A5033539775- Chromosomal and Genetic Variations
- Insect symbiosis and bacterial influences
- Genetic diversity and population structure
- Plant and animal studies
- Insect-Plant Interactions and Control
- Insect and Pesticide Research
- Microfluidic and Bio-sensing Technologies
- Electrowetting and Microfluidic Technologies
- Genomics and Phylogenetic Studies
- Plant Genetic and Mutation Studies
- Advanced biosensing and bioanalysis techniques
- Genomics and Chromatin Dynamics
- Orthoptera Research and Taxonomy
- DNA and Nucleic Acid Chemistry
- Genetic and Environmental Crop Studies
- Metal complexes synthesis and properties
- Innovative Microfluidic and Catalytic Techniques Innovation
- Insect Resistance and Genetics
- Orbital Angular Momentum in Optics
- Insect and Arachnid Ecology and Behavior
- Sunflower and Safflower Cultivation
- Animal Genetics and Reproduction
- Genetic Mapping and Diversity in Plants and Animals
- Evolution and Science Education
- Electrohydrodynamics and Fluid Dynamics
Universidad Autónoma de Madrid
2015-2024
Institut Sophia Agrobiotech
2015
University of East Anglia
1990-1992
Photovoltaic tweezers are a recently proposed technique for manipulation and patterning of micro- nano-objects. It is based in the dielectrophoretic forces associated to electric fields induced by illumination certain ferroelectrics due bulk photovoltaic effect. The has been applied dielectric metal nano-particles. In this work, we report use pattern biological objects on LiNbO3:Fe. Specifically, spores pollen grains their nanometric fragments have trapped patterned. 1D 2D arrangements...
Hybrid zones and the consequences of hybridization have contributed greatly to our understanding evolutionary processes. also provide valuable insight into dynamics symbiosis since each subspecies or species brings its unique microbial symbionts, including germline bacteria such as Wolbachia , hybrid zone. Here, we investigate a natural zone two meadow grasshopper Chorthippus parallelus in Pyrenees Mountains. We set out test whether co-infections B F grasshoppers enabled horizontal transfer...
The process of species formation is characterized by the accumulation multiple reproductive barriers. evolution hybrid male sterility, or Haldane's rule, typically characterizes later stages formation, when isolation strongest. Yet, understanding how quickly barriers evolve and their consequences for maintaining genetic boundaries between emerging remains a challenging task because it requires studying taxa that hybridize in nature. Here, we address these questions using meadow grasshopper...
We investigated the chromosome complement of <i>Muraena Helena </i>L. using C-banding, nucleolar organizer region silver staining, and restriction endonuclease digestion. found a diploid number identical to that previously in other Muraenidae (2n = 42). C-banding revealed presence constitutive heterochromatin centromeres all chromosomes as well most telomeres acrocentric chromosomes. Nucleolar regions were detected only on short arm 7. Digestion with either...
The generation and manipulation of small aqueous droplets is an important issue for nano- biotechnology, particularly, when using microfluidic devices. production very has been frequently carried out by applying intense local electric fields to the fluid, which requires power supplies metallic electrodes. This procedure complicates device reduces its versatility. In this work, we present a novel flexible, best our knowledge, electrodeless optoelectronic method tiny biologically friendly...
The theory of evolution is one the greatest scientific achievements in intellectual history humankind, yet it still contentious within certain social groups. Despite being as robust and evidence-based any other notable theory, some people show a strong reluctance to accept it. In this study, we used Measure Acceptance Theory Evolution (MATE) Knowledge Exam (KEE) questionnaires with university students from four academic degree programs (Chemistry, English, History, Biology) ten universities...
Abstract A study of the variation in pattern and frequency constitutive heterochromatin nucleolar organizing regions X chromosomes male Chorthippus parallelus grasshoppers 25 populations within Iberian peninsula requires us to revise our interpretation biogeography evolutionary history this species. Hybridization between subspecies Cp erythropus , previously only known from Pyrenean cols, is shown extend at least 400 km further into north‐west Spain. novel X‐chromosome variant described that...
The barcoding of the mitochondrial COX1 gene has been instrumental in cataloguing tree life, and providing insights phylogeographic history species. Yet, this strategy encountered difficulties major clades characterized by large genomes, which contain a high frequency nuclear pseudogenes originating from genome (numts). Here, we use meadow grasshopper (Chorthippus parallelus), possesses giant ~13 Gb, to identify genes that are underrepresented as numts, test their informative markers. We...
Abstract We evaluate for the first time effect of Wolbachia infection, involving two different supergroups, on structure and dynamics hybrid zone between subspecies Chorthippus parallelus (Orthoptera) in Pyrenees. infection showed no effects female fecundity or a slight increment females infected by F supergroup, although last case it has to be well established. Cytoplasmic incompatibility ( CI ) is confirmed crosses carried out field individuals from natural population. This , registered as...
