A5012781343- Trace Elements in Health
- Plant Micronutrient Interactions and Effects
- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Plant Stress Responses and Tolerance
- Iron Metabolism and Disorders
- Heavy Metal Exposure and Toxicity
- Genetic Neurodegenerative Diseases
- Metalloenzymes and iron-sulfur proteins
- Metal complexes synthesis and properties
- Environmental Toxicology and Ecotoxicology
- Fungal and yeast genetics research
- Plant nutrient uptake and metabolism
- Phytase and its Applications
- Heavy metals in environment
- Postharvest Quality and Shelf Life Management
- Porphyrin Metabolism and Disorders
- Celiac Disease Research and Management
- Prion Diseases and Protein Misfolding
- Plant responses to water stress
- Nitrogen and Sulfur Effects on Brassica
- Silicon Effects in Agriculture
- Plant Pathogenic Bacteria Studies
- Forest Ecology and Biodiversity Studies
- RNA Research and Splicing
Centro de Estudios Fotosintéticos y Bioquímicos
2014-2023
Consejo Nacional de Investigaciones Científicas y Técnicas
2014-2023
National University of Rosario
2010-2023
Centro Científico Tecnólogico - Rosario
2020
Universitat de Barcelona
2005-2014
Molina Center for Energy and the Environment
2006-2013
National University of the Littoral
2009
Institut de Biomedicina de la Universitat de Barcelona
2009
Instituto de Desarrollo Tecnológico para la Industria Química
2009
Abstract Background The degree of metal binding specificity in metalloproteins such as metallothioneins (MTs) can be crucial for their functional accuracy. Unlike most other animal species, pulmonate molluscs possess homometallic MT isoforms loaded with Cu + or Cd 2+ . They have, so far, been obtained native metal-MT complexes from snail tissues, where they are involved the metabolism ion species bound to respective isoform. However, it has not yet discerned if specific occupation is result...
Zinc is an essential metal that, when in excess, can be deleterious to the cell. Therefore, homeostatic mechanisms for this cation must finely tuned. To better understand response of yeast front excess zinc, we screened a systematic deletion mutant library altered growth presence 6 mM zinc. Eighty-nine mutants exhibited increased zinc sensitivity, including many genes involved vacuolar assembling and biogenesis. Interestingly, lacking Aft1 transcription factor, required transcriptional iron...
Summary Crs5 is a Saccharomyces cerevisiae Metallothionein (MT), non‐homologous to the paradigmatic Cu‐thionein Cup1. Although considered secondary copper‐resistance agent, we show here that it determines survival under zinc overload in CUP1 ‐null background. Its overexpression prevents deleterious effects exhibited by CUP1‐CRS5 cells when exposed combined Zn/Cu, as does mouse MT1 Zn‐thionein, but not The detailed characterization of vivo and vitro Zn(II)‐, Cd(II)‐ Cu(I)‐binding abilities...
A sulfide surprise: The analysis of ZnII–, CdII–, and CuI–MT recombinant preparations metallothioneins (MTs) from many organisms provides clear evidence that ions are present in nearly all their physiologically stable ZnII–MT CdII–MT complexes (see picture), but none the species. features MT good correlation with those reported for plant yeast ZnII–or CdII–γ-glutamyl peptides. Supporting information this article is available on WWW under...
Frataxin is a highly-conserved protein from prokaryotes to eukaryotes. Several functions related iron metabolism have been postulated for this protein, including Fe-S cluster and heme synthesis, response oxidative damage phosphorylation. In plants, the presence of one or two isoforms with dual localization in mitochondria chloroplasts has reported. deficiency affects both organelles, leading an impairment mitochondrial respiration, chlorophyll photosynthetic electron transport chloroplasts....
It has previously been shown that recombinant synthesis, under metal-supplemented conditions, of diverse metallothioneins (MTs) results in the recovery a subpopulation S(2-)-containing complexes addition to S(2-)-devoid canonical metal-MT species. Further significance this finding remained veiled by possibility it being mere consequence synthesis heterologous bacterial system. Herein, we present definitive evidence S(2-) ligands are also constituents native complexes. Because, although...
In acidic soils, aluminum (Al) toxicity is a significant limitation to crop production worldwide. Given its Al-binding capacity, malate allows internal as well external detoxification strategies cope with Al stress, but little known about the metabolic processes involved in this response. Here, we analyzed relevance of NADP-dependent malic enzyme (NADP-ME), which catalyzes oxidative decarboxylation malate, tolerance. Plants lacking NADP-ME1 (nadp-me1) display reduced inhibition root...
In plants, the cysteine desulfurase (AtNFS1) and frataxin (AtFH) are involved in formation of Fe-S groups mitochondria, specifically, Fe sulfur loading onto scaffold proteins, subsequent mature cluster. We found that small mitochondrial chaperone, AtISD11, AtFH positive regulators for AtNFS1 activity Arabidopsis. Moreover, when three proteins were incubated together, a stronger attenuation Fenton reaction was observed compared to with alone. Using pull-down assays, we these physically...
The Saccharomyces cerevisiae gene PIF1 encodes a conserved eukaryotic DNA helicase required for both mitochondrial and nuclear integrity. Our previous work revealed that pif1Δ strain is tolerant to zinc overload. In the present study we demonstrate this effect independent of Pif1 activity only observed when protein absent from mitochondria. cells accumulate abnormal amounts iron. Transcriptional profiling reveals under standard growth conditions overexpress aconitase-related genes. When...
Abstract Metallothioneins achieve metal binding specificity by modulation of their amino acid sequences through evolution. Non‐coordinating residues seem to play a key role in this function, and among them histidine may be particular importance. Here we report how residue regulates Cu I highly copper specific isoform, the CuMT snail Helix pomatia , analysis recombinant complexes yielded constructed mutant where has been changed an alanine.