A5025405144- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant Gene Expression Analysis
- Plant Molecular Biology Research
- Retinal and Optic Conditions
- Plant Reproductive Biology
- Retinal Diseases and Treatments
- Plant Water Relations and Carbon Dynamics
- Antioxidant Activity and Oxidative Stress
- Photoreceptor and optogenetics research
- Leaf Properties and Growth Measurement
- Ergonomics and Musculoskeletal Disorders
- Postharvest Quality and Shelf Life Management
- Plant responses to water stress
- Ocular Diseases and Behçet’s Syndrome
- Journalism and Media Studies
- Plant responses to elevated CO2
- Polysaccharides and Plant Cell Walls
- Plant and soil sciences
- Indoor Air Quality and Microbial Exposure
- Youth Culture and Social Dynamics
- Plant and Biological Electrophysiology Studies
- Microtubule and mitosis dynamics
- Ocular Oncology and Treatments
- Ocular Surface and Contact Lens
Instituto de Fisiología Vegetal
2007-2024
Consejo Nacional de Investigaciones Científicas y Técnicas
2013-2024
Universidad Nacional de La Plata
2004-2022
Universidad Católica de Córdoba
2015-2020
Vacuolar compartments associated with leaf senescence and the subcellular localization of senescence-specific cysteine-protease SAG12 (senescence-associated gene 12) were studied using specific fluorescent markers, expression reporter genes, analysis high-pressure frozen/freeze-substituted samples. Senescence-associated vacuoles (SAVs) intense proteolytic activity develop in peripheral cytoplasm mesophyll guard cells Arabidopsis soybean. The vacuolar identity these was confirmed by...
Abstract In yeast and mammals, the AAA ATPase Vps4p/SKD1 (for Vacuolar protein sorting 4/SUPPRESSOR OF K+ TRANSPORT GROWTH DEFECT1) is required for endosomal of secretory endocytic cargo. We identified a VPS4/SKD1 homolog in Arabidopsis thaliana, which localizes to cytoplasm multivesicular endosomes. addition, green fluorescent protein–SKD1 colocalizes on bodies with fusion Rab GTPases, such as ARA6/RabF1, RHA1/RabF2a, ARA7/RabF2b, marker FM4-64. The expression SKD1E232Q, an ATPase-deficient...
Massive degradation of photosynthetic proteins is the hallmark leaf senescence; however mechanism involved in chloroplast protein breakdown not completely understood. As small 'senescence-associated vacuoles' (SAVs) with intense proteolytic activity accumulate senescing leaves soybean and Arabidopsis, main goal this work was to determine whether SAVs are chloroplastic components. protease were readily detected through confocal microscopy naturally tobacco (Nicotiana tabacum L.). In detached...
Breakdown of leaf proteins, particularly chloroplast is a massive process in senescing leaves. In spite its importance internal N recycling, the mechanism(s) and enzymes involved are largely unknown. Senescence-associated vacuoles (SAVs) small, acidic with high cysteine peptidase activity. Chloroplast-targeted proteins re-localize to SAVs during senescence, suggesting that might be protein degradation. were undetectable mature, non-senescent tobacco Their abundance, visualized either...
Cellular proteins are extensively degraded during leaf senescence, and this correlates with an up-regulation of protease gene expression, particularly cysteine proteases. The objectives work were (i) to detect proteases associated senescence wheat leaves under different conditions (ii) find out their subcellular location. Activity labelling the biotinylated inhibitor DCG-04 detected five bands at 27, 36, 39, 42, 46 kDa in senescing continuous darkness. In-gel activity assays showed that...
.The apoplast, i.e., the cellular compartment external to plasma membrane, undergoes important changes during senescence. Apoplastic fluid volume increases quite significantly in senescing leaves, thereby diluting its contents. Its pH elevates by about 0.8 units, similar apoplast alkalization response abiotic stresses. The levels of 159 proteins decrease, whereas 24 increase relative abundance leaves. Around half apoplastic non-senescent leaves contain an N-terminal signal peptide for...
Senescence involves increased expression of proteases, which may participate in nitrogen recycling or cellular signalling. 2D zymograms detected two protein species with proteolytic activity senescing leaves Arabidopsis thaliana. A proteomic analysis revealed that both correspond to a subtilisin protease encoded by At3g14067, termed Senescence-Associated Subtilisin Protease (SASP). SASP mRNA levels and enzyme increase during leaf senescence the vegetative reproductive phase plant life cycle,...
Ear photosynthesis may be an important source of C for grain growth in water‐stressed plants cereals. The main objectives this work were to determine the stability photosynthetic apparatus and photochemical efficiency ears subjected post‐anthesis drought. Plants wheat ( Triticum aestivum L. cv. Granero INTA) grown pots under a rain shelter water stress (soil potential around −0.6 −0.8 MPa) starting 4 days after anthesis. Post‐anthesis drought substantially accelerated loss chlorophyll,...
The insect-trapping leaves of Dionaea muscipula provide a model for studying the secretory pathway an inducible plant system. leaf glands were induced with bovine serum albumin to secrete proteases that characterized via zymogram activity gels over 6-day period. accompanying morphological changes endoplasmic reticulum (ER) and Golgi analyzed using 3D electron tomography preserved by high-pressure freezing/freeze substitution methods. Secretion multiple cysteine aspartic occurred...
Purpose: To present a case of complicated posterior melanocytoma that was successfully treated with intravitreal bevacizumab. Case report: A 50-year-old Caucasian man referred sudden-onset metamorphopsia and decreased vision in his right eye over the course last 2 months. His best-corrected visual acuity 20/80 poorer than Jaeger 14 eye, 20/20 1 left eye. In fundus, there melanocytic lesion occupying inferotemporal quadrant optic disk, extending to adjacent choroid inferiorly; nerve edema,...