A5046429658- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Agronomic Practices and Intercropping Systems
- Nematode management and characterization studies
- Plant-Microbe Interactions and Immunity
- Plant tissue culture and regeneration
- Microbial Metabolites in Food Biotechnology
- Photosynthetic Processes and Mechanisms
- Plant Disease Resistance and Genetics
- Phytase and its Applications
- Soybean genetics and cultivation
- Transgenic Plants and Applications
- Plant Micronutrient Interactions and Effects
- Mycorrhizal Fungi and Plant Interactions
- Wastewater Treatment and Nitrogen Removal
- Plant responses to water stress
- Biofuel production and bioconversion
- Algal biology and biofuel production
- Plant Pathogenic Bacteria Studies
- Polyamine Metabolism and Applications
- Plant Virus Research Studies
- Coastal wetland ecosystem dynamics
- Polymer-Based Agricultural Enhancements
- Polysaccharides and Plant Cell Walls
- Food composition and properties
Centre National de la Recherche Scientifique
2009-2024
Laboratoire des Interactions Plantes Micro-Organismes
2013-2024
Université Toulouse III - Paul Sabatier
1999-2024
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021-2024
Université de Toulouse
2016-2024
Interactions Arbres-Microorganismes
1999-2014
Institut National de la Recherche Agronomique
2010-2013
Rothamsted Research
1983-2013
Laboratoire de Biogenèse Membranaire
2007
Instituto de Biologia Molecular e Celular
2003
Recognition of microbial patterns by host pattern recognition receptors is a key step in immune activation multicellular eukaryotes. Peptidoglycans (PGNs) are major components bacterial cell walls that possess immunity-stimulating activities metazoans and plants. Here we show PGN sensing immunity to infection Arabidopsis thaliana requires three lysin-motif (LysM) domain proteins. LYM1 LYM3 plasma membrane proteins physically interact with PGNs mediate sensitivity structurally different from...
Remorin proteins have been hypothesized to play important roles during cellular signal transduction processes. Induction of some members this multigene family has reported biotic interactions. However, no host-bacteria interactions assigned remorin until now. We used root nodule symbiosis between Medicago truncatula and Sinorhizobium meliloti study the a that is specifically induced nodulation. Here we show oligomeric protein attaches host plasma membrane surrounding bacteria controls...
Abstract Several studies have provided new insights into the role of sphingolipid/sterol-rich domains so-called lipid rafts plasma membrane (PM) from mammalian cells, and more recently leaves, cell cultures, seedlings higher plants. Here we show that raft domains, defined as Triton X-100-insoluble membranes, can also be prepared Medicago truncatula root PMs. These been extensively characterized by ultrastructural well analysis their content in lipids proteins. M. are shown to enriched...
Rhizosphere interactions are affected by many different regulatory signals. As yet, however, only a few have been identified. Signals, definition, contain information, react with receptor, and elicit response. Signals may thus represent the highest level of evolved response in rhizosphere communities and, that sense, occupy supreme control point. At same time, some signals function as modulators downstream responses, rather than on/off switches. To assess these possibilities, several between...
Abstract LYK3 is a lysin motif receptor-like kinase of Medicago truncatula, which essential for the establishment nitrogen-fixing, root nodule symbiosis with Sinorhizobium meliloti. putative receptor S. meliloti Nod factor signals, but little known how it regulated and transduces these symbiotic signals. In screen LYK3-interacting proteins, we identified M. truncatula Plant U-box protein 1 (PUB1) as an interactor domain. planta, both proteins are localized interact in plasma membrane. PUB1...
Summary Plant L ys M proteins control the perception of microbial‐derived N ‐acetylglucosamine compounds for establishment symbiosis or activation plant immunity. This raises questions about how plants, and notably legumes, can differentiate friends foes using similar molecular actors whether any receptors intervene in both resistance. To study this question, nfp lyk3 ‐receptor like kinase mutants edicago truncatula that are affected early steps nodulation, were analysed following...