Recent advances in next generation sequencing (NGS) have greatly increased our understanding of non-coding tandem repeat (TR) DNA. Here we show how TR DNA can be useful for the study hybrid zones (HZ), as it serves a marker to identify introgression areas where two biological entities come contact. We used Illumina libraries analyse subspecies grasshopper Chorthippus parallelus, which currently form HZ Pyrenees. retrieved total 152 sequences, and fluorescent situ hybridization (FISH) map 77...
The employment of certain DNA-specific fluorescent stains on unhanded and C-banded chromosomes two species grasshoppers shows remarkable differences among C-heterochromatic regions supposed to be similar in their base pair composition, according response the standard fluorescence techniques. possible interspersion opposite DNA pairs these as well role played by proteins chromosome banding are discussed.
Summary The grasshopper Chorthippus parallelus has two quite distinct subspecies, which meet along the Pyrenees forming a hybrid zone. Using silver staining we show that on French side Cp. three nucleolar organizer regions, L 2 , 3 and X chromosomes, while Spanish erythropus only NORs, . Laboratory F1 males reciprocal differences in expression of NORs. When is female parent male progeny four active NORs mitotic cells precipitates meiotic cells, as expected. But when donates with NOR, her...
Chorthippus parallelus (Cpp) and erythropus (Cpe) are two grasshopper subspecies whose distributions overlap in the Pyrenees, where they form a hybrid zone at those points topography their ecological requirements allow. This is consequence of secondary contact between endemic Iberian continental European populations that expanded after most recent glaciations from refugia which had diverged genetically allopatry.The morphological, physiological, genetic behavioral differences these (and...
The potential of photovoltaic tweezers to produce plasmonic platforms for fluorescence enhancement organic or biological molecules is demonstrated. 1D and 2D patterns silver nanoparticles are produced on the surface LiNbO 3 :Fe substrates using this tool, which allows depositing in parallel a large number particles accordance with imposed light profiles. nanoparticle reveal variety features whose resonances cover broad spectral range able efficient enhancement. First, remarkable average...
Wolbachia is a well-known endosymbiotic, strictly cytoplasmic bacterium. It establishes complex cytonuclear relations that are not necessarily deleterious to its host, but often result in reproductive alterations favouring bacterial transmission. Among these alterations, common one the incompatibility (CI) reduces number of descendants certain crosses between infected and non-infected individuals. This CI induced by appears hybrid zone grasshoppers Chorthippus parallelus C. p. erythropus...
The ecological and genetic mechanisms that determine Phasmatodea reproductive biology are poorly understood.The order includes standard sexual species, but also many others display distinct types of parthenogenesis (tychoparthenogenesis, automixis, apomixis, etc.), or both systems facultatively.In a preliminary survey, we analysed Wolbachia Spiroplasma infection in 244 individuals from 28 species 24 genera stick insects by bacterial 16S rRNA gene amplification.Our main aim was to whether...
Abstract Distinct lineages of the grasshopper Chorthippus parallelus (Orthoptera: Acrididae) form well‐known hybrid zones (HZs) both in Pyrenees and Alps mountain ranges South Europe. These HZs represent unique experimental systems to identify “key genes” that maintain genetic boundaries between emerging species. The Iberian endemism C. p. erythropus (Cpe) subspecies (Cpp), widely distributed throughout rest Europe, overlap Pyrenean HZ. Both differ morphologically, as well behavioral,...
The cytoplasmic incompatibility induced by the bacterial endosymbiont <i>Wolbachia</i> is attributed to chromatin modification in sperm of infected individuals and only ‘rescued’ females after fertilization. <i>Chorthippus parallelus</i> a grasshopper with 2 subspecies that form hybrid zone Pyrenees which this <i>Wolbachia</i>-generated has recently been described. analysis certain cytogenetic traits (sex chromosome-linked heterochromatic bands, nucleolar...
Photovoltaic optoelectronic tweezers are a useful platform with many applications in optical manipulation and nanotechnology. They based on electrical forces associated the bulk photovoltaic effect presented by certain ferroelectric crystals, such as Fe doped lithium niobate. This technique has experienced huge developments recent years, although its use biology biomedicine is still scarce. Recently, novel strategy been reported that extends capabilities to of polar droplets, water aqueous...
Summary Two grasshopper subspecies, Chorthippus parallelus (Cpp) and erythropus (Cpe), meet along the Pyrenees where they hybridize produce a hybrid zone. A contact zone located in Col de Portalet has been analyzed for distribution of chromosome markers on sex (X) pure populations. C‐banding allowed us to distinguish both subspecific forms recombinant forms, demonstrate their different frequencies through Interestingly, distal C‐band (P) X that characterizes Cpp individuals occurs at very...