ABSTRACT The biosynthetic gene cluster for the aromatic polyketide steffimycin of anthracycline family has been cloned and characterized from “ Streptomyces steffisburgensis ” NRRL 3193. Sequence analysis a 42.8-kbp DNA region revealed presence 36 open reading frames (ORFs) (one them incomplete), 24 which, spanning 26.5 kb, are probably involved in biosynthesis. They code all activities required biosynthesis, tailoring, regulation, resistance but show no evidence genes l -rhamnose...

Expression of the steffimycin gene cluster in Steptomyces albus combination with plasmids directing biosynthesis different neutral and branched-chain deoxyhexoses led to identification twelve new glycosylated derivatives degrees decoration tetracyclic core. These experiments demonstrate flexibility L-rhamnosyltransferase StfG for recognition a variety D- L-deoxyhexoses, harboring deoxygenation as 2-deoxyhexoses, 2,6-deoxyhexoses, 2,3,6-deoxyhexoses, their attachment...

Background Bacterial two-component signal transduction regulatory systems are the major set of signalling proteins frequently mediating responses to changes in environment. They typically consist a sensor, membrane-associated histidine kinase and cytoplasmic response regulator. The sensor detects environmental or stress, whereas protein controls cellular usually by gene transcription modulation. Methodology/PrincipalFindings Streptomyces coelicolor two genes operon SCO5784-SCO5785 encodes...

Background Misfolded proteins accumulating outside the bacterial cytoplasmic membrane can interfere with secretory machinery, hence existence of quality factors to eliminate these misfolded is capital importance in bacteria that are efficient producers proteins. These normally use a specific two-component system respond stress produced by accumulation proteins, activating expression HtrA-like proteases specifically incorrectly folded Methodology/Principal Findings Overproduction...

Streptomyces lividans has demonstrated its value as an efficient host for protein production due to ability secrete functional proteins directly the media. Secretory that use major Sec route need be properly folded outside cell, whereas secretory using Tat appear cell correctly folded. This feature makes system very attractive of natural or engineered proteins. S. cells are known respond differently overproduction and secretion versus Increased understanding impact through can obtained by a...

BACKGROUND Extracellular protein production by Gram-positive bacteria, such as Streptomyces, may be complementary to current established processes. The performance of a Streptomyces lividans mutant strain, deficient in the major signal peptidase (SipY) is investigated for proteins secreted via secondary Tat pathway. RESULTS SipY strain has shown advantages over wild type terms extracellular productivity, specific activity and rheological behaviour. Two operational modes, batch fed-batch,...

Streptomyces lividans is considered an efficient host for the secretory production of homologous and heterologous proteins. To identify possible bottlenecks in protein process, a comparative transcriptomic approach was adopted to study cellular responses during overproduction Sec-dependent model (alpha-amylase) Tat-dependent (agarase) lividans. The proteins via Sec or Tat route S. does elicit different major cell response bacterium. stringent bacterial nutrients' depletion, which naturally...

Overproduction of Sec-proteins in S. lividans accumulates misfolded proteins outside the cytoplasmic membrane where accumulated interfere with correct functioning secretion machinery and cell functionality, triggering expression a CssRS two-component system which regulates degradation protein, so-called stress response. Optimization secretory protein production via Sec route requires identification characterisation quality factors involved this process. The phosphorylated regulator (CssR)...

Biological communities present in soil are essential to sustainable and productive agricultural practices; however, an accurate determination of the ecological status soils remains date elusive task. An ideal indicator should be pervasive, play a relevant role ecosystem, show rapid proportional answer external perturbations easily economically measurable. Rhizobacteria major determining properties, becoming attractive candidate for detection indicators. The application massive sequencing...

Streptomyces lividans is an appealing host for the production of proteins biotechnological interest due to its relaxed exogenous DNA restriction system and ability secrete directly medium through major Sec or minor Tat routes. Often, protein secretion displays non-uniform time-dependent patterns. Understanding associated metabolic changes a crucial step engineer production. Dynamic Flux Balance Analysis (DFBA) allows study interactions between modelled organism environment over time....

Bacterial secretory proteins often require the formation of disulphide bonds outside cell to acquire an active conformation. Thiol-disulphide oxidoreductases are enzymes that catalyse bonds. The bacterium Streptomyces lividans is a well-known host for efficient secretion overproduced homologous and heterologous industrial application. Therefore, correct conformation these extracellular great importance when engineering overproduction.We have identified four acting thiol-disulphide (TDORs) in...

Some bacterial peptidyl-prolyl cis/trans isomerases (PPIases) are involved in secretory protein folding after the translocation step. Streptomyces lividans has been used as a host for engineering extracellular overproduction of homologous and heterologous proteins industrial applications. Although mechanisms governing major pathway (Sec route) minor (Tat reasonably well described, function responsible is not characterized yet. We have Tat-dependent S. FK506-binding protein-like lipoprotein...