Limited proteolysis of phosphocellulose-purified tubulin with subtilisin resulted in cleavage both alpha and beta subunits, the formation two major fragments (S alpha, S beta, 48 kDa) a small peptide (4 containing carboxyl-terminal region tubulin. Interestingly, cleaved under present conditions showed an increased ability to assemble into large polymers absence MAPs that do not promote assembly undigested tubulin--i.e., low magnesium concentrations taxol polyalcohols. The critical for...

The aim of this study was to compare the rate sperm DNA fragmentation (rSDF; increase SDF over time) in fresh and gradient isolated frozen–thawed semen samples from male donors proven fertility. assessed two obtained same fifteen after 0.5, 1.5, 4.5, 6, 24, 48, 72 h incubation a humidified atmosphere 5% CO2 air at 37°C. Analysis performed based on chromatin dispersion patterns evaluated using Halosperm® kit. No significant differences were when gradient-isolated spermatozoa compared...

We have studied the distribution of potentially active genes on human chromosomes, using two methods: DNAse I hypersensitivity and restriction enzyme – nick translation with enzymes sensitive to methylation CpG doublets. is known be associated genes, and, when reaction detected by "in situ" translation, produces an R-banding pattern. Digestion chromosomes HpaII or CfoI, both which should preferentially cut unmethylated sequences in islands majority also patterns. Deviations are attributable...

The capacity for self-polymerization and shape of the tubulin polymers assembled after digestion with trypsin, Pronase, chymotrypsin, subtilisin, Staphylococcus aureus proteinase V8 K were investigated. Digestion Pronase or chymotrypsin resulted in a decrease ability self-assembly, whereas limited proteolysis S. an increase such ability. varied from typical microtubules (after treatment trypsin Pronase) to sheets chymotrypsin) hooked constant polarity subtilisin) disappearance defined K)....

Abstract We evaluate for the first time effect of Wolbachia infection, involving two different supergroups, on structure and dynamics hybrid zone between subspecies Chorthippus parallelus (Orthoptera) in Pyrenees. infection showed no effects female fecundity or a slight increment females infected by F supergroup, although last case it has to be well established. Cytoplasmic incompatibility ( CI ) is confirmed crosses carried out field individuals from natural population. This , registered as...

The behaviour of two chromosome structures in silver-stained chromosomes was analyzed through the first meiotic division spermatocytes acridoid species Arcyptera fusca. Results showed that at diakinesis kinetochores and chromatid cores are individualized while they associate bivalents metaphase I; only distal core spots sex chromosome. Metaphase I is characterized by morphological localization changes both which define onset anaphase I. These autosomes allow us to distinguish among three...

El fenómeno de la inmigración ha supuesto un impacto en atención a salud población.En España población inmigrante representa hoy día, aproximadamente el 8% su total.Esta mayoría procede países baja renta, y procedencia distribución es diversa.La inmigrada se caracteriza por ser joven, sana, con capacidad adaptación los cambios, pero sus condiciones sociales, económicas laborales, frecuencia son precarias favorecen vulnaribilidad enfermedad.A pesar que número inmigrantes sexo varón...

Sperm quality was assessed in normozoospermic human (n = 10) and Spanish breed stallion after sperm fractionation during ejaculation. The first ejaculated fraction separated from the second. A third sample reconstituted using equivalent proportion of both fractions (RAW). Fraction 1, 2 RAW semen were incubated for 30 min at 37°C to homogenise impact iatrogenic damage between species. concentration, motility DNA each semen. results showed two important facts: (i) spermatozoa confined 1...

An endemic Puerto Rican Elaterid, Pyrophorus luminosus III., has 17 chromosomes in the male, meiotic karyoformula being 7 + X 1 2 Y, or as expressed structurally, p neoXneoY . is heteropycnotic prophase and differentially argentophilous from to metaphase II. The parachute of a modified shape only slightly stained by AgNO 3 Rattenbury's nucleolus staining method.Key words: Elateridae, Pyrophorini, sex chromosomes, trivalent.