A5073333495- Lipid metabolism and biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Photosynthetic Processes and Mechanisms
- Fatty Acid Research and Health
- Enzyme Catalysis and Immobilization
- Rock Mechanics and Modeling
- Aquaculture Nutrition and Growth
- Plant biochemistry and biosynthesis
- Algal biology and biofuel production
- Geotechnical and Geomechanical Engineering
- Plant tissue culture and regeneration
- Animal Genetics and Reproduction
- Plant Molecular Biology Research
- Sphingolipid Metabolism and Signaling
- Transgenic Plants and Applications
- Plant Reproductive Biology
- Wheat and Barley Genetics and Pathology
- Lipid Membrane Structure and Behavior
- Soybean genetics and cultivation
- Insect-Plant Interactions and Control
- Plant Parasitism and Resistance
- Biodiesel Production and Applications
- Genetically Modified Organisms Research
- Numerical methods in engineering
- Phytase and its Applications
Rothamsted Research
2016-2025
Newham University Hospital
2023
University of Pretoria
2011-2023
Ghent University
2023
VIB-UGent Center for Plant Systems Biology
2023
University of Kentucky
2021
University of Minnesota
2016
Twin Cities Orthopedics
2016
Google (United States)
2014
Canadian Society of Intestinal Research
2011
Coccolithophores have influenced the global climate for over 200 million years. These marine phytoplankton can account 20 per cent of total carbon fixation in some systems. They form blooms that occupy hundreds thousands square kilometres and are distinguished by their elegantly sculpted calcium carbonate exoskeletons (coccoliths), rendering them visible from space. Although coccolithophores export organic matter calcite to sea floor, they also release CO2 calcification process. Hence, a...
The trafficking and function of cell surface proteins in eukaryotic cells may require association with detergent-resistant sphingolipid- sterol-rich membrane domains. aim this work was to obtain evidence for lipid domain phenomena plant membranes. A protocol prepare Triton X-100 membranes (DRMs) developed using Arabidopsis (Arabidopsis thaliana) callus comparative proteomics approach two-dimensional difference gel electrophoresis liquid chromatography-tandem mass spectrometry revealed that...
Abstract Land plant aerial organs are covered by a hydrophobic layer called the cuticle that serves as waterproof barrier protecting plants against desiccation, ultraviolet radiation, and pathogens. Cuticle consists of cutin matrix well cuticular waxes in which very-long-chain (VLC) alkanes major components, representing up to 70% total wax content Arabidopsis (Arabidopsis thaliana) leaves. However, despite its involvement formation, alkane-forming pathway is still largely unknown. To...
cis -Jasmone, or ( Z )-jasmone, is well known as a component of plant volatiles, and its release can be induced by damage, for example during insect herbivory. Using the olfactory system lettuce aphid to investigate volatiles from plants avoided this insect, )-jasmone was found electrophysiologically active also repellent in laboratory choice tests. In field studies, repellency traps demonstrated damson-hop aphid, with cereal aphids numbers were reduced plots winter wheat treated )-jasmone....
In land plants, very-long-chain (VLC) alkanes are major components of cuticular waxes that cover aerial organs, mainly acting as a waterproof barrier to prevent nonstomatal water loss. Although thoroughly investigated, plant alkane synthesis remains largely undiscovered. The Arabidopsis thaliana ECERIFERUM1 (CER1) protein has been recognized an essential element wax synthesis; nevertheless, its function elusive. this study, screen for CER1 physical interaction partners was performed....
Summary Omega‐3 (also called n ‐3) long‐chain polyunsaturated fatty acids (≥C20; LC‐PUFAs) are of considerable interest, based on clear evidence dietary health benefits and the concurrent decline global sources (fish oils). Generating alternative transgenic plant omega‐3 LC‐PUFAs, i.e. eicosapentaenoic acid (20:5 ‐3, EPA) docosahexaenoic (22:6 DHA) has previously proved problematic. Here we describe a set heterologous genes capable efficiently directing synthesis these in seed oil crop...
