A5040470049- Metalloenzymes and iron-sulfur proteins
- Redox biology and oxidative stress
- Photosynthetic Processes and Mechanisms
- Plant Virus Research Studies
- Plant Micronutrient Interactions and Effects
- Heat shock proteins research
- Plant Stress Responses and Tolerance
- Enzyme Structure and Function
- Glutathione Transferases and Polymorphisms
- Legume Nitrogen Fixing Symbiosis
- Metal-Catalyzed Oxygenation Mechanisms
- RNA modifications and cancer
- Endoplasmic Reticulum Stress and Disease
- Insect and Pesticide Research
- Plant Reproductive Biology
- GABA and Rice Research
- Insect-Plant Interactions and Control
- Plant Molecular Biology Research
- Nematode management and characterization studies
- Soybean genetics and cultivation
- Toxoplasma gondii Research Studies
- Iron oxide chemistry and applications
- Plant-Microbe Interactions and Immunity
- Electron Spin Resonance Studies
- Mitochondrial Function and Pathology
Centre National de la Recherche Scientifique
2007-2025
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2025
Institut Agro Montpellier
2019-2025
Université de Montpellier
2012-2025
Institut des Sciences des Plantes de Montpellier
2012-2024
Agropolis International
2009-2022
Institut de Recherche pour le Développement
2007-2022
Laboratoire des Interactions Plantes Micro-Organismes
2018-2022
Brazilian Agricultural Research Corporation
2020
Centre de Coopération Internationale en Recherche Agronomique pour le Développement
2012-2019
Iron (Fe) is an essential micronutrient for plant growth and development. Any defects in the maintenance of Fe homeostasis will alter productivity quality their derived products. In Arabidopsis (Arabidopsis thaliana), transcription factor ILR3 plays a central role controlling homeostasis. this study, we identified bHLH121 as ILR3-interacting factor. Interaction studies showed that also interacts with three closest homologs (i.e., basic-helix-loop-helix 34 [bHLH34], bHLH104, bHLH115). bhlh121...
The brown midrib mutations are among the earliest described in maize. Plants containing a mutation exhibit reddish pigmentation of leaf starting when there four to six leaves. These known alter lignin composition and digestibility plants therefore constitute prime candidates breeding silage Here, we show that two independent midrib3 (bm3) have resulted from structural changes COMT gene, which encodes enzyme O-methyltransferase (COMT; EC 2.1.1.6), involved biosynthesis. Our results indicate...
Disruption of the two thioredoxin genes in yeast dramatically affects cell viability and growth. Expression of<i>Arabidopsis</i> AtTRX3 the<i>Saccharomyces</i> Δ strain EMY63 restores a wild-type cycle, ability to grow on methionine sulfoxide, H<sub>2</sub>O<sub>2</sub> tolerance. In order isolate targets related these phenotypes, we prepared C35S (<i>Escherichia coli</i> numbering) mutant stabilize intermediate disulfide bridged complex added polyhistidine N-terminal extension purify...
Summary Iron (Fe) homeostasis is crucial for all living organisms. In mammals, an integrated posttranscriptional mechanism couples the regulation of both Fe deficiency and excess responses. Whether in plants control involving common players regulates responses to still be determined. this study, molecular, genetic biochemical approaches were used investigate transcriptional excess. A activator shortage Arabidopsis, called bHLH105/ILR3, was found also negatively regulate expression ferritin...
We studied the temporal and spatial pattern of lipid transfer protein (LTP) gene expression, as well localization this protein, in maize. Using an LTP gene, we observed accumulation mRNA embryos endosperms during seed maturation. expression was also investigated young seedlings. After germination, level coleoptile increased, with a maximum at 7 days, whereas levels were low scutellum negligible roots. The high found coleoptiles confirmed by situ hybridization. Moreover, appeared to be...
We investigated heat shock (HS)-triggered Ca(2+) signalling transduced by a sensor, calmodulin (CaM), linked to early transcriptome changes of HS-responsive genes in rice. observed biphasic [Ca(2+) ](cyt) signature root cells that was distinct from epicotyl and leaf cells, which showed monophasic response after HS. Treatment with A23187 generated an intense sustained increase Conversely, treatment chelator, L-type channel blocker CaM antagonist, but not intracellular release inhibitor,...
Nfu-type proteins are essential in the biogenesis of iron–sulfur (Fe-S) clusters numerous organisms. A number phenotypes including low levels Fe-S cluster incorporation associated with deletion gene encoding a chloroplast-specific protein, Nfu2 from Arabidopsis thaliana (AtNfu2). Here, we report that recombinant AtNfu2 is able to assemble both [2Fe-2S] and [4Fe-4S] clusters. Analytical data gel filtration studies support cluster/protein stoichiometries one cluster/homotetramer...
