A5016047491- Plant Micronutrient Interactions and Effects
- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Aluminum toxicity and tolerance in plants and animals
- Cassava research and cyanide
- Microbial Fuel Cells and Bioremediation
- Plant Stress Responses and Tolerance
- Trace Elements in Health
- Berry genetics and cultivation research
- Agronomic Practices and Intercropping Systems
- Plant-Microbe Interactions and Immunity
- Geochemistry and Elemental Analysis
- Plant Reproductive Biology
- Pharmaceutical and Antibiotic Environmental Impacts
- Mycorrhizal Fungi and Plant Interactions
- Plant Physiology and Cultivation Studies
- CRISPR and Genetic Engineering
- Rice Cultivation and Yield Improvement
- Polymer-Based Agricultural Enhancements
- Plant Virus Research Studies
- Plant responses to water stress
- bioluminescence and chemiluminescence research
- Plant Parasitism and Resistance
- Photoreceptor and optogenetics research
- Mine drainage and remediation techniques
Centre for Plant Biotechnology and Genomics
2017-2023
Universidad Politécnica de Madrid
2017-2023
Universidad Autónoma de Madrid
2010-2023
University of Oxford
2023
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2018-2023
Czech Agrifood Research Center
2009-2010
Copper is an essential nutrient for symbiotic nitrogen fixation. This element delivered by the host plant to nodule, where membrane copper (Cu) transporter would introduce it into cell synthesize cupro-proteins. COPT family members in model legume Medicago truncatula were identified and their expression determined. Yeast complementation assays, confocal microscopy phenotypical characterization of a Tnt1 insertional mutant line carried out nodule-specific M. member. genome encodes eight...
Boron (B) is an essential micronutrient for seed plants. Information on B-efficiency mechanisms and B-efficient crop model plant genotypes very scarce. Studies evaluating the basis consequences of B-deficiency are limited by facts that B occurs as a trace contaminant essentially everywhere, its bioavailability difficult to control soil-based growth systems allowing high-throughput screening populations have hitherto been lacking. The Brassica napus shows high sensitivity toward B-deficient...
Zinc is a micronutrient required for symbiotic nitrogen fixation. It has been proposed that in model legume Medicago truncatula, zinc delivered by the root vasculature into nodule and released infection/differentiation zone. There, transporters must introduce this element rhizobia-infected cells to metallate apoproteins use as cofactor. MtZIP6 (Medtr4g083570) an M. truncatula Zinc-Iron Permease (ZIP) expressed only roots nodules, with highest expression levels Immunolocalization studies...
Summary Iron is an essential cofactor for symbiotic nitrogen fixation, required by many of the enzymes involved, including signal transduction proteins, O 2 homeostasis systems, and nitrogenase itself. Consequently, host plants have developed a transport network to deliver iron nitrogen‐fixing nodule cells. Ferroportin family members in model legume Medicago truncatula were identified their expression was determined. Yeast complementation assays, immunolocalization, characterization tnt1...
Most effective nematicides for the control of root-knot nematodes are banned, which demands a better understanding plant-nematode interaction. Understanding how gene expression in nematode-feeding sites relates to morphological features may assist characterization However, nematode-induced galls resulting from cell-proliferation and hypertrophy hinders such observation, would require tissue sectioning or clearing. We demonstrate that method based on green auto-fluorescence produced by...
A characteristic feature of a plant immune response is the increase cytosolic calcium (Ca2+) concentration following infection, which results in downstream activation regulators. The bryophyte Physcomitrella patens has been shown to mount an when exposed bacteria, fungi, or chitin elicitation, manner similar one observed Arabidopsis thaliana. Nevertheless, whether P. microorganism exposure Ca2+ mediated currently unknown. Here, we show that plants treated with oligosaccharides exhibit...
The capacity to bind biomolecules is considered be the basis for any physiological role of boron (B). Legume arabinogalactan protein-extensin (AGPE), a major component infection thread matrix legume nodules potential B-ligand. Therefore, its in threads development was investigated Pisum sativum grown under B deficiency. Using AGPE-specific antibody MAC265, immunochemical analysis revealed that 175 kDa MAC265 antigen abundant +B but much weaker -B nodule extracts. A B-dependent complex...
Symbiotic nitrogen fixation carried out by the interaction between legumes and diazotrophic bacteria known as rhizobia requires relatively large levels of transition metals. These elements are cofactors many key enzymes involved in this process. Metallic micronutrients obtained from soil roots directed to sink organs vasculature, a process mediated number metal transporters small organic molecules that facilitate delivery plant fluids. Among later, nicotianamine is one most important....
Abstract Zinc is an essential nutrient at low concentrations, but toxic slightly higher ones. It has been proposed that hyperaccumulator plants may use the excess zinc to fend off pathogens and herbivores. However, there little evidence of a similar response in other plants. Here we show Arabidopsis thaliana leaves inoculated with necrotrophic fungus Plectosphaerella cucumerina BMM (PcBMM) accumulate manganese infection site. accumulation did not occur double mutant transporters HEAVY METAL...
Symbiotic nitrogen fixation carried out in legume root nodules requires transition metals. These nutrients are delivered by the host plant to endosymbiotic nitrogen-fixing bacteria living within nodule cells, a process which vascular transport is essential. As members of Yellow Stripe-Like (YSL) family metal transporters involved shoot transport, they should also be required for delivery. The genome model Medicago truncatula encodes eight YSL proteins, four them with high degree similarity...
Seeds accumulate iron during embryo maturation stages of embryogenesis. Using Arabidopsis thaliana as model plant, it has been described that mature embryos within a specific cell layer, the endodermis. This distribution pattern was conserved in most analyzed members from Brassicales, with exception basal Vasconcellea pubescens also showed elevated amounts cortex cells. To determine whether V. indicative wider non-Brassicales Eudicotyledoneae, we studied different belonging to plant species...
RNA interference (RNAi) enables flexible and dynamic interrogation of entire gene families or essential genes without the need for exogenous proteins, unlike CRISPR-Cas technology. Unfortunately, isolation plants undergoing potent silencing requires laborious design, visual screening, physical separation downstream characterization. Here, we developed an adenine phosphoribosyltransferase (APT)-based RNAi technology (APTi) in Physcomitrella patens that improves upon multiple limitations...
ABSTRACT Iron is an essential cofactor for symbiotic nitrogen fixation. It required by many of the enzymes facilitating conversion N 2 into NH 4 + endosymbiotic bacteria living within root nodule cells, including signal transduction proteins, O homeostasis systems, and nitrogenase itself. Consequently, host plants have developed a transport network to deliver iron nitrogen-fixing cells. Model legume Medicago truncatula Ferroportin2 ( MtFPN2 ) nodule-specific gene that encodes iron-efflux...