A5039759499- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Lipid metabolism and biosynthesis
- Redox biology and oxidative stress
- Plant nutrient uptake and metabolism
- ATP Synthase and ATPases Research
- Plant responses to water stress
- Plant Stress Responses and Tolerance
- Service-Oriented Architecture and Web Services
- Hemoglobin structure and function
- Glutathione Transferases and Polymorphisms
- Autonomous Vehicle Technology and Safety
- Heme Oxygenase-1 and Carbon Monoxide
- Business Process Modeling and Analysis
- Adipose Tissue and Metabolism
- Traffic Prediction and Management Techniques
- Genomics, phytochemicals, and oxidative stress
- Endoplasmic Reticulum Stress and Disease
- Mass Spectrometry Techniques and Applications
- Technical Engine Diagnostics and Monitoring
- Plant biochemistry and biosynthesis
- Flexible and Reconfigurable Manufacturing Systems
- ERP Systems Implementation and Impact
- Engine and Fuel Emissions
- Traffic and Road Safety
University of Bonn
2015-2023
Institute of Crop Science
2023
University of Münster
2017-2023
Institute of Plant Biology and Biotechnology
2023
Leonardo (United Kingdom)
2009
European Research Center for Information Systems
2009
Centre de Robotique
2005
École Nationale Supérieure des Mines de Paris
2005
Hôpital Saint-Michel
2005
Growth and development of plants is ultimately driven by light energy captured through photosynthesis. ATP acts as universal cellular cofactor fuelling all life processes, including gene expression, metabolism, transport. Despite a mechanistic understanding biochemistry, dynamics in the living plant have been largely elusive. Here, we establish MgATP2- measurement using fluorescent protein biosensor ATeam1.03-nD/nA. We generate Arabidopsis sensor lines investigate vitro under conditions...
Summary Mitochondria host vital cellular functions, including oxidative phosphorylation and co‐factor biosynthesis, which are reflected in their proteome. At the level plant mitochondria organized into hundreds of discrete functional entities, undergo dynamic fission fusion. It is individual organelle that operates living cell, yet biochemical physiological assessments have exclusively focused on characteristics large populations mitochondria. Here, we explore protein composition an average...
Summary Hydrogen peroxide (H 2 O ) is ubiquitous in cells and at the centre of developmental programmes environmental responses. Its chemistry makes H notoriously hard to detect dynamically, specifically high resolution. Genetically encoded sensors overcome persistent shortcomings, but pH sensitivity, silencing expression a limited concept sensor behaviour vivo have hampered any meaningful sensing living plants. We established monitoring cytosol mitochondria Arabidopsis with fusion protein...
Seeds preserve a far developed plant embryo in quiescent state. Seed metabolism relies on stored resources and is reactivated to drive germination when the external conditions are favorable. Since switchover from quiescence reactivation provides remarkable case of cell physiological transition we investigated earliest events energy redox Arabidopsis seeds at imbibition. By developing fluorescent protein biosensing intact seeds, observed ATP accumulation oxygen uptake within minutes,...
Plant organelle function must constantly adjust to environmental conditions, which requires dynamic coordination. Ca(2+) signaling may play a central role in this process. Free dynamics are tightly regulated and differ markedly between the cytosol, plastid stroma, mitochondrial matrix. The mechanistic basis of compartment-specific is poorly understood. Here, we studied At-MICU, an EF-hand protein Arabidopsis thaliana with homology constituents uniporter machinery mammals. MICU binds...
Summary Hypoxia regularly occurs during plant development and can be induced by the environment through, for example, flooding. To understand how tissue physiology responds to progressing oxygen restriction, we aimed monitor subcellular in real time vivo . We establish a fluorescent protein sensor‐based system multiparametric monitoring of dynamic changes living Arabidopsis thaliana leaves exemplify its applicability hypoxia stress. By cytosolic dynamics magnesium adenosine 5'‐triphosphate,...
Abstract Metabolic fluctuations in chloroplasts and mitochondria can trigger retrograde signals to modify nuclear gene expression. Mobile likely be involved are reactive oxygen species (ROS), which operate protein redox switches by oxidation of specific cysteine residues. Redox buffers, such as the highly reduced glutathione pool, serve reservoirs reducing power for several ROS-scavenging ROS-induced damage repair pathways. Formation disulfide a shift potential (EGSH) toward less negative...
Significance By studying in vivo changes of ATP levels the plastids and cytosol Arabidopsis thaliana using a FRET-based sensor, we show that plastidic concentrations cotyledon, hypocotyl, root 10-day-old seedlings are significantly lower than cytosolic concentrations. We chloroplasts consume rapidly import into mature is impeded by low density NTT transporter. Hence, unlike diatoms, where imported to support Calvin–Benson–Bassham (CBB) cycle, do not balance ATP:NADPH ratio importing from...
Over the recent years, several proteins that make up mitochondrial calcium uniporter complex (MCUC) mediating Ca2+uptake into matrix have been identified in mammals, including channel-forming protein MCU. Although six MCU gene homologs are conserved model plant Arabidopsis (Arabidopsis thaliana) which mitochondria can accumulate Ca2+, a functional characterization of has lacking. Using electrophysiology, we show one isoform, AtMCU1, gives rise to Ca2+-permeable channel activity be observed...
