A5040291886- Plant Micronutrient Interactions and Effects
- Plant Stress Responses and Tolerance
- Banana Cultivation and Research
- Aluminum toxicity and tolerance in plants and animals
- Plant Physiology and Cultivation Studies
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Legume Nitrogen Fixing Symbiosis
- Catalytic Alkyne Reactions
- Plant nutrient uptake and metabolism
- Synthetic Organic Chemistry Methods
- Analytical chemistry methods development
- Iron oxide chemistry and applications
- Horticultural and Viticultural Research
- Asymmetric Synthesis and Catalysis
- Mercury impact and mitigation studies
- Agricultural Science and Fertilization
- Postharvest Quality and Shelf Life Management
- Heavy metals in environment
- Heavy Metals in Plants
- Cassava research and cyanide
- Cyclopropane Reaction Mechanisms
- Ion Channels and Receptors
- Nutrition, Genetics, and Disease
- Phytase and its Applications
Estación Experimental de Aula Dei
2014-2024
Consejo Superior de Investigaciones Científicas
2013-2023
Experimental Station
2016-2020
Universidad de Oviedo
2009-2014
Institut Català d'Investigació Química
2013
In-Q-Tel
2010
National Research Council
2007
Centro de Investigación y Tecnología Agroalimentaria de Aragón
2006-2007
Centre National de la Recherche Scientifique
2005
Universidad Autónoma de Madrid
1996-2004
Summary Studies of Iron (Fe) uptake mechanisms by plant roots have focussed on Fe( III )‐siderophores or II ) transport systems. deficency also enhances root secretion flavins and phenolics. However, the nature these compounds, their outside role in Fe nutrition are largely unknown. We used HPLC / ESI ‐ MS ( TOF (ion trap) to characterize fluorescent phenolic‐type compounds accumulated exported culture medium Arabidopsis plants response deficiency. Wild‐type mutant altered either...
The identification of Fe transport forms in plant xylem sap is crucial to the understanding long-distance processes plants. Previous studies have proposed that may be transported as an Fe–citrate complex sap, but such a has never been detected. In this study we report first direct and unequivocal natural sap. A tri-Fe(III), tri-citrate (Fe3Cit3) was found Fe-deficient tomato (Solanum lycopersicum Mill. cv. 'Tres Cantos') resupplied with Fe, by using integrated mass spectrometry approach...
Root secretion of coumarin-phenolic type compounds has been recently shown to be related Arabidopsis thaliana tolerance Fe deficiency at high pH. These studies revealed the identity a few simple coumarins occurring in roots and exudates Fe-deficient A. plants, left open possible existence other unknown phenolics. We used HPLC-UV-VIS-ESI-MS(TOF), HPLC/ESI-MSn(ion trap) HPLC-ESI-MS-MS(Q-TOF) characterize (identify quantify) phenolic-type accumulated or secreted into nutrient solution plants...
Nicotianamine (NA) and 2'-deoxymugenic acid (DMA) are metal-chelating ligands that promote the accumulation of metals in rice endosperm, but it is unclear how these phytosiderophores regulate levels different limit their accumulation. In this study, transgenic plants producing high NA DMA accumulated up to 4-fold more iron (Fe) 2-fold zinc (Zn) endosperm compared with wild-type plants. The distribution Fe Zn vegetative tissues suggested both sequestered as a buffering mechanism avoid...
A simple, highly selective, sensitive, and reproducible liquid chromatography−electrospray ionization/time-of-flight mass spectrometry method has been developed for the direct simultaneous determination of capsaicin dihydrocapsaicin in Capsicum fruit extracts. Capsaicin are two major members so-called capsaicinoid family, which includes other minor analogues, usually account at least 90% pungency trait fruits. Chromatographic separation was achieved with a reversed-phase chromatography...
Abstract Nicotianamine (NA) is considered as a key element in plant metal homeostasis. This non‐proteinogenic amino acid has an optimal structure for chelation of ions, with six functional groups that allow octahedral coordination. The ability to chelate metals by NA largely dependent on the pK resulting complex and pH solution, most being chelated at neutral or basic values. In silico calculations using pKa values have predicted occurrence metal‐NA complexes fluids, but use soft ionization...
