A5019033598- Plant Micronutrient Interactions and Effects
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Plant responses to water stress
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Aluminum toxicity and tolerance in plants and animals
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Cellular transport and secretion
- Plant Genetic and Mutation Studies
- Phytase and its Applications
- Porphyrin Metabolism and Disorders
- Agriculture and Rural Development Research
- Animal Behavior and Reproduction
- Calcium signaling and nucleotide metabolism
- Biochemical Acid Research Studies
- Archaeology and ancient environmental studies
- Plant tissue culture and regeneration
- Heavy metals in environment
- Lysosomal Storage Disorders Research
- Metalloenzymes and iron-sulfur proteins
- Plant Parasitism and Resistance
- Seed Germination and Physiology
- Rice Cultivation and Yield Improvement
Pontificia Universidad Católica de Chile
2005-2024
Millennium Engineering and Integration (United States)
2018
University of Wisconsin–Madison
2012-2015
Institut des Sciences des Plantes de Montpellier
2010-2015
Institut Agro Montpellier
2011-2013
Université de Montpellier
2011-2013
Institut National de la Recherche Agronomique
2009-2011
Centre National de la Recherche Scientifique
2009
Universidad de Santiago de Chile
2002
University of Notre Dame
2002
Iron (Fe) is an essential micronutrient for plant growth and development. Any defects in the maintenance of Fe homeostasis will alter productivity quality their derived products. In Arabidopsis (Arabidopsis thaliana), transcription factor ILR3 plays a central role controlling homeostasis. this study, we identified bHLH121 as ILR3-interacting factor. Interaction studies showed that also interacts with three closest homologs (i.e., basic-helix-loop-helix 34 [bHLH34], bHLH104, bHLH115). bhlh121...
Deciphering how cellular iron (Fe) pools are formed, where they localized, and which ones remobilized represents an important challenge to better understand Fe homeostasis. The recent development of imaging techniques, adapted plants, has helped gain insight into these events. We have analyzed the localization during embryo in Arabidopsis (Arabidopsis thaliana) with improved histochemical staining based on Perls coloration intensified by a second reaction diaminobenzidine hydrogen peroxide....
We present data supporting a general role for FERRIC REDICTASE DEFECTIVE3 (FRD3), an efflux transporter of the efficient iron chelator citrate, in maintaining homeostasis throughout plant development. In addition to its well-known expression root, we show that FRD3 is strongly expressed Arabidopsis thaliana seed and flower. Consistently, frd3 loss-of-function mutants are defective early germination almost completely sterile, both defects being rescued by and/or citrate supply. The fertility...
Endosomal Sorting Complex Required for Transport (ESCRT)-III proteins mediate membrane remodeling and the release of endosomal intraluminal vesicles into multivesicular bodies. Here, we show that ESCRT-III subunit paralogs CHARGED MULTIVESICULAR BODY PROTEIN1 (CHMP1A) CHMP1B are required autophagic degradation plastid in Arabidopsis thaliana. Similar to autophagy mutants, chmp1a chmp1b (chmp1) plants hyperaccumulated components, including involved division. The machinery directed bodies...
Abstract In most plant cell types, the chloroplast represents largest sink for iron, which is both essential metabolism and prone to cause oxidative damage. Here, we show that buffer potentially harmful effects of besides ferritins storage, equipped with specific iron transporters respond toxicity by removing from chloroplast. We describe two YELLOW STRIPE1-LIKE family Arabidopsis thaliana, YSL4 YSL6, are likely fulfill this function. Knocking out YSL6 greatly reduces plant's ability cope...
Summary Iron (Fe) homeostasis is crucial for all living organisms. In mammals, an integrated posttranscriptional mechanism couples the regulation of both Fe deficiency and excess responses. Whether in plants control involving common players regulates responses to still be determined. this study, molecular, genetic biochemical approaches were used investigate transcriptional excess. A activator shortage Arabidopsis, called bHLH105/ILR3, was found also negatively regulate expression ferritin...
Deciphering cellular iron (Fe) homeostasis requires having access to both quantitative and qualitative information on the subcellular pools of Fe in tissues their dynamics within cells. We have taken advantage Perls/DAB staining procedure perform a systematic analysis distribution roots, leaves reproductive organs model plant Arabidopsis thaliana, using wild-type mutant genotypes affected transport storage. Roots soil-grown plants accumulate apoplast central cylinder, pattern that is...
Abstract Mitochondrial complex II (succinate dehydrogenase) is part of the tricarboxylic acid cycle and respiratory chain. Three nuclear genes encode its essential iron-sulfur subunit in Arabidopsis (Arabidopsis thaliana). One them, SUCCINATE DEHYDROGENASE2-3 (SDH2-3), specifically expressed embryo during seed maturation, suggesting that SDH2-3 may have a role as maturation and/or germination. Here, we present data demonstrating three abscisic acid-responsive elements one RY-like enhancer...
