A5085780942- Plant Micronutrient Interactions and Effects
- Probiotics and Fermented Foods
- Plant Stress Responses and Tolerance
- Microbial Metabolites in Food Biotechnology
- Plant responses to water stress
- Antimicrobial Peptides and Activities
- Transgenic Plants and Applications
- Plant nutrient uptake and metabolism
- Enzyme Production and Characterization
- Botanical Research and Chemistry
- Gut microbiota and health
- Legume Nitrogen Fixing Symbiosis
- Agricultural Science and Fertilization
- Potato Plant Research
- Growth and nutrition in plants
- Reservoir Engineering and Simulation Methods
- Milk Quality and Mastitis in Dairy Cows
- Autophagy in Disease and Therapy
- Seed Germination and Physiology
- Geological and Geophysical Studies Worldwide
- Plant Molecular Biology Research
- Geological Modeling and Analysis
- Soil Management and Crop Yield
- Plant Virus Research Studies
- Plant Disease Resistance and Genetics
Institut des Sciences des Plantes de Montpellier
2016-2021
Centre National de la Recherche Scientifique
2008-2020
Institut Agro Montpellier
2019-2020
Université de Montpellier
2019-2020
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020
Protéomique, Réponse Inflammatoire et Spectrométrie de Masse
2016
Institut National de la Recherche Agronomique
2014
La Rochelle Université
2012
Université de Strasbourg
2009-2011
Laboratoire de Chimie
2008
Iron is one of the most important micronutrients in plants as it involved many cellular functions (e.g., photosynthesis and respiration). Any defect iron availability will affect plant growth development well crop yield product quality. Thus, homeostasis must be tightly controlled order to ensure optimal absorption this mineral element. Understanding mechanisms governing has been focus several studies during past 10 years. These have greatly improved our understanding involved, revealing a...
Iron (Fe) is an essential micronutrient for plant growth and development. Any defects in the maintenance of Fe homeostasis will alter productivity quality their derived products. In Arabidopsis (Arabidopsis thaliana), transcription factor ILR3 plays a central role controlling homeostasis. this study, we identified bHLH121 as ILR3-interacting factor. Interaction studies showed that also interacts with three closest homologs (i.e., basic-helix-loop-helix 34 [bHLH34], bHLH104, bHLH115). bhlh121...
Abstract Background Lactic acid bacteria are commonly marketed as probiotics based on their putative or proven health-promoting effects. These effects known to be strain specific but the underlying molecular mechanisms remain poorly understood. Therefore, unravelling determinants behind probiotic features is of particular interest since it would help select strains that stand best chance success in clinical trials. Bile tolerance one most crucial properties determines ability survive small...
Summary Iron (Fe) homeostasis is crucial for all living organisms. In mammals, an integrated posttranscriptional mechanism couples the regulation of both Fe deficiency and excess responses. Whether in plants control involving common players regulates responses to still be determined. this study, molecular, genetic biochemical approaches were used investigate transcriptional excess. A activator shortage Arabidopsis, called bHLH105/ILR3, was found also negatively regulate expression ferritin...
RNA silencing is a major antiviral defense mechanism in plants and invertebrates. Plant ARGONAUTE1 (AGO1) pivotal silencing, hence target for counteracting viral suppressors of RNA-silencing proteins (VSRs). P0 from Turnip yellows virus (TuYV) VSR that was previously shown to trigger AGO1 degradation via an autophagy-like process. However, the identity host involved cellular site at which interact were unknown. Here we report associate on endoplasmic reticulum (ER), resulting their loading...
Summary Iron (Fe) is a major micronutrient and required for plant growth development. Nongrass species have evolved reduction‐based strategy to solubilize take up Fe. The secretion of Fe‐mobilizing coumarins (e.g. fraxetin, esculetin sideretin) by roots plays an important role in this process. Although the biochemical mechanisms leading their biosynthesis been well described, very little known about cellular subcellular localization or mobility within tissues. Spectral imaging was used...