Plants grown under iron deficiency show different morphological, biochemical and physiological changes. These changes include, among others, the elicitation of strategies to improve acquisition Fe from rhizosphere, adjustment homeostasis processes a reorganization carbohydrate metabolism. The application modern techniques that allow simultaneous untargeted analysis multiple proteins metabolites can provide insight into taking place in plants deficiency. objective this study was characterize...
Three-week-old alfalfa (Medicago sativa), barley (Hordeum vulgare) and maize (Zea mays) were exposed for 7 d to 30 µm of mercury (HgCl(2) ) characterize the Hg speciation in root, with no symptoms being poisoned. The largest pool (99%) was associated particulate fraction, whereas soluble fraction (SF) accounted a minor proportion (<1%). Liquid chromatography coupled electro-spray/time flight mass spectrometry showed that bound an array phytochelatins (PCs) root SF, which particularly varied...
Iron (Fe) is abundant in soils but generally poorly soluble. Plants, with the exception of Graminaceae, take up Fe using an Fe(III)-chelate reductase coupled to Fe(II) transporter. Whether or not nongraminaceous species can convert scarcely soluble Fe(III) forms into has deserved little attention so far. We have used Beta vulgaris, one among many whose roots secrete flavins upon deficiency, study whether are involved acquisition. Flavins secreted by Fe-deficient plants were removed from...
Summary Many metal transporters in plants are promiscuous, accommodating multiple divalent cations including some which toxic to humans. Previous attempts increase the iron (Fe) and zinc (Zn) content of rice endosperm by overexpressing different have therefore led unintentionally accumulation copper (Cu), manganese (Mn) cadmium (Cd). Unlike other transporters, barley Yellow Stripe 1 (Hv YS 1) is specific for Fe. We investigated mechanistic basis this preference constitutively expressing Hv...
Iron and Zn deficiencies are worldwide nutritional disorders that can be alleviated by increasing the metal concentration of rice (Oryza sativa L.) grains via bio-fortification approaches. The overproduction chelator nicotianamine (NA) is among most effective ones, but it still unclear whether this due to enrichment in NA itself and/or concomitant derivative 2'-deoxymugineic acid (DMA). endosperm commonly consumed portion grain mediates transfer nutrients from vegetative tissues rich embryo....
Iron homeostasis is an important process for flower development and plant fertility. The role of plastids in these processes has been shown to be essential. To document the relationships between plastid iron biology further, a global study (transcriptome, proteome, metabolome, hormone analysis) was performed Arabidopsis flowers from wild-type triple atfer1-3-4 ferritin mutant plants grown under iron-sufficient or excess conditions. Some major modifications specific functional categories were...
High soil carbonate limits crop performance especially in semiarid or arid climates. To understand how plants adapt to such soils, we explored natural variation tolerance small local populations (demes) of Arabidopsis thaliana growing on soils differing content. Reciprocal field-based transplants with elevated (+C) and without (-C) over several years revealed that demes native showed higher fitness than those when both were grown together carbonate-rich soil. This supports the role as a...
The effects of iron (Fe) deficiency on fruit yield and quality were measured in two peach cultivars, Carson (yellow-skin fruit) Babygold (red-skin fruit). In both Fe caused major decreases fresh weight per tree number fruits tree. Fruits from Fe-deficient trees had a smaller size, resulting large decrease the percentage commercially acceptable fruits, whereas firmness was unaffected. cv. Babygold, greatly decreased red color skin. Part these results likely associated with delay ripening....
The root accumulation and excretion of riboflavin (Rbfl) Rbfl derivatives have been studied in the model legume species Medicago truncatula, grown hydroponics two different Fe deficiency conditions, with without CaCO3. Using high resolution mass spectrometry techniques coupled to liquid chromatography, three flavin not previously reported plants, putatively identified as 7-hydroxy-Rbfl, 7α-hydroxy-Rbfl 7-carboxy-Rbfl, were found along Fe-deficient M. truncatula roots. In presence CaCO3 most...
Abstract The objective of this paper is to review the developments in last few years two important issues related Fe deficiency plants. First, current knowledge on possible ways carry out diagnosis and prognosis plants discussed. This includes discussion best a meaningful analysis Fe-containing compounds different plant parts. We will also discuss other measurement techniques that can permit assess nutritional status plants, including leaf chlorophyll concentrations others. Second, new...