We have engineered the diatom Phaeodactylum tricornutum to accumulate high value omega-3 long chain polyunsaturated fatty acid docosahexaenoic (DHA). This was achieved by generation of transgenic strains in which Δ5-elongase from picoalga Ostreococcus tauri expressed augment endogenous biosynthetic pathway. Expression heterologous elongase resulted an eight-fold increase content, representing a marked and valuable change profile this microalga. Importantly, DHA shown triacylglycerols, with...
Abstract ω6- and ω3-polyunsaturated C20 fatty acids represent important components of the human diet. A more regular consumption an accordingly sustainable source these compounds are highly desirable. In contrast with very high levels to which industrial have be enriched in plant oils for competitive use as chemical feedstocks, much lower percentages very-long-chain polyunsaturated (VLCPUFA) edible would satisfy nutritional requirements. Seed-specific expression transgenic tobacco (Nicotiana...
γ-Linolenic acid (GLA; C18:3 Δ 6,9,12 ) is a component of the seed oils evening primrose ( Oenothera spp.), borage Borago officinalis L.), and some other plants. It widely used as dietary supplement for treatment various medical conditions. GLA synthesized by 6 -fatty desaturase using linoleic (C18:2 9,12 substrate. To enable production in conventional oilseeds, we have isolated cDNA encoding from developing seeds confirmed its function expression transgenic tobacco Analysis leaf lipids...
Summary The ‘substantial equivalence’ of three transgenic wheats expressing additional high‐molecular‐weight subunit genes and the corresponding parental lines (two plus a null transformant) was examined using metabolite profiling samples grown in replicate field trials on two UK sites (Rothamsted, Hertfordshire Long Ashton, near Bristol) for 3 years. Multivariate comparison proton nuclear magnetic resonance spectra polar metabolites extracted with deuterated methanol–water showed stronger...
Abstract Several studies have provided new insights into the role of sphingolipid/sterol-rich domains so-called lipid rafts plasma membrane (PM) from mammalian cells, and more recently leaves, cell cultures, seedlings higher plants. Here we show that raft domains, defined as Triton X-100-insoluble membranes, can also be prepared Medicago truncatula root PMs. These been extensively characterized by ultrastructural well analysis their content in lipids proteins. M. are shown to enriched...
Very-long-chain fatty acids (VLCFAs) are synthesized as acyl-CoAs by the endoplasmic reticulum-localized elongase multiprotein complex. Two Arabidopsis genes putative homologues of recently identified yeast 3-hydroxy-acyl-CoA dehydratase ( PHS1 ), third enzyme We showed that PASTICCINO2 PAS2 ) was able to restore phs1 cytokinesis defects and sphingolipid long chain base overaccumulation. Conversely, expression complement developmental accumulation bases pas2–1 mutant. The mutant...
In plants, very-long-chain fatty acids (VLCFAs; >18 carbon) are precursors of sphingolipids, triacylglycerols, cuticular waxes, and suberin. VLCFAs synthesized by a multiprotein membrane-bound acid elongation system that catalyzes four successive enzymatic reactions: condensation, reduction, dehydration, second reduction. A bioinformatics survey the Arabidopsis (Arabidopsis thaliana) genome has revealed two sequences homologous to YBR159w encoding Saccharomyces cerevisiae beta-ketoacyl...
Abstract Very-long-chain fatty acids (VLCFAs) are essential for many aspects of plant development and necessary the synthesis seed storage triacylglycerols, epicuticular waxes, sphingolipids. Identification acetyl-CoA carboxylase PASTICCINO3 3-hydroxy acyl-CoA dehydratase PASTICCINO2 revealed that VLCFAs important cell proliferation tissue patterning. Here, we show immunophilin PASTICCINO1 (PAS1) is also required VLCFA synthesis. Impairment PAS1 function results in reduction levels...
It is of adaptive value for a plant to prepare its defenses when threat detected, and certain volatiles associated with insect damage, such as cis -jasmone (CJ), are known switch-on defense metabolism. We used aphid parasitoid responses Arabidopsis thaliana model system studying gene expression chemistry impact at different trophic levels. Differential induced occurred specialist generalist insects: the aphid, Myzus persicae , was repelled, whereas specialist, Lipaphis erysimi attracted;...