A5049638236- Lipoproteins and Cardiovascular Health
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Cancer, Lipids, and Metabolism
- Lipid metabolism and disorders
- Cholesterol and Lipid Metabolism
- Atherosclerosis and Cardiovascular Diseases
- Nitric Oxide and Endothelin Effects
- Genetic Associations and Epidemiology
- Ion channel regulation and function
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Fatty Acid Research and Health
- Adipokines, Inflammation, and Metabolic Diseases
- Lysosomal Storage Disorders Research
- Allergic Rhinitis and Sensitization
- Neuropeptides and Animal Physiology
- Protease and Inhibitor Mechanisms
- Receptor Mechanisms and Signaling
- Cardiac Ischemia and Reperfusion
- Dermatology and Skin Diseases
- Lipid metabolism and biosynthesis
- Glycosylation and Glycoproteins Research
- Drug Transport and Resistance Mechanisms
- Contact Dermatitis and Allergies
- Hormonal Regulation and Hypertension
Instituto Aragonés de Ciencias de la Salud
2006-2021
Hospital Universitario Miguel Servet
2006-2021
Instituto de Investigación Sanitaria Aragón
2011-2021
Universidad Autónoma de Madrid
1991-2021
Universidad San Jorge
2016
Universidad de Zaragoza
1996-2007
Inserm
2004
Institut Pasteur
2003-2004
Expression of human apolipoprotein (h-apo) A-IV in apoE-deficient (apoE(0)) mice (h-apoA-IV/E(0)) reduces susceptibility to atherosclerosis. Chronic infection mimicked by exposure lipopolysaccharide (LPS) increases the size atherosclerosis lesions apoE(0) mice. Thus, we used h-apoA-IV/E(0) determine whether h-apoA-IV plays a protective role after LPS administration.We injected apoE(0), h-apoA-IV/E(0), and C57Bl/6 (wild-type) intraperitoneally with either or phosphate-buffered saline (PBS)...
Abstract Atherosclerosis is an inflammatory disease in which oxidized low-density lipoprotein (oxLDL) plays important roles. Scavenger receptors (SR) CD36, SR-A, and LOX-1 uptake over 90% of the oxLDL leading to foam cell formation secretion cytokines. To investigate whether interindividual differences macrophage SR gene expression could determine variability response oxLDL, we quantified protein molecules from macrophages isolated 18 volunteer subjects incubated with for 1, 3, 6, h. The...
Background TRPV4 channels are calcium-permeable cation that activated by several physicochemical stimuli. Accordingly, have been implicated in the regulation of osmosensing, mechanotransduction, thermosensation, and epithelial/endothelial barrier functions. Whether is also mechanistically melanoma cell proliferation not clear. Here, we hypothesized expressed human pharmacological activation interferes with proliferation. Methodology/Principal findings functions were studied lines (A375,...
Abstract Myelin deficiency in the central nervous system (CNS) can cause severe disabling conditions. Most of transgenic mice models overexpressing myelin components have limitations for investigators and therapy as they severely alter CNS architecture. It has been postulated that transferrin (Tf) is involved oligodendrocyte (OL) maturation myelinogenesis. Because Tf not an intrinsic constituent, we decided to investigate if its overexpression could impact on myelination process without...
Background KCa3.1 channels are calcium/calmodulin-regulated voltage-independent K+ that produce membrane hyperpolarization and shape Ca2+-signaling thereby physiological functions in epithelia, blood vessels, white red cells. Up-regulation of is evident fibrotic inflamed tissues some tumors rendering the channel a potential drug target. In present study, we searched for novel potent small molecule inhibitors by testing series 20 selected natural synthetic (poly)phenols, benzoic acids,...
A new class of emissive cyclometallated Ir
The role of nitric oxide in the cerebral circulation under basal conditions and after vasodilator stimulation was studied instrumented, conscious goats, by examining action inhibiting endogenous production with N G ‐nitro‐ l ‐arginine methyl ester ( ‐NAME). In 6 unanaesthetized blood flow to one brain hemisphere (electromagnetically measured), systemic arterial pressure heart rate were continuously recorded. ‐NAME (35 mg kg −1 i.v. bolus) decreased resting 43 ± 3%, increased mean 21 2%, 41...
