A5033340121- Sirtuins and Resveratrol in Medicine
- PARP inhibition in cancer therapy
- DNA Repair Mechanisms
- Autophagy in Disease and Therapy
- CRISPR and Genetic Engineering
- Calcium signaling and nucleotide metabolism
- Chromosomal and Genetic Variations
- Child and Adolescent Psychosocial and Emotional Development
- Genomics and Chromatin Dynamics
- Attention Deficit Hyperactivity Disorder
- Urological Disorders and Treatments
- Pluripotent Stem Cells Research
- Bladder and Urothelial Cancer Treatments
- Diet and metabolism studies
- Schizophrenia research and treatment
- Genetic diversity and population structure
- Orthoptera Research and Taxonomy
- MicroRNA in disease regulation
- Reproductive Biology and Fertility
- Substance Abuse Treatment and Outcomes
- Genetics, Aging, and Longevity in Model Organisms
- Posttraumatic Stress Disorder Research
- Chemokine receptors and signaling
- NF-κB Signaling Pathways
- Ecology and Vegetation Dynamics Studies
Borough of Manhattan Community College
2024
City University of New York
2020-2024
Rutgers, The State University of New Jersey
2011-2024
Universitat de Barcelona
2019
Robert Wood Johnson University Hospital
2010
Centro de Investigación Biomédica en Red de Salud Mental
2009
Hospital Universitari de Vic
2007
Hospital Universitario 12 De Octubre
1997-1999
Universidad Autónoma de Madrid
1993-1996
The mammalian cytoplasmic protein SirT2 is a member of the Sir2 family NAD + -dependent deacetylases involved in caloric restriction-dependent life span extension. We found that and its yeast counterpart Hst2 have strong preference for histone H4K16Ac their deacetylation activity vitro vivo. pinpointed decrease global levels during cell cycle to G 2 /M transition coincides with localization on chromatin. Mouse embryonic fibroblasts (MEFs) deficient show higher mitosis, contrast normal...
The establishment of the epigenetic mark H4K20me1 (monomethylation H4K20) by PR-Set7 during G 2 /M directly impacts S-phase progression and genome stability. However, mechanisms involved in regulation this event are not well understood. Here we show that SirT2 regulates deposition through deacetylation H4K16Ac (acetylation H4K16) determines levels H4K20me2/3 throughout cell cycle. binds deacetylates at K90, modulating its chromatin localization. Consistently, depletion significantly reduces...
Article25 May 2016Open Access Source DataTransparent process SIRT7 promotes genome integrity and modulates non-homologous end joining DNA repair Berta N Vazquez Department of Genetics, Human Genetics Institute New Jersey, Rutgers University, Piscataway, NJ, USA Search for more papers by this author Joshua K Thackray Nicolas G Simonet Chromatin Biology Laboratory, Cancer Epigenetics Program (PEBC), Bellvitge Biomedical Research (IDIBELL), Barcelona, Spain Noriko Kane-Goldsmith Paloma...
Objective To investigate whether the pathogenesis of rheumatoid arthritis (RA) is associated with functional chemokine receptor CCR5, which primary CC expressed by T cells in synovium, and its nonfunctional receptor, Δ32CCR5, generated homozygous 32-basepair deletion (Δ32) CCR5 gene. Methods The frequency genotype was compared among 673 patients RA, 113 systemic lupus erythematosus (SLE), 815 control subjects. studied polymerase chain reaction amplification region flanking Δ32 (Δ32CCR5)....
HP1 is a structural component of heterochromatin. Mammalian isoforms HP1α, HP1β, and HP1γ play different roles in genome stability, but their precise role heterochromatin structure unclear. Analysis Hp1α−/−, Hp1β−/−, Hp1γ−/− MEFs show that proteins have both redundant unique functions within pericentric (PCH) also act globally throughout the genome. HP1α confines H4K20me3 H3K27me3 to regions PCH, while its absence results global hyper-compaction chromatin associated with specific pattern...
Long interspersed elements-1 (LINE-1, L1) are retrotransposons that hold the capacity of self-propagation in genome with potential mutagenic outcomes. How somatic cells restrict L1 activity and how this process becomes dysfunctional during aging cancer is poorly understood. L1s enriched at lamin-associated domains, heterochromatic regions nuclear periphery. Whether association necessary for their repression has been elusive. Here we show sirtuin family member SIRT7 participates epigenetic...