Thermal performance curves have provided a common framework to study the impact of temperature in biological systems. However, few generalities emerged date. Here, we combine an experimental approach with theoretical analyses demonstrate that are expected vary predictably levels organization. We measured rates enzymatic reactions, organismal and population viability Drosophila acclimated different thermal conditions show become narrower optima shifting towards lower temperatures at higher or...
The molecular mechanisms by which vascular tissues acquire their identities are largely unknown. Here, we report on the identification and characterization of VASCULATURE COMPLEXITY AND CONNECTIVITY (VCC), a member 15-member, plant-specific gene family in Arabidopsis (Arabidopsis thaliana) that encodes proteins unknown function with four predicted transmembrane domains. Homozygous vcc mutants displayed cotyledon vein networks reduced complexity disconnected veins. Similar disconnections or...
Three nuclear genes, SDH2-1, SDH2-2 and SDH2-3, encode the essential iron-sulfur subunit of mitochondrial complex II in Arabidopsis thaliana. SDH2-1 probably arose via a recent duplication event we reported that both are expressed all organs from adult plants. In contrast, transcripts SDH2-3 were not detected. Here present data demonstrating is specifically during seed development. appear maturation, persist through desiccation, abundant dry seeds markedly decline germination. Analysis...
Iron is an essential micronutrient for plants. Little know about how iron loaded in embryo during seed development. In this article we used Perls/DAB staining order to reveal localization at the cellular and subcellular levels different Brassicaceae species. dry seeds of Brassica napus, Nasturtium officinale, Lepidium sativum, Camelina sativa oleracea localizes vacuoles cells surrounding provasculature cotyledons hypocotyl. Using B. napus N. officinale as model plants determined where Our...
Iron is an essential micronutrient for humans and other organisms. Its deficiency one of the leading causes anemia worldwide. The world health organization has proposed that alternative to increasing iron content in food through crop biofortification. One most consumed part crops seed, however, little known about how accumulation seed occurs it regulated. B3 transcription factors play a critical role storage compounds such as proteins lipids. Their maturation been well characterized....
Seeds accumulate iron during embryo maturation stages of embryogenesis. Using Arabidopsis thaliana as model plant, it has been described that mature embryos within a specific cell layer, the endodermis. This distribution pattern was conserved in most analyzed members from Brassicales, with exception basal Vasconcellea pubescens also showed elevated amounts cortex cells. To determine whether V. indicative wider non-Brassicales Eudicotyledoneae, we studied different belonging to plant species...
Abstract We performed a histological and quantitative study of iron in archaeological maize seeds from prehispanic times recovered Tarapacá, Atacama Desert. Also, we examined distribution changes at the cell level embryos ancient versus new varieties maize. Our results show progressive decrease concentration oldest to modern specimens. interpret as an effect agriculture over micronutrient composition
Saccharomyces cerevisiae phosphoenolpyruvate (PEP) carboxykinase catalyses the reversible metal-dependent formation of oxaloacetate and ATP from PEP, ADP, CO2 plays a key role in gluconeogenesis. This enzyme also has decarboxylase pyruvate kinase-like activities. Mutations PEP have been constructed where residues Lys213 His233, two putative Mn2+ binding site enzyme, were altered. Replacement these by Arg Gln, respectively, generated enzymes with 1.9 2.8 kcal/mol lower affinity. Lower...
Several transcription factors have been involved in the regulation of gene expression during seed development. Nutritional reserves, including iron, are principally accumulated maturation stages. Using model plant Arabidopsis thaliana, it has shown that iron is stored development vacuoles endodermis cell layer. During germination, these reserves remobilized and used by seedling heterotrophic to autotrophic metabolism switch. To date, no information about how distribution genetically...
Iron is the most abundant micronutrient in plant mitochondria and it has a crucial role biochemical reactions involving electron transfer. It been described Oryza sativa that Mitochondrial Transporter (MIT) an essential gene knockdown mutant rice plants have decreased amount of iron mitochondria, strongly suggesting OsMIT involved mitochondrial uptake. In Arabidopsis thaliana, two genes encode MIT homologues. this study, we analyzed different AtMIT1 AtMIT2 alleles, confirming individually...
Anemia due to iron deficiency is a worldwide issue, affecting mainly children and women. Seed major source of this micronutrient for feeding however in most crops these levels are too low meet daily needs. Thus, increasing allocation its storage seeds can represent an important step enhance provision humans animals. Our knowledge on seed homeostasis based studies performed the model plant Arabidopsis thaliana, where accumulates endodermis cells surrounding embryo provasculature. It has been...