A5040595077- Plant Reproductive Biology
- Plant Molecular Biology Research
- Cellular transport and secretion
- Sport and Mega-Event Impacts
- Sports, Gender, and Society
- Photosynthetic Processes and Mechanisms
- Polysaccharides and Plant Cell Walls
- Plant nutrient uptake and metabolism
- Australian History and Society
- Microtubule and mitosis dynamics
- Legume Nitrogen Fixing Symbiosis
- Plant tissue culture and regeneration
- Educator Training and Historical Pedagogy
- Protein Kinase Regulation and GTPase Signaling
- Glycosylation and Glycoproteins Research
- Retinal Development and Disorders
- Higher Education Learning Practices
- Streptococcal Infections and Treatments
- Skin and Cellular Biology Research
- Canadian Identity and History
- Biofuel production and bioconversion
- Computational Physics and Python Applications
- Evaluation of Teaching Practices
- Antimicrobial Resistance in Staphylococcus
- Plant-Microbe Interactions and Immunity
University of Michigan–Ann Arbor
2012-2023
Macquarie University
2014-2021
South China Agricultural University
2021
Sandia National Laboratories California
2019
Stony Brook University
2015
Stony Brook Medicine
2015
UNSW Sydney
2012-2014
Institut thématique Génétique, génomique et bioinformatique
2012
Donald Danforth Plant Science Center
2003-2008
Washington University in St. Louis
2003-2008
Two endosome populations involved in recycling of membranes and receptors to the plasma membrane have been described, early endosome. However, this distinction is mainly based on flow cargo molecules spatial distribution these within cell. To get insights into organization pathway, we studied Rab4, Rab5, Rab11, three regulatory components transport machinery. Following transferrin as molecule GFP-tagged Rab proteins could show that moves through distinct domains endosomes. These are occupied...
Abstract Small GTP-binding proteins regulate diverse processes in eukaryotic cells such as signal transduction, cell proliferation, cytoskeletal organization, and intracellular membrane trafficking. These function molecular switches that cycle between “active” “inactive” states, this is linked to the binding hydrolysis of GTP. The Arabidopsis genome contains 93 genes encode small protein homologs. Phylogenetic analysis these shows plants contain Rab, Rho, Arf, Ran GTPases, but no Ras...
Auxin transport, which is mediated by specialized influx and efflux carriers, plays a major role in many aspects of plant growth development. AUXIN1 (AUX1) has been demonstrated to encode high-affinity auxin carrier. In Arabidopsis thaliana, AUX1 belongs small multigene family comprising four highly conserved genes (i.e., LIKE [LAX] LAX1, LAX2, LAX3). We report that all members this AUX/LAX display uptake functions. Despite the conservation their biochemical function, AUX1, LAX3 have...
The trans Golgi network (TGN) of plant cells sorts and packages products into secretory (SV) clathrin‐coated (CCV) vesicles. We have analyzed TGN cisternae in Arabidopsis root meristem by cell fractionation electron microscopy/tomography to establish reliable criteria for identifying cells, define their functional attributes. Transformation a cisterna Golgi‐associated begins with cisternal peeling, the formation SV buds outside plane 30–35% reduction membrane area. Free compartments are...
Roots hairs are cylindrical extensions of root epidermal cells that important for acquisition nutrients, microbe interactions, and plant anchorage. The molecular mechanisms involved in the specification, differentiation, physiology Arabidopsis reviewed here. Root hair specification is determined by position-dependent signaling feedback loops causing differential accumulation a WD-bHLH-Myb transcriptional complex. initiation dependent on RHD6 bHLH gene family auxin to define site outgrowth....
Rab5 regulates endocytic membrane traffic by specifically recruiting cytosolic effector proteins to their site of action on early endosomal membranes. We have characterized a new complex involved in fusion events. This includes novel protein, Rabenosyn-5, which, like the previously endosome antigen 1 (EEA1), contains an FYVE finger domain and is recruited phosphatidylinositol-3-kinase–dependent fashion endosomes. Rabenosyn-5 complexed Sec1-like protein hVPS45. hVPS45 does not interact...