The identification of cell components involved in probiotic activities is a challenge current research. In this work, new approach based on proteomics as an analytical tool for the characteristic protein profiles related to adhesion mucin model property was used. Three Lactobacillus plantarum strains with different rates were used proteomic analysis: L. WHE 92 (15.9%), 299 v (9.1%) and CECT 4185 (1.4%). Cell wall extracts subjected analysis differential expression using 2-DE, tryptic...
Cecropin A is a natural antimicrobial peptide that exhibits rapid, potent and long-lasting lytic activity against broad spectrum of pathogens, thus having great biotechnological potential. Here, we report system for producing bioactive cecropin in rice seeds.Transgenic plants expressing codon-optimized synthetic gene drived by an endosperm-specific promoter, either the glutelin B1 or B4 were generated. The signal sequence from N-terminally fused to coding A. We also studied whether presence...
Enterococcus faecalis WHE 96, a strain isolated from soft cheese based on its anti-Listeria activity, produced 5,494-Da bacteriocin that was purified to homogeneity by ultrafiltration and cation-exchange reversed-phase chromatographies. The amino acid sequence of this bacteriocin, named enterocin determined Edman degradation, structural gene sequenced, revealing double-glycine leader peptide. After comparison with other bacteriocins, it shown 96 new class II showed very little similarity...
ABSTRACT Enterococcus faecium IT62, isolated from ryegrass in Japan, was shown to produce three different bacteriocins, two of which had molecular masses and amino acid sequences that corresponded those enterocin L50A L50B. These peptides existed, however, as chemically modified forms were either N formylated or oxidized at Met 24 . The third bacteriocin, named IT, a mass 6,390 Da, made up 54 acids, did not correspond any known bacteriocin. However, IT identical the C-terminal part...
Cecropin A is a natural antimicrobial peptide that exhibits fast and potent activity against broad spectrum of pathogens neoplastic cells, has important biotechnological applications. However, cecropin exploitation, as for other peptides, limited by their production purification costs. Here, we report the efficient this bioactive in rice bran using oleosin 18 carrier protein. High levels were reached seeds driving expression chimeric gene strong embryo-specific own promoter, targeting to oil...
Among the mineral nutrients that are required for plant metabolism, iron (Fe) and sulphur (S) play a central role as both elements needed activity of several proteins involved in essential cellular processes. A combination physiological, biochemical molecular approaches was employed to investigate how S availability influences response Fe deficiency, using model Arabidopsis thaliana. We first observed chlorosis symptom induced by deficiency less pronounced when scarce. thus found inhibited...
SUMMARY Iron (Fe) is a micronutrient essential for plant growth and development. uptake in alkaline soil challenge most plants. In this study, we investigated the role of catechol coumarins fraxetin esculetin Fe acquisition their chelating properties. Mass spectrometry capillary electrophoresis were used to characterize Fe-coumarin complexes. To understand these complexes, genetic, molecular biochemical approaches deployed. We demonstrated that are taken up by Arabidopsis thaliana root via...
Enterococcus faecium IT62, a strain isolated from ryegrass in Japan, produces three bacteriocins (enterocins L50A, L50B, and IT) that have been previously purified the primary structures of which determined by amino acid sequencing (E. Izquierdo, A. Bednarczyk, C. Schaeffer, Y. Cai, E. Marchioni, Van Dorsselaer, S. Ennahar, Antimicrob. Agents Chemother., 52:1917-1923, 2008). Genetic analysis showed IT62 are plasmid encoded, but with structural genes specifying enterocin L50A L50B being...
ABSTRACT Root development is controlled by local and systemic regulatory mechanisms that optimize mineral nutrient uptake carbon allocation. The Autoregulation of Nodulation (AoN) pathway defines a negative regulation nodule in Legumes as way to regulate the costly production nitrogen-fixing organs. This defined response symbiotic interaction has been shown also control root formation some extent. However, it remains unclear if are under coordinated genetic regulation. Here, we identified...