Autosomal dominant hypercholesterolemia (ADH) is frequently caused by functional mutations in the low-density lipoprotein receptor (LDLR) or apolipoprotein B-100 (APOB) genes, but approximately 40% of ADH subjects disclose no such molecular defects, possibly pointing to alternative genetic mechanisms.Our objective was test hypothesis that increased intestinal cholesterol absorption might play a role lipid abnormalities with without identified defects.This cross-sectional study consecutive...
1 Ear (cutaneous) and femoral (deep) arteries from rabbit were perfused at 37°C 24°C (cooling) the production of nitrite, as an index nitric oxide production, was measured under basal conditions cholinergic stimulation. 2 In both types control conditions, nitrite similar 37°C. Compared with significantly lower in ear without endothelium or treated NG-nitro-L-arginine methyl ester (L-NAME, 10−4m) but it those atropine (10−6m). 3 At 37°C, methacholine (10−7-10−5 m) increased arteries; this...
Transferrin (Tf), the iron transport glycoprotein found in biological fluids of vertebrates, is synthesized mainly by hepatocytes. Tf also oligodendrocytes (Ol), and several lines evidence indicate that brain could be involved myelinogenesis. Because postnatally expressed brain, we sought to investigate whether intervene Ol differentiation. For this purpose, analyzed transgenic mice overexpressing complete human gene Ol. We show hTf transgene was only from 5 days postpartum onward. In...
Cenarro A, García-Otín A-L, Tejedor MT, Solanas M, Jarauta E, Junquera C, Ros Mozas P, Puzo J, Pocoví Civeira F. A presumptive new locus for autosomal dominant hypercholesterolemia mapping to 8q24.22. Molecular testing of patients with (ADH) fails detect a causal functional mutation in 15.25% subjects. We studied an ADH pedigree which known ADH-causing genes (LDLR, APOB and PCSK9) were excluded. Genome-wide analysis on 15 family members detected significant association dbSNP RS ID rs965814...
1 The role of nitric oxide in the coronary circulation under basal conditions and when exposed to various vasodilator stimuli was studied instrumented, anaesthetized goats, by examining action inhibiting endogenous production with NG-nitro-l-arginine methyl ester (l-NAME). 2 In 12 left circumflex blood flow (electromagnetically measured), systemic arterial pressure heart rate were continuously recorded. l-NAME (3–4, or 8–10 mg kg−1 injected i.v.) decreased resting 20 28%, increased mean 23...
The role of the endothelium in effects cooling on response to cholinoceptor stimulation rabbit central ear (cutaneous) and femoral (non‐cutaneous) arteries was studied using 2 mm long cylindrical segments. Concentration‐response curves for acetylcholine (10 −9 ‐10 −5 m ), methacholine ) sodium nitroprusside −4 were isometrically recorded under conditions, with without or following pretreatment nitric oxide‐synthesis inhibitor N G ‐nitro‐ l ‐arginine methyl ester ( ‐NAME, 10 −6 ‐3 x at 37°C...
The calcium/calmodulin-gated KCa3.1 channel regulates normal and abnormal mitogenesis by controlling K+-efflux, cell volume membrane hyperpolarization-driven calcium-entry. Recent studies suggest modulation of omega-3 fatty acids as negative modulators impaired functions in the inherited lysosomal storage disorder (LSD), Fabry disease (FD). In first part present study, we characterize murine human fibroblasts test impact on fibroblast proliferation. second, study whether is altered LSDs, FD...
Abstract The unique combination of physical and optical properties silica (core)/gold (shell) nanoparticles (gold nanoshells) makes them especially suitable for biomedicine. Gold nanoshells are used from high‐resolution in vivo imaging to photothermal tumor treatment. Furthermore, their large scattering cross‐section the second biological window (1000–1700 nm) also adequate molecular coherence tomography (OCT). In this work, it is demonstrated that, after functionalization, gold with...