The RabA4b GTPase labels a novel, trans-Golgi network compartment displaying developmentally regulated polar distribution in growing Arabidopsis thaliana root hair cells. GTP bound selectively recruits the plant phosphatidylinositol 4-OH kinase, PI-4Kβ1, but not members of other PI-4K families. PI-4Kβ1 colocalizes with on tip-localized membranes hairs, and mutant plants which both -4Kβ2 genes are disrupted display aberrant morphologies. interacts through novel homology domain, specific to...
Spatial and temporal control of cell wall deposition plays a unique critical role during growth development in plants. To characterize membrane trafficking pathways involved these processes, we have examined the function plant Rab GTPase, RabA4b, polarized expansion developing root hair cells. Whereas small fraction RabA4b cofractionated with Golgi marker proteins, majority this protein labeled compartment that did not cofractionate previously characterized trans-Golgi network syntaxin...
The dynamic activity of tip-localized filamentous actin (F-actin) in pollen tubes is controlled by counteracting RIC4 and RIC3 pathways downstream the ROP1 guanosine triphosphatase promoting assembly disassembly, respectively. We show here that activation required for both polar accumulation exocytosis vesicles at plasma membrane apex. apical exocytic oscillated phase with, but slightly behind, was enhanced overexpression RIC4. However, inhibited exocytosis, this inhibition could be...
Abstract In yeast and mammals, the AAA ATPase Vps4p/SKD1 (for Vacuolar protein sorting 4/SUPPRESSOR OF K+ TRANSPORT GROWTH DEFECT1) is required for endosomal of secretory endocytic cargo. We identified a VPS4/SKD1 homolog in Arabidopsis thaliana, which localizes to cytoplasm multivesicular endosomes. addition, green fluorescent protein–SKD1 colocalizes on bodies with fusion Rab GTPases, such as ARA6/RabF1, RHA1/RabF2a, ARA7/RabF2b, marker FM4-64. The expression SKD1E232Q, an ATPase-deficient...
Transport of cytoplasmically synthesized proteins into chloroplasts uses an import machinery present in the envelope membranes. To identify components this and to begin examine how these interact during transport, chemical cross-linking was performed on intact containing precursor trapped at a particular stage transport by ATP limitation. Large crosslinked complexes were observed using three different reversible homobifunctional cross-linkers. Three outer membrane (OEP86, OEP75, OEP34) one...
Abstract During reproduction in flowering plants, pollen grains form a tube that grows polarized fashion through the female tissues to eventually fertilize egg cell. These highly tubes have rapid rate of growth is supported by tip-focused delivery membrane and cell wall components. To gain better understanding how this regulated, we investigated function RABA4D, member Arabidopsis thaliana RabA4 subfamily Rab GTPase proteins. Here, show RABA4D was expressed pollen-specific manner enhanced...
Polyphosphoinositides represent a minor group of phospholipids, accounting for less than 1% the total. Despite their low abundance, these molecules have been implicated in various signalling and membrane trafficking events. Phosphatidylinositol 4-phosphate (PtdIns4P) is most abundant polyphosphoinositide. (32)Pi-labelling studies shown that turnover PtdIns4P rapid, but little known about where cell or plant this occurs. Here, we describe use lipid biosensor monitors dynamics living cells....
Polarized expansion of root hair cells in Arabidopsis thaliana is improperly controlled hair-defective rhd4-1 mutant plants, resulting hairs that are shorter and randomly form bulges along their length. Using time-lapse fluorescence microscopy hairs, we analyzed membrane dynamics after labeling with RabA4b, a marker for polarized trafficking hairs. This revealed stochastic loss recovery the RabA4b compartment tips growing consistent role RHD4 protein regulation these cells. The wild-type...
Abstract Keratins that are overexpressed selectively in human carcinomas may offer diagnostic and prognostic utility. In this study, we show high expression of keratin-17 (K17) predicts poor outcome patients with cervical cancer, at early or late stages disease, surpassing accuracy either tumor staging loss p27KIP1 as a negative marker setting. We investigated the mechanistic basis for biologic impact K17 through loss- gain-of-function experiments cervix, breast, pancreatic cancer cells....