A5031961454- Trace Elements in Health
- Tuberculosis Research and Epidemiology
- Iron Metabolism and Disorders
- Antibiotic Resistance in Bacteria
- Bacterial Genetics and Biotechnology
- Legume Nitrogen Fixing Symbiosis
- Drug Transport and Resistance Mechanisms
- Bacterial biofilms and quorum sensing
- Plant Micronutrient Interactions and Effects
- Metalloenzymes and iron-sulfur proteins
- Neurological diseases and metabolism
- Plant nutrient uptake and metabolism
- Mycobacterium research and diagnosis
- Chromium effects and bioremediation
- Electrochemical Analysis and Applications
- Escherichia coli research studies
- RNA and protein synthesis mechanisms
- Aluminum toxicity and tolerance in plants and animals
- Plant-Microbe Interactions and Immunity
- Protein Structure and Dynamics
- Signaling Pathways in Disease
- Metal Extraction and Bioleaching
- Plant responses to water stress
- Cassava research and cyanide
- Pneumocystis jirovecii pneumonia detection and treatment
Worcester Polytechnic Institute
2017-2024
National Institute of Diabetes and Digestive and Kidney Diseases
2022-2024
National Institutes of Health
2023-2024
Universidad Nacional de Colombia
2012-2019
Bacterial copper (Cu+) homeostasis enables both precise metallation of diverse cuproproteins and control variable metal levels. To this end, protein networks mobilize Cu+ to cellular targets with remarkable specificity. However, the understanding these processes is rather fragmented. Here, we use genome-wide transcriptomic analysis by RNA-Seq characterize response Pseudomonas aeruginosa external 0.5 mm CuSO4, a condition that did not generate pleiotropic effects. Pre-steady-state (5-min)...
Copper is a micronutrient required as cofactor in redox enzymes. When free, copper toxic, mismetallating proteins and generating damaging free radicals.
ABSTRACT Copper (Cu) is an essential trace element required for respiration, neurotransmitter synthesis, oxidative stress response, and transcriptional regulation. Imbalance in Cu homeostasis can lead to several pathological conditions, affecting neuronal, cognitive, muscular development. Mechanistically, Cu-binding proteins (Cu-BPs) have important but underappreciated role transcription regulation mammalian cells. In this context, our lab investigates the contributions of novel Cu-BPs...
P-type ATPases hydrolyze ATP and release energy that is used in the transport of ions against electrochemical gradients across plasma membranes, making these proteins essential for cell viability. Currently, distribution function ion transporters mycobacteria are poorly understood.In this study, probabilistic profiles were constructed based on hidden Markov models to identify classify Mycobacterium tuberculosis complex (MTBC) according type transported membrane. Topology, hydrophobicity...
Among the 12 P-type ATPases encoded by genome of
Symbiotic nitrogen fixation carried out by the interaction between legumes and diazotrophic bacteria known as rhizobia requires relatively large levels of transition metals. These elements are cofactors many key enzymes involved in this process. Metallic micronutrients obtained from soil roots directed to sink organs vasculature, a process mediated number metal transporters small organic molecules that facilitate delivery plant fluids. Among later, nicotianamine is one most important....
Symbiotic nitrogen fixation carried out in legume root nodules requires transition metals. These nutrients are delivered by the host plant to endosymbiotic nitrogen-fixing bacteria living within nodule cells, a process which vascular transport is essential. As members of Yellow Stripe-Like (YSL) family metal transporters involved shoot transport, they should also be required for delivery. The genome model Medicago truncatula encodes eight YSL proteins, four them with high degree similarity...
Iron-sulfur (Fe-S) clusters are essential cofactors found in many proteins the mitochondria, cytosol, and nucleus of cell. These versatile may undergo reversible oxidation-reduction reactions to enable electron transfers; they be structural confer stability a folded protein; regulatory transduce an iron signal that alters function or recipient protein. Of nearly 70 described mammalian cells bind Fe-S clusters, about half localize exclusively partially nucleus, where required for DNA...
Copper (Cu) is essential for respiration, neurotransmitter synthesis, oxidative stress response, and transcription regulation, with imbalances leading to neurological, cognitive, muscular disorders. Here we show the role of a novel Cu-binding protein (Cu-BP) in mammalian transcriptional specifically on skeletal muscle differentiation using murine primary myoblasts. Utilizing synchrotron X-ray fluorescence-mass spectrometry, identified cysteine-rich intestinal 2 (mCrip2) as key Cu-BP abundant...
Iron–sulfur (Fe-S) clusters are essential cofactors found in many proteins the mitochondria, cytosol, and nucleus of cell. These versatile may undergo reversible oxidation–reduction reactions to enable electron transfers; they be structural confer stability a folded protein; regulatory transduce an iron signal that alters function or recipient protein. Of nearly 70 described mammalian cells bind Fe-S clusters, about half localize exclusively partially nucleus, where required for DNA...
SUMMARY Symbiotic nitrogen fixation carried out by the interaction between legumes and diazotrophic bacteria known as rhizobia requires of relatively large levels transition metals. These elements act cofactors many key enzymes involved in this process. Metallic micronutrients are obtained from soil roots directed to sink organs vasculature, a process participated number metal transporters small organic molecules that mediate delivery plant fluids. Among later, nicotianamine is one most...
Bacterial Cu + homeostasis is based on protein networks that mobilize copper to cellular targets, enabling both metallation of cuproproteins and maintenance non‐toxic metal levels. Here, we use genome wide expression analysis (RNA‐Seq) characterize the response Pseudomonas aeruginosa external 0.5 mM CuSO 4 , a condition where cell viability uncompromised. Analysis copR cueR mutant strains revealed compartmental levels are independently controlled; cytoplasmic sensor CueR regulates chaperones...
Copper is a micronutrient required by multiple enzymes as redox co‐factor. However, free Cu toxic, leading to Fe‐S cluster disassembly and reactive oxygen species. As such, immune cells use phagosomal Cu‐overload clear pathogens. Thus, bacteria need direct correct targets while maintaining non‐toxic levels. To limit toxicity, sequester drive the efflux of using various mechanisms. Among these, single cytoplasmic Cu‐chaperones mobilize metal within cell compartments. We recently used RNA‐seq...
Abstract Symbiotic nitrogen fixation carried out in legume root nodules requires transition metals. These nutrients are delivered by the host plant to endosymbiotic nitrogen-fixing bacteria living with nodule cells, a process which vascular transport is essential. As occurs root-to-shoot transport, members of Yellow Stripe-Like (YSL) family metal transporters should also be required for root-to-nodule delivery. The genome model Medicago truncatula encodes eight YSL proteins, four them high...
ABSTRACT Two component systems control periplasmic Cu + homeostasis in Gram-negative bacteria. In characterized such as Escherichia coli CusRS, upon binding to the sensing domain of CusS, a cytoplasmic phosphotransfer phosphorylates response regulator CusR. This drives expression efflux transporters, chaperones, and redox enzymes ameliorate metal toxic effects. Here, we show that Pseudomonas aeruginosa two sensor histidine kinase CopS exhibits Cu-dependent phosphatase activity maintains...