High soil carbonate limits crop performance especially in semiarid or arid climates. To understand how plants adapt to such soils, we explored natural variation tolerance small local populations (demes) of Arabidopsis thaliana growing on soils differing content. Reciprocal field-based transplants with elevated (+C) and without (-C) over several years revealed that demes native showed higher fitness than those when both were grown together carbonate-rich soil. This supports the role as a...

High bicarbonate concentrations of calcareous soils with high pH can affect crop performance due to different constraints. Among these, Fe deficiency has mostly been studied. The ability mobilize sparingly soluble is a key factor for tolerance. Here, comparative transcriptomic analysis was performed two naturally selected Arabidopsis thaliana demes, the carbonate-tolerant A1(c+) and sensitive T6(c−). Analyses plants exposed either stress alone (pH 5.9 vs. 8.3) or alkalinity caused by 10 mM...

Abstract Purpose Alkaline salinity constrains crop yield. Previously, we observed local adaptation of Arabidopsis thaliana to saline-siliceous soils (pH ≤ 7) and non-saline carbonate soils. However, no natural population A. was localized on saline-alkaline This suggests that tolerance evolved may not confer alkaline salinity. hypothesis explored by addressing physiological molecular responses differ in either non-alkaline or carbonate. Methods native (high salinity, HS), alkalinity, HA),...

Noccaea brachypetala is a close relative of caerulescens , model plant species used in metal hyperaccumulation studies. In previous survey the Catalan Pyrenees, we found two occidental and oriental N. populations growing on non-metalliferous soils, with accumulated high concentrations Cd Zn. Our hypothesis was that microbiome companion roots may influence ability these plants to absorb metals. We performed high-throughput sequencing bacterial fungal communities rhizosphere soil rhizoplane...

Soil contamination by lead (Pb) has become one of the major ecological threats to environment. Understanding mechanisms Pb transport and deposition in plants is great importance achieve a global reduction. We exposed collection 360 Arabidopsis thaliana natural accessions Pb-polluted soil. Germination rates, growth, leaf concentrations showed extensive variation among accessions. These phenotypic data were subjected genome wide association studies (GWAs) we found significant on chromosome 1...

In nature, multiple stress factors occur simultaneously. The screening of natural diversity panels and subsequent Genome-Wide Association Studies (GWAS) is a powerful approach to identify genetic components various responses. Here, the nutritional status variation set 270 accessions Arabidopsis thaliana grown on saline-carbonated soil evaluated. We report significant leaf Na (LNa) Fe (LFe) concentrations in studied accessions. Allelic NINJA YUC8 genes associated with LNa diversity, ALA3 LFe...

In the context of current climate change, alkaline salinity is increasingly challenging crop yields, especially in arid and semiarid regions. As 98% plants are glycophytes, study aims to identify physiological genetic traits underlying differential tolerance by exploiting natural variation responses Arabidopsis thaliana local populations (demes) neutral salinity. Growth, photosynthesis, phytohormone mineral nutrient profiles, transcriptomic changes were analyzed four demes with contrasted...

Carbonic anhydrases (CAs) are the main enzymes handling bicarbonate in different cell compartments. This study analyses expression of CAs roots Arabidopsis thaliana demes differing tolerance to bicarbonate: tolerant A1(c+) deme and sensitive deme, T6(c-). While 10 mM NaCl caused a transient depolarization root membranes, NaHCO3 hyperpolarization. hyperpolarization was much stronger than Acetazolamide (AZ), specific inhibitor CAs, abolished hyperpolarizing effect A1(c+), indicating...

Abstract Carbonic anhydrases (CAs) are the main enzymes handling bicarbonate in different cell compartments. This study analyses expression of CAs roots Arabidopsis thaliana demes differing tolerance to bicarbonate: tolerant A1 (C+) deme and sensitive deme, T6 (C‐) . Exposure 10 mM NaCl caused a transient depolarization root membranes, contrast, supply NaHCO 3 hyperpolarization. hyperpolarization was much stronger than Acetazolamide (AZ), specific inhibitor CAs, abolished hyperpolarizing...

Carbonate-rich soils limit plant performance and crop production. Previously, local adaptation to carbonated was detected in wild Arabidopsis thaliana accessions, allowing the selection of two demes with contrasting phenotypes: A1 (carbonate tolerant, c+) T6 sensitive, c-). Here, parental lines F3 population derived from their crossing were cultivated on soil evaluate growth, fitness, segregation pattern progeny. To understand genetic architecture beyond contrasted phenotypes a bulk...

Carbonate-rich soils limit plant performance and crop production. Previously, local adaptation to carbonated was detected in wild Arabidopsis thaliana accessions, allowing the selection of two demes with contrasting phenotypes: A1 (carbonate tolerant, c+) T6 sensitive, c-). Here, seedlings were grown hydroponically under control (pH 5.9) bicarbonate conditions (10 mM NaHCO , pH 8.3) obtain ionomic profiles conduct transcriptomic analysis. In parallel, parental lines their progeny cultivated...

Abstract More than 70% of land’s cultivated area is affected by alkaline salinity stress. As 98% plants are glycophytes – unable to successfully reproduce under our previous research focused on comparative studies Arabidopsis thaliana demes with differential performance neutral and (neuSAL alkSAL ) due local adaptation processes. Here, an integrated analysis leaf tissue was performed, including physiological indicators, nutritional status, endogenous phytohormonal concentration transcriptome...

Abstract Purpose Alkaline salinity constrains crop yield. Previously, we found local adaptation of Arabidopsis thaliana demes to saline-siliceous soils (pH≤7) and non-saline carbonate soils. However, any natural population A. was localized on saline-alkaline This suggests that tolerance evolved may not confer alkaline salinity. hypothesis explored by addressing physiological molecular responses conditions differing in tolerance. Methods native (G3), (G1), or with intermediate levels these...