A5010005806- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Soybean genetics and cultivation
- Plant tissue culture and regeneration
- Plant-Microbe Interactions and Immunity
- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Plant Disease Resistance and Genetics
- Plant Virus Research Studies
- Photosynthetic Processes and Mechanisms
- Agronomic Practices and Intercropping Systems
- Plant Parasitism and Resistance
- Plant Disease Management Techniques
- Greenhouse Technology and Climate Control
- Hematopoietic Stem Cell Transplantation
- Plant Reproductive Biology
- Chronic Lymphocytic Leukemia Research
- Mercury impact and mitigation studies
- Acute Myeloid Leukemia Research
- Lymphoma Diagnosis and Treatment
- Algal biology and biofuel production
- Acute Lymphoblastic Leukemia research
- Heavy metals in environment
- Advanced Breast Cancer Therapies
University of Castilla-La Mancha
2015-2024
Baylor University Medical Center
2019-2024
University of Applied Sciences BFI Vienna
2024
Universität Innsbruck
2024
Kumamoto University
2019-2023
Baylor University
2021
Texas Oncology
2017-2021
Hospital Dipreca
2020
Novartis (Germany)
2019
Pontificia Universidad Católica de Valparaíso
2009-2016
Exposure of Arabidopsis plants that were maintained under low light (200 mumol photons m-2 sec-1) to excess (2000 for 1 hr caused reversible photoinhibition photosynthesis. Measurements photosynthetic parameters and the use electron transport inhibitors indicated a novel signal transduction pathway was initiated at plastoquinone regulated, least in part, by redox status pool. This signal, which preceded photooxidative burst hydrogen peroxide (H2O2) associated with photosynthesis, resulted...
Root-knot nematodes differentiate highly specialized feeding cells in roots (giant cells, GCs), through poorly characterized mechanisms that include extensive transcriptional changes. While global transcriptome analyses have used galls, which are complex root structures GCs and surrounding tissues, no gene expression changes specific to been described. We report on the differential of versus vascular induced Arabidopsis by Meloidogyne javanica at a very early stage their development, 3 days...
Summary Root‐knot nematodes ( RKN s) induce giant cells GC from root vascular inside the galls. Accompanying molecular changes as a function of infection time and across different species, their functional impact, are still poorly understood. Thus, transcriptomes tomato galls laser capture microdissected LCM ) s over course parasitism were compared with those Arabidopsis, analysis repressed gene was performed. Microarray hybridization RNA s, infection–reproduction tests quantitative reverse...
Summary Understanding the cellular mechanisms of plant tolerance to mercury ( H g) is important for developing phytoremediation strategies g‐contaminated soils. The early responses alfalfa M edicago sativa ) seedlings g were studied using transcriptomics analysis. A truncatula microarray was hybridized with high‐quality root RNA from . treated 3 μM 3, 6 and 24 h. transcriptional pattern data complementary measurements growth inhibition, lipid peroxidation, hydrogen peroxide 2 O accumulation...
Summary Root‐knot nematodes ( RKN s) induce inside the vascular cylinder giant cells GC embedded in galls. The distinctive gene repression early‐developing s could be facilitated by small RNA sRNA ) such as mi s, and/or epigenetic mechanisms mediated 24nt‐ rasi and 21‐22nt‐ s. Therefore, ‐population together with role of miR390/ TAS 3/ ARF module were studied during early gall/ formation. Three libraries from 3‐d‐post‐inoculation (dpi) galls induced Meloidogyne javanica Arabidopsis three...
The establishment of galls and syncytia as feeding sites induced by root-knot cyst nematodes, respectively, involves a progressive increase in nuclear cellular size. Here we describe the functional characterization endocycle activators CCS52A, CCS52B repressor endocycle, DEL1, during two types nematode site development Arabidopsis thaliana. In situ hybridization analysis showed that expression CCS52A1 was strongly DEL1 stably but weakly expressed throughout development. Down-regulation...
Summary Root knot nematodes ( RKN ) are root parasites that induce the genetic reprogramming of vascular cells into giant feeding and development galls. Micro RNA s (miRNAs) regulate gene expression during plant responses to various stresses. Disruption post‐transcriptional silencing in Arabidopsis ago1 or ago2 mutants decrease infection rate suggesting a role for this mechanism plant‐nematode interaction. By sequencing small from uninfected roots galls 7 14 d post with Meloidogyne incognita...
Abstract Root-knot nematodes (RKNs) are obligate endoparasites that feed on their host plants to complete its life cycle, representing a major threat agriculture and economy worldwide. The development of new management strategies becomes essential as effective chemical nematicides progressively being restricted. Hence, we analysed grape pomace-derived biochars, pyrolysed at 350 °C (BC350) 700 (BC700), focusing potential for RKN control. thermal treatment pomace caused an increase in the...
Root-knot nematodes (RKNs, Meloidogyne spp.) are obligate plant parasites that constitute a significant pest for agriculture worldwide. They penetrate the roots, reducing uptake of water and nutrients, causing impact on crop yield. One alternative focus now nematode management is biological control. Rhizobacteria within Bacillus genus show multiple modes action against plant-parasitic (PPNs) can act alone or in combination. In this context, we evaluated dual-strain bacteria combination ( B....
The responses of two closely related members Arabidopsis 13-lipoxygenases (13-LOXs), LOX3 and LOX4, to infection by the sedentary nematodes root-knot nematode (Meloidogyne javanica) cyst (Heterodera schachtii) were analysed in transgenic seedlings. tissue localization LOX4 gene expression using β-glucuronidase (GUS) reporter constructs showed local induction when second-stage juveniles reached vascular bundle during early stages plant-nematode interaction through gall syncytia formation....
Plant endoparasitic nematodes induce the formation of their feeding cells by injecting effectors from esophageal glands into root cells. Although vascular cylinder seem to be involved in root-knot nematode (RKN) structures, molecular evidence is scarce. We address role during gall development LATERAL ORGAN BOUNDARIES-DOMAIN 16 (LBD16), a key component auxin pathway leading divisions xylem pole pericycle (XPP) for lateral (LR) formation. Arabidopsis T-DNA tagged J0192 and J0121 XPP marker...
At present, there is strong commercial demand for recombinant proteins, such as antigens, antibodies, biopharmaceuticals, and industrial enzymes, which cannot be fulfilled by existing procedures. Thus, an intensive search alternative models that may provide efficiency, safety, quality control being undertaken a number of laboratories around the world. The chloroplast eukaryotic microalgae Haematococcus pluvialis Flotow has arisen candidate novel expression platform protein production....