The maintenance of genomic integrity is crucial to embryonic stem cells (ESC) considering the potential for propagating undesirable mutations resulting somatic and germ cell lineages. Indeed, mouse ESC (mESC) exhibit a significantly lower mutation frequency compared differentiated cells. This could be due more effective elimination genetically damaged via apoptosis, or especially robust, sequence-conserving DNA damage repair mechanisms such as homologous recombination (HR). We used...
Sirtuins are NAD+-dependent deacetylases that facilitate cellular stress response. They include SirT6, which protects genome stability and regulates metabolic homeostasis through gene silencing, whose loss induces an accelerated aging phenotype directly linked to hyperactivation of the NF-κB pathway. Here we show SirT6 binds H3K9me3-specific histone methyltransferase Suv39h1 monoubiquitination conserved cysteines in PRE-SET domain Suv39h1. Following activation signaling is released from IκBα...
Sirtuins are key players of metabolic stress response. Originally described as deacetylases, some sirtuins also exhibit poorly understood mono-adenosine 5'-diphosphate (ADP)-ribosyltransferase (mADPRT) activity. We report that the deacetylase SirT7 is a dual sirtuin, it features auto-mADPRT mADPRT occurs at previously undefined active site, and its abrogation alters chromatin distribution. identify an epigenetic pathway by which ADP-ribosyl-SirT7 recognized ADP-ribose reader mH2A1.1 under...
The Sirtuin family of NAD+-dependent enzymes plays an important role in maintaining genome stability upon stress. Several mammalian Sirtuins have been linked directly or indirectly to the regulation DNA damage during replication through Homologous recombination (HR). one them, SIRT1, is intriguing as it seems a general regulatory response (DDR) that has not yet addressed. SIRT1-deficient cells show impaired DDR reflected decrease repair capacity, increased instability and decreased levels...
Transcriptional regulatory mechanisms likely contribute to the etiology of inflammatory bowel disease (IBD), as genetic variants associated with are disproportionately found at elements. However, transcription factors regulating colonic inflammation unclear. To identify these factors, we mapped epigenomic changes in epithelium upon inflammation. Epigenetic marks transcriptional elements responded dynamically and indicated a shift epithelial factor networks. Active enhancer chromatin...
Abstract B lymphopoiesis is orchestrated by lineage-specific transcription factors. In cell progenitors, lineage commitment mediated Pax5, which commonly mutated in acute lymphoblastic leukemia. Despite its essential role immunity, the mechanisms regulating Pax5 function remain largely unknown. Here, we found that NAD + -dependent enzyme SIRT7 coordinates development through deacetylation of at K198, promotes protein stability and transcriptional activity. Neither K198 deacetylated nor...
p53 is a hallmark tumor suppressor due in part to its role cell cycle progression, DNA damage repair, and cellular apoptosis; protein activity interrelates with the Sirtuin family of proteins, major regulators response metabolic, oxidative, genotoxic stress. In recent years, mammalian 7 (SIRT7) has emerged as pivotal regulator p53, fine-tuning context dependent manner. SIRT7 frequently overexpressed human cancer, yet precise tumorigenesis whether it involves regulation insufficiently...
<i>Objective:</i> Very few studies have assessed substance use in clinical samples of adolescents with eating disorders (ED). This paper reports the prevalence regular or risky (RRSU) and disorder (SUD) ED. <i>Methods:</i> The Teen-Addiction Severity Index (T-ASI), Youth Self-Report (YSR) scale questionnaires were administered to 95 adolescent patients aged 12–17 years who fulfilled DSM-IV-TR diagnostic criteria for anorexia nervosa, bulimia nervosa ED not otherwise...
The presence of H3K9me3 and heterochromatin protein 1 (HP1) are hallmarks conserved in eukaryotes. spreading maintenance is effected by the functional interplay between H3K9me3-specific histone methyltransferase Suv39h1 HP1. This complex mammals because three HP1 isoforms, HP1α, β, γ, thought to play a redundant role Suv39h1-dependent deposition pericentric (PCH). Here, we demonstrate that despite this redundancy, HP1α and, lesser extent, HP1γ have closer link Suv39h1, compared HP1β. γ...
Abstract A study of the variation in pattern and frequency constitutive heterochromatin nucleolar organizing regions X chromosomes male Chorthippus parallelus grasshoppers 25 populations within Iberian peninsula requires us to revise our interpretation biogeography evolutionary history this species. Hybridization between subspecies Cp erythropus , previously only known from Pyrenean cols, is shown extend at least 400 km further into north‐west Spain. novel X‐chromosome variant described that...