A5037042657- Plant Micronutrient Interactions and Effects
- Plant Stress Responses and Tolerance
- Plant nutrient uptake and metabolism
- Aluminum toxicity and tolerance in plants and animals
- Horticultural and Viticultural Research
- Molecular Biology Techniques and Applications
- Trace Elements in Health
- Legume Nitrogen Fixing Symbiosis
- RNA modifications and cancer
- Plant Gene Expression Analysis
- Plant Pathogens and Fungal Diseases
- Plant and Fungal Interactions Research
- Bacterial biofilms and quorum sensing
- Sugarcane Cultivation and Processing
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Plant-Microbe Interactions and Immunity
- Ecology and Conservation Studies
- Electrochemical Analysis and Applications
- Nuts composition and effects
- Heavy Metal Exposure and Toxicity
- Single-cell and spatial transcriptomics
- Natural Products and Biological Research
- Biofuel production and bioconversion
- Vibrio bacteria research studies
Universidad Politécnica de Madrid
2014-2023
Centre for Plant Biotechnology and Genomics
2014-2023
Universidad Rey Juan Carlos
2023
Ruhr University Bochum
2019-2022
Worcester Polytechnic Institute
2016-2021
University of Helsinki
2015
Bacterial copper (Cu+) homeostasis enables both precise metallation of diverse cuproproteins and control variable metal levels. To this end, protein networks mobilize Cu+ to cellular targets with remarkable specificity. However, the understanding these processes is rather fragmented. Here, we use genome-wide transcriptomic analysis by RNA-Seq characterize response Pseudomonas aeruginosa external 0.5 mm CuSO4, a condition that did not generate pleiotropic effects. Pre-steady-state (5-min)...
SUMMARY IRON‐REGULATED TRANSPORTER1 (IRT1) is the root high‐affinity ferrous iron (Fe) uptake system and indispensable for completion of life cycle Arabidopsis thaliana without vigorous Fe supplementation. Here we provide evidence supporting a second role IRT1 in root‐to‐shoot partitioning Fe. We show that irt1 mutants overaccumulate roots, most prominently cortex differentiation zone ‐2, compared to wild type. Shoots ‐2 are severely Fe‐deficient according content marker transcripts, as...
Summary Iron is an essential cofactor for symbiotic nitrogen fixation, required by many of the enzymes involved, including signal transduction proteins, O 2 homeostasis systems, and nitrogenase itself. Consequently, host plants have developed a transport network to deliver iron nitrogen‐fixing nodule cells. Ferroportin family members in model legume Medicago truncatula were identified their expression was determined. Yeast complementation assays, immunolocalization, characterization tnt1...
Abstract Diphthamide, a post-translationally modified histidine residue of eukaryotic TRANSLATION ELONGATION FACTOR2 (eEF2), is the human host cell-sensitizing target diphtheria toxin. Diphthamide biosynthesis depends on 4Fe-4S-cluster protein Dph1 catalyzing first committed step, as well Dph2 to Dph7, in yeast and mammals. Here we show that diphthamide modification eEF2 conserved Arabidopsis thaliana requires AtDPH1. Ribosomal −1 frameshifting-error rates are increased dph1 mutants, similar...
Zinc transporters facilitate metal mobilization and compartmentalization, playing a key role in cellular development. Little is known about the mechanisms pathways of Zn movement between metalloproteins during myoblast differentiation. We analyzed differential expression ZIP ZnT C2C12 account for transient decrease intracellular upon myogenesis induction followed by gradual increase myotubes. Considering subcellular localization function each transporters, our findings indicate that fine...
Copper (Cu) is a cofactor of around 300 Arabidopsis proteins, including photosynthetic and mitochondrial electron transfer chain enzymes critical for adenosine triphosphate (ATP) production carbon fixation. Plant acclimation to Cu deficiency requires the transcription factor SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE7 (SPL7). We report that in wild type (WT) spl7-1 mutant, respiratory flux via Cu-dependent cytochrome c oxidase unaffected under both normal low-Cu cultivation conditions....
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Biological systems require precise copper homeostasis enabling metallation of cuproproteins while preventing metal toxicity. In bacteria, sensing, transport, and storage molecules act in coordination to fulfill these roles. However, there is not yet a kinetic schema explaining the system integration. Here, we report model emerging from experimental computational approaches that describes dynamics distribution Pseudomonas aeruginosa. Based on uptake experiments, minimal well wild-type...
