expression subjected IVM. Preovulatory FF was obtained, concentrated using ultrafiltration with cut-off 10 kDa, stored at −80 °C. The metabolic proteomic analyzed, ultrastructural assessed by cryo-transmission microscopy. Oocytes...
We report the successful development and translation of high-field nuclear magnetic resonance (NMR) based comprehensive lipoprotein analysis to routine benchtop systems. This demonstrates potential reimagine population level cardiovascular disease risk individual screening on blood sampling. Using a quantitative calibration approach, we obtained stable reproducible results from multiple sites, despite reduced spectral dispersion sensitivity at lower field strengths. Our study shows that 25...
Hepatic deletion of methionine adenosyltransferase-1a (Mat1a) in mice reduces S-adenosylmethionine (SAMe), a key methyl donor essential for many biological processes, which promotes the development and progression metabolic dysfunction-associated steatotic liver disease (MASLD). Hyperglycemia reduced MAT1A expression, along with low SAMe levels, are common MASLD patients. This study explores how Mat1a-knockout (KO) hepatocytes respond to prolonged high glucose conditions, focusing on...
Precision medicine requires biomarkers that stratify patients and improve clinical outcomes. Although longitudinal multi-omic analyses provide insights into pathological states, their utility in stratifying healthy individuals remains underexplored. We performed a cross-sectional integrative study of three omic layers, including genomics, urine metabolomics, serum metabolomics/lipoproteomics, on cohort 162 without manifestations. studied each layer separately after integration, concluding...
BACKGROUNDArachidyl amido cholanoic acid (Aramchol) is a potent downregulator of hepatic stearoyl-CoA desaturase 1 (SCD1) protein expression that reduces liver triglycerides and fibrosis in animal models steatohepatitis.In phase IIb clinical trial patients with nonalcoholic steatohepatitis (NASH), 52 wk treatment Aramchol reduced blood levels glycated hemoglobin A1c, an indicator glycemic control. AIMTo assess lipid glucose metabolism mouse hepatocytes NASH model [induced 0.1% methionine...
Abstract There is an ongoing need of developing sensitive and specific methods for the determination SARS-CoV-2 seroconversion. For this purpose, we have developed a multiplexed flow cytometric bead array (C19BA) that allows identification IgG IgM antibodies against three immunogenic proteins simultaneously: spike receptor-binding domain (RBD), protein subunit 1 (S1) nucleoprotein (N). Using different cohorts samples collected before after pandemic, show assay more than ELISAs performed in...
Mesoamerican nephropathy (MeN) is a form of chronic kidney disease found predominantly in young men Mesoamerica. Strenuous agricultural labor consistent risk factor for MeN, but the pathophysiologic mechanism leading to poorly understood. We compared urine metabolome among Nicaragua engaged sugarcane harvest and seed cutting (n = 117), group at high against three referents: Nicaraguans working less strenuous jobs same plantations 78); performing non-agricultural work 102); workers Spain 78)....
There has been an intense focus to uncover the molecular mechanisms by which fasting triggers adaptive cellular responses in major organs of body. Here, we show that mice, hepatic S-adenosylmethionine (SAMe)-the principal methyl donor-acts as a metabolic sensor nutrition fine-tune catabolic-fasting response modulating phosphatidylethanolamine N-methyltransferase (PEMT) activity, endoplasmic reticulum-mitochondria contacts, β-oxidation, and ATP production liver, together with FGF21-mediated...
To ensure biological validity in metabolic phenotyping, findings must be replicated independent sample sets. Targeted workflows have long been heralded as ideal platforms for such validation due to their robust quantitative capability. We evaluated the capability of liquid chromatography-mass spectrometry (LC-MS) assays targeting organic acids and bile validate phenotypes SARS-CoV-2 infection. Two sets were collected: (1) Australia: plasma, positive (n = 20), noninfected healthy controls 22)...
Liver fibrosis is the excessive accumulation of extracellular matrix proteins that occurs in chronic liver disease. Ubiquitination a post-translational modification crucial for plethora physiological processes. Even though ubiquitin system has been implicated several human diseases, role ubiquitination remains poorly understood. Here, multi-omics approaches were used to address this. Untargeted metabolomics showed carbon tetrachloride (CCl4)-induced promotes changes hepatic metabolome,...
Background and Aims The liver plays a central role in all metabolic processes the body. However, precise characterization of metabolism is often obscured by its inherent complexity. Phosphorylated metabolites occupy prominent position anabolic catabolic pathways. Here, we develop 31 P nuclear magnetic resonance (NMR)–based method to study “phosphorome” through simultaneous identification quantification multiple hydrophilic hydrophobic phosphorylated metabolites. Approach Results We applied...
Prostate cancer is the most frequent malignancy in European men and second worldwide. One of major oncogenic events this disease includes amplification transcription factor cMYC. Amplification oncogene chromosome 8q24 occurs concomitantly with copy number increase a subset neighboring genes regulatory elements, but their contribution to pathogenesis poorly understood. Here we show that TRIB1 among robustly upregulated coding within amplicon prostate cancer. Moreover, demonstrate...
After SARS-CoV-2 infection, the molecular phenoreversion of immunological response and its associated metabolic dysregulation are required for a full recovery patient. This process is patient-dependent due to manifold possibilities induced by virus severity, phylogenic evolution vaccination status population. We have here investigated natural history COVID-19 disease at level, characterizing over time in large cohorts hospitalized severe patients (n = 886) non-hospitalized recovered that...
Metabolic syndrome (MetS) is a cluster of medical conditions and risk factors correlating with insulin resistance that increase the developing cardiometabolic health problems. The specific criteria for diagnosing MetS vary among different organizations but are typically based on evaluation abdominal obesity, high blood pressure, hyperglycemia, dyslipidemia. A unique, quantitative independent estimation only biomarkers highly desirable comparison between patients to study individual...