A5103185327- Biochemical and Molecular Research
- Plant tissue culture and regeneration
- Adenosine and Purinergic Signaling
- Calcium signaling and nucleotide metabolism
- Epigenetics and DNA Methylation
- Amino Acid Enzymes and Metabolism
- Polyamine Metabolism and Applications
- Lanthanide and Transition Metal Complexes
- Radioactive element chemistry and processing
- Antimicrobial Peptides and Activities
- Enzyme Structure and Function
- Plant nutrient uptake and metabolism
- Crystallography and molecular interactions
- Transgenic Plants and Applications
Palacký University Olomouc
2021-2025
Centre of the Region Haná for Biotechnical and Agricultural Research
2020
Abstract Adenosine undergoes ATP-dependent phosphorylation catalyzed by adenosine kinase (ADK). In plants, ADK also phosphorylates cytokinin ribosides, transport forms of the hormone. Here, we investigated substrate preferences, oligomeric states and structures ADKs from moss (Physcomitrella patens) maize (Zea mays) alongside metabolomic phenotypic analyses. We showed that dexamethasone-inducible ZmADK overexpressor lines in Arabidopsis can benefit a higher number lateral roots larger root...
SUMMARY Polyamines such as spermidine and spermine are essential regulators of cell growth, differentiation, maintenance ion balance abiotic stress tolerance. Their levels controlled by the spermidine/spermine N 1 ‐acetyltransferase (SSAT) via acetylation to promote either their degradation or export outside shown in mammals. Plant genomes contain at least one gene coding for SSAT (also named NATA ‐AcetylTransferase Activity). Combining kinetics, HPLC‐MS crystallography, we show that three...
Antimicrobial peptides play a crucial role in the innate immune system of multicellular organisms. LL-37 is only known member human cathelicidin family. As well as possessing antibacterial properties, it actively involved various physiological responses eukaryotic cells. Accordingly, there considerable interest large-scale, low-cost, and microbial endotoxin-free production recombinant for pharmaceutical applications. heterologous expression biofactory, we have previously obtained homologous...
Cells save their energy during nitrogen starvation by selective autophagy of ribosomes and degradation RNA to ribonucleotides nucleosides. Nucleosides are hydrolyzed nucleoside N-ribohydrolases (nucleosidases, NRHs). Subclass I NRHs preferentially hydrolyzes the purine ribosides while subclass II is more active towards uridine xanthosine. Here, we performed a crystallographic kinetic study shed light on preferences among plant followed in vivo metabolomic phenotyping analyses reveal...
Adenosine undergoes ATP-dependent phosphorylation catalyzed by adenosine kinase (ADK). In plants, ADK also phosphorylates cytokinin ribosides, transport forms of the hormone. Here, we investigated substrate preferences, oligomeric states and structures ADKs from moss (Physcomitrella patens) maize (Zea mays) alongside metabolomic phenotypic analyses. We showed that dexamethasone-inducible ZmADK overexpressor lines in Arabidopsis can benefit a higher number lateral roots larger root areas...