Plastid-localized NADPH-dependent thioredoxin reductase C (NTRC) is a unique NTR enzyme containing both and domains in single polypeptide. Arabidopsis thaliana NTRC knockout lines (ntrc) show retarded growth, especially under short-day (SD) photoperiods. This study identified chloroplast processes that accounted for growth reduction SD-acclimated ntrc. The strongest ntrc occurred photoperiods with nights longer than 14 h, whereas of the gene did not alter circadian-clock-controlled...
Ribonuclease III (RNaseIII) is responsible for processing and maturation of RNA precursors into functional rRNA, mRNA other small RNA. In contrast to bacterial yeast cells, higher eukaryotes contain at least three classes RNaseIII, including class IV or dicer-like proteins. Here, we describe the characterization AtRTL2, an Arabidopsis thaliana RNaseIII-like protein that belongs a family genes distinct from dicer family. We demonstrate AtRTL2 required 3′external transcribed spacer (ETS)...
The adaptation of Rice yellow mottle virus (RYMV) to recessive resistance mediated by the rymv1-2 allele has been reported as a model study emergence and evolution virulent variants. virulence factors have identified eukaryotic translation initiation factor eIF(iso)4G1 viral genome-linked protein (VPg), respectively, but molecular mechanisms involved in their interaction are still unknown. In this study, we demonstrated direct between RYMV VPg central domain rice both vitro, using...
Plant malate dehydrogenase (MDH) isoforms are found in different cell compartments and function key metabolic pathways. It is well known that the chloroplastic NADP-dependent MDH activities strictly redox regulated controlled by light. However, dependence of other NAD-dependent have been less studied. Here, we show vitro biochemical characterization major cytosolic isoform (cytMDH1) sensitive to H2O2 through sulfur oxidation cysteines methionines. CytMDH1 affects kinetics, secondary...
Several key cellular functions depend on proteins harboring an iron–sulfur (Fe-S) cofactor. As these Fe-S localize to several subcellular compartments, they require a dedicated machinery for cofactor assembly. For instance, in plants and algae there are cluster synthesis pathways localizing the cytosol, but also present mitochondrion chloroplast, 2 organelles of endosymbiotic origin. Toxoplasma gondii is plastid-bearing parasitic protist responsible pathology affecting humans other...
All organisms contain thioredoxin (TRX), a regulatory thiol:disulfide protein that reduces disulfide bonds in target proteins. Unlike animals and yeast, plants numerous TRXs for which no function has been assigned vivo . Recent vitro proteomic approaches have opened the way to identification of >100 TRX putative targets, but none plant can be specifically associated. In contrast, methodologies, including classical yeast two-hybrid (Y2H) systems, failed reveal expected high number targets....
In plants RNA silencing is a host defense mechanism against viral infection, in which double-strand processed into 21-24-nt short interfering (siRNA). Silencing spreads from cell to and systemically through sequence-specific signal limit the propagation of virus. To counteract this mechanism, viruses encode suppressors silencing. The P1 protein encoded by rice yellow mottle virus (RYMV) displays suppression activity with variable efficiency, according isolates that they originated from....
The rymv1-2 and rymv1-3 alleles of the RYMV1 resistance to Rice yellow mottle virus (RYMV), coded by an eIF(iso)4G1 gene, occur in a few cultivars Asiatic (Oryza sativa) African (O. glaberrima) rice species, respectively. most salient feature breaking (RB) process is converse genetic barrier breakdown. This specificity modulated amino acid (glutamic vs. threonine) at codon 49 Viral Protein genome-linked (VPg), position which adjacent virulence codons 48 52. Isolates with glutamic (E) do not...
Summary In monocotyledons, the root system is mostly composed of postembryonic shoot‐borne roots called crown roots. rice ( Oryza sativa ), auxin promotes initiation via LOB ‐domain transcription factor LBD ) CROWN ROOTLESS 1 CRL 1); however, gene regulatory network downstream remains largely unknown. We tested transcriptional activity in yeast and planta , identified 1‐regulated genes using an inducible expression a transcriptome analysis, used situ hybridization to demonstrate coexpression...
The brown midrib mutations are among the earliest described in maize.Plants containing a mutation exhibit reddish pigmentation of leaf starting when there four to six leaves.These known alter lignin composition and digestibility plants therefore constitute prime candidates breeding silage maize.Here, we show that two independent midrib3 (bm3) have resulted from structural changes COMT gene, which encodes enzyme O-methyltransferase (COMT; EC 2.1.1.6),involved biosynthesis.Our results indicate...