Medicago truncatula contains a family of at least five genes related to AUX1 Arabidopsis thaliana (termed MtLAX for truncatula-like genes). The high sequence similarity between the encoded proteins and implies that encode auxin import carriers. are expressed in roots other organs, suggesting they play pleiotropic roles uptake. In primary roots, preferentially root tips, particularly provascular bundles caps. During lateral nodule development, primordia, cells were probably derived from...
The gln-gamma gene, which specifies the gamma subunit of glutamine synthetase in Phaseolus vulgaris L., has been isolated and regulatory properties its promoter region analyzed transgenic Lotus corniculatus plants. A 2-kilobase fragment from 5'-flanking conferred a strongly nodule-enhanced pattern expression on beta-glucuronidase reporter gene. Parallel studies another gene (gln-beta) showed that 1.7-kilobase directed 20-fold to 140-fold higher levels roots than shoots. Histochemical...
Receptor(-like) kinases with Lysin Motif (LysM) domains in their extracellular region play crucial roles during plant interactions microorganisms; e.g. Arabidopsis thaliana CERK1 activates innate immunity upon perception of fungal chitin/chitooligosaccharides, whereas Medicago truncatula NFP and LYK3 mediate signalling bacterial lipo-chitooligosaccharides, termed Nod factors, the establishment mutualism nitrogen-fixing rhizobia. However, little is still known about exact activation...
While chitooligosaccharides (COs) derived from fungal chitin are potent elicitors of defense reactions, structurally related signals produced by certain bacteria and fungi, called lipo-chitooligosaccharides (LCOs), play important roles in the establishment symbioses with plants. Understanding how plants distinguish between friend foe through perception these is a major challenge. We report synthesis range COs LCOs, including photoactivatable probes, to characterize membrane protein legume...
The establishment of the symbiosis between legume plants and rhizobial bacteria depends on production lipo-chitooligosaccharidic signals (the Nod factors) that are specifically recognized by roots host plant. In Medicago truncatula, specific recognition Sinorhizobium meliloti its factors requires NFP (Nod factor perception) gene, which encodes a putative serine/threonine receptor-like kinase (RLK). extracellular region this protein contains three tandem lysin motifs (LysMs), short peptide...
Summary This paper describes the characteristics of a binding site for major, lipo‐oligosaccharide Nod factor Rhizobium meliloti in roots symbiotic host plant, Medicago truncatula . Chemically synthesized NodRm‐IV(Ac, S, C16:2) was labelled by tritiation to specific activity 56 Ci mmol −1 and this ligand shown be biologically active root hair deformation assay at 10 −11 M. Binding particulate fraction from found saturable reversible with an affinity ( K d ) 86 nM were consistent single class...
PUB1, an E3 ubiquitin ligase, which interacts with and is phosphorylated by the LYK3 symbiotic receptor kinase, negatively regulates rhizobial infection nodulation during nitrogen-fixing root nodule symbiosis in Medicago truncatula. In this study, we show that PUB1 also DOES NOT MAKE INFECTIONS 2, key kinase of common signaling pathway, required for both arbuscular mycorrhizal (AM) endosymbioses. We here expression activated successive stages colonization Rhizophagus irregularis compatible...
The specific activity of plant NADH-dependent glutamate synthase (NADH-GOGAT) in root nodules Phaseolus vulgaris L. is over threefold higher than the ferredoxin-dependent GOGAT. NADH-GOGAT composed two distinct isoenzymes (NADH-GOGAT I and II) which can be separated from crude nodule extracts by ion-exchange chromatography. Both have been purified to apparent homogeneity shown monomeric proteins with similar M(r)s about 200,000. They are both for NADH as reductant. An investigation their...
Abstract To study the role of LecRK (lectin-like receptor kinase) genes in legumerhizobia symbiosis, we have characterized four Medicago truncatula Gaernt. that are most highly expressed roots. Three these genes, MtLecRK7;1, MtLecRK7;2, and MtLecRK7;3, encode proteins closely related to Class A LecRKs Arabidopsis, whereas protein encoded by fourth gene, MtLecRK1;1, is similar a B Arabidopsis LecRK. All show strongly enhanced root expression, detailed studies on MtLecRK1;1 MtLecRK7;2 revealed...