NADH and NAD+ are a ubiquitous cellular redox couple. Although the central role of NAD in plant metabolism its regulatory have been investigated extensively at biochemical level, analyzing subcellular dynamics living tissues has challenging. Here, we established live monitoring NADH/NAD+ plants using genetically encoded fluorescent biosensor Peredox-mCherry. We Peredox-mCherry lines Arabidopsis (Arabidopsis thaliana) validated biophysical properties sensor that critical for planta...
Malate and citrate underpin the characteristic flexibility of central plant metabolism by linking mitochondrial respiratory with cytosolic biosynthetic pathways. However, identity carrier proteins that influence both processes has remained elusive. Here we show a systems approach DICARBOXYLATE CARRIER 2 (DIC2) facilitates malate-citrate exchange in vivo Arabidopsis thaliana. DIC2 knockout (dic2-1) retards growth vegetative tissues. In vitro organello analyses demonstrate preferentially...
Abstract Redox processes are at the heart of universal life processes, such as metabolism, signaling, or folding secreted proteins. landscapes differ between cell compartments and strictly controlled to tolerate changing conditions avoid dysfunction. While a sophisticated antioxidant network counteracts oxidative stress, our understanding reductive stress responses remains fragmentary. Here, we observed root growth impairment in Arabidopsis thaliana mutants mitochondrial alternative oxidase...
Glycolysis generates methylglyoxal (MGO) as an unavoidable, cytotoxic by-product in plant cells. MGO scavenging is performed by the glyoxalase system, which produces d-lactate end product. d-Lactate dehydrogenase (d-LDH) encoded a single gene Arabidopsis (Arabidopsis thaliana; At5g06580). It catalyzes vitro oxidation of to pyruvate using flavin adenine dinucleotide cofactor; knowledge its function context cell remains sketchy. Blue native-polyacrylamide gel electrophoresis mitochondrial...
Plant glutathione S-transferases (GSTs) are glutathione-dependent enzymes with versatile functions, mainly related to detoxification of electrophilic xenobiotics and peroxides. The Arabidopsis (Arabidopsis thaliana) genome codes for 53 GSTs, divided into seven subclasses; however, understanding their precise functions is limited. A recent study showed that class II TGA transcription factors TGA2, TGA5, TGA6 essential tolerance UV-B-induced oxidative stress this associated an antioxidative...
Abstract Oxidative protein folding in the endoplasmic reticulum (ER) depends on coordinated action of disulfide isomerases and ER oxidoreductins (EROs). Strict dependence ERO activity molecular oxygen as final electron acceptor implies that oxidative other processes are severely compromised under hypoxia. Here, we isolated viable Arabidopsis thaliana ero1 ero2 double mutants highly sensitive to reductive stress To elucidate specific redox dynamics vivo, expressed glutathione potential (EGSH)...
Ca2+ signaling is central to plant development and acclimation. While Ca2+-responsive proteins have been investigated intensely in plants, only a few Ca2+-permeable channels identified, our understanding of how intracellular fluxes facilitated remains limited. Arabidopsis thaliana homologs the mammalian channel-forming mitochondrial calcium uniporter (MCU) protein showed transport activity vitro. Yet, evolutionary complexity MCU proteins, as well reports about alternative systems unperturbed...
Abstract A characteristic feature of most plants is their ability to perform photosynthesis, which ultimately provides energy and organic substrates life. Photosynthesis dominates chloroplast physiology but represents only a fraction the tightly interconnected metabolic network that spans entire cell. Here, we explore how photosynthetic activity affects physiological status in cell compartments beyond chloroplast. We develop precision live monitoring subcellular under illumination...
ABSTRACT Metabolic fluctuations in chloroplasts and mitochondria can trigger retrograde signals to modify nuclear gene expression. Mobile likely be involved are reactive oxygen species (ROS), which operate protein redox switches by oxidation of specific cysteine residues. Redox buffers such as the highly reduced glutathione pool serve reservoirs reducing power for several ROS scavenging ROS-induced damage repair pathways. Formation disulfide (GSSG) a shift potential ( E GSH ) towards less...
Abstract Growth and development of plants is ultimately driven by light energy captured through photosynthesis. ATP acts as universal cellular cofactor fuelling all life processes, including gene expression, metabolism, transport. Despite a mechanistic understanding biochemistry, dynamics in the living plant have been largely elusive. Here we establish live MgATP 2− assessment using fluorescent protein biosensor ATeam1.03-nD/nA. We generate Arabidopsis sensor lines investigate vitro under...
ABSTRACT Plant glutathione S -transferases (GSTs) are glutathione-dependent enzymes with versatile functions, mainly related to detoxification of electrophilic xenobiotics and peroxides. The Arabidopsis genome codes for 53 GSTs, divided into seven subclasses, however understanding their precise functions is limited. A recent study showed that class II TGA transcription factors TGA2, TGA5 TGA6 essential tolerance UV-B-induced oxidative stress this associated an antioxidative function...
Setting up a digital driver behavior model represents one major approach to design and validate active safety devices during their development phase. The present contribution introduces our predictive of actions on car controls (steering wheel pedals). Its core is based the combined use neural networks genetic algorithms. This was set using behavioral database gathered from test track experiment. paper details different stages followed construct model. It shows validation results another...