Abstract Yellow Stripe‐Like (YSL) proteins are a family of plant transporters that typically involved in transition metal homeostasis. Three the four YSL clades (I, II and IV) transport metals complexed with non‐proteinogenic amino acid nicotianamine or its derivatives. No such capability has been shown for any member clade III, but link between these YSLs homeostasis could be masked by functional redundancy. We studied role III protein MtSYL7 Medicago truncatula nodules. MtYSL7, which...
The advances in high-throughput sequencing technologies are allowing more and de novo assembling of transcriptomes from many new organisms. Some degree automation evaluation is required to warrant reproducibility, repetitivity the selection best possible transcriptome. Workflows pipelines becoming an absolute requirement for such a purpose, but issue organisms lacking sequenced genome remains unsolved. An automated, reproducible flexible framework called TransFlow accomplish this task...
Heavy metal-rich toxic soils and ordinary are both natural habitats of Arabidopsis halleri, a diploid perennial obligate outcrosser in the sister clade genetic model plant thaliana. The molecular divergence underlying survival sharply contrasting environments is unknown. Here we comparatively address metal physiology transcriptomes A. halleri originating from most highly heavy metal-contaminated soil Europe, Ponte Nossa, Italy (Noss), non-metalliferous (NM) soils. Plants Noss exhibit...
Mixed saliva from two caries-free individuals possessed antimicrobial activity toward Streptococcus mutans and S. sanguis. This inhibitory was attributed to the presence of a group four anionic proteins each which strongly inhibited growth oral streptococci in protein-based medium but not containing amino acids as nitrogen source. These proteins, with isoelectric points 4.70, 4.90, 4.98, 5.05, respectively, neither reacted antisera immunoglobulin A, G, or M nor appeared be functionally...
ABSTRACT Iron is an essential cofactor for symbiotic nitrogen fixation. It required by many of the enzymes facilitating conversion N 2 into NH 4 + endosymbiotic bacteria living within root nodule cells, including signal transduction proteins, O homeostasis systems, and nitrogenase itself. Consequently, host plants have developed a transport network to deliver iron nitrogen-fixing cells. Model legume Medicago truncatula Ferroportin2 ( MtFPN2 ) nodule-specific gene that encodes iron-efflux...
ABSTRACT IRON-REGULATED TRANSPORTER1 (IRT1) is the root high-affinity ferrous iron uptake system and indispensable for completion of life cycle Arabidopsis thaliana without vigorous (Fe) supplementation. Here we provide evidence supporting a second role IRT1 in root-to-shoot mobilization Fe. We show that irt1 -2 ( pam42 ) mutant over-accumulates Fe roots, most prominently cortex differentiation zone, when compared to wild type. Shoots are severely Fe-deficient according content marker...
ABSTRACT Yellow Stripe-Like (YSL) proteins are a family of plant transporters typically involved in transition metal homeostasis. The substrate three the four YSL clades (clades I, II, and IV) complexes with non-proteinogenic amino acid nicotianamine or its derivatives. No such transport capabilities have been shown for any member remaining clade (clade III), which is able to translocate short peptides across membranes instead. connection between III members homeostasis might masked by...
Aim of the study: The research was aimed to design microsatellite markers for genotyping and differentiation trees from walnut hybrid progeny Mj209×Ra. As a secondary objective, transferability classificatory capacity some these loci were assessed Juglans genus.Area most widely spread used in Europe wood production used. Pure species genus as Arizona black (J. major (Torrey) Heller) European or common regia L.), well different Mj209xRa lots, also included.Materials methods: Genomic DNA tree...
Abstract Heavy metal-rich toxic soils and ordinary are both natural habitats of Arabidopsis halleri . The molecular divergence underlying survival in sharply contrasting environments is unknown. Here we comparatively address metal physiology transcriptomes A. originating from the most highly heavy metal-contaminated soil Europe, Ponte Nossa (Noss/IT), non-metalliferous (NM) soil. Noss exhibits enhanced hypertolerance attenuated accumulation cadmium (Cd), transcriptomic Cd responsiveness...
Prunus genus include fruit and wooden species. Because its economic importance, both phenotypic genetic markers have been developed used in studies. Among them, for their versatility, degree of transferability repeatability, some microsatellite sets several species from this genus.Although they successfully inside the Rosaceae family, to other and/or provenances different what were designed, must be first assessed. Thus, higher number assessed, applicability performing Hence, functionality...