A5011508481- Glutathione Transferases and Polymorphisms
- Genomics, phytochemicals, and oxidative stress
- Plant-Microbe Interactions and Immunity
- Redox biology and oxidative stress
- DNA Repair Mechanisms
- Bioenergy crop production and management
- RNA and protein synthesis mechanisms
- Nitrogen and Sulfur Effects on Brassica
- Yeasts and Rust Fungi Studies
- Photosynthetic Processes and Mechanisms
- Fungal and yeast genetics research
- RNA modifications and cancer
- Calcium Carbonate Crystallization and Inhibition
- Mycorrhizal Fungi and Plant Interactions
- Enzyme Structure and Function
- Genomics and Phylogenetic Studies
- Environmental Toxicology and Ecotoxicology
- Neurobiology and Insect Physiology Research
- Metal-Catalyzed Oxygenation Mechanisms
- Biofuel production and bioconversion
- Crustacean biology and ecology
- Plant Stress Responses and Tolerance
- Legume Nitrogen Fixing Symbiosis
- Weed Control and Herbicide Applications
- bioluminescence and chemiluminescence research
Interactions Arbres-Microorganismes
2013-2022
Université de Lorraine
2013-2022
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2022
Institut National de la Recherche Agronomique
2014
Université Sorbonne Nouvelle
2012
Sorbonne Université
2012
Université Paris-Sud
2005-2009
Centre National de la Recherche Scientifique
2002-2009
Université de Bourgogne
2002-2005
Biogéosciences
2005
Oaks are an important part of our natural and cultural heritage. Not only they ubiquitous in most common landscapes1 but have also supplied human societies with invaluable services, including food shelter, since prehistoric times2. With 450 species spread throughout Asia, Europe America3, oaks constitute a critical global renewable resource. The longevity (several hundred years) probably underlies their emblematic historical importance. Such long-lived sessile organisms must persist the face...
Summary Iron (Fe) is a major micronutrient and required for plant growth development. Nongrass species have evolved reduction‐based strategy to solubilize take up Fe. The secretion of Fe‐mobilizing coumarins (e.g. fraxetin, esculetin sideretin) by roots plays an important role in this process. Although the biochemical mechanisms leading their biosynthesis been well described, very little known about cellular subcellular localization or mobility within tissues. Spectral imaging was used...
We used the combination of preparative electrophoresis and immunological detection to isolate two new proteins from shell calcitic prisms Pinna nobilis, Mediterranean fan mussel. The amino acid composition these was determined. Both are soluble, intracrystalline, acidic. 38-kDa protein is glycosylated; 17-kDa one not. Ala, Asx, Thr, Pro represent dominant residues protein, named calprismin. An N-terminal sequence obtained This sequence, which comprises a pattern 4 cysteine residues, not...
N6-threonylcarbamoyladenosine (t6A) is a modified nucleotide found in all transfer RNAs (tRNAs) decoding codons starting with adenosine. Its role to facilitate codon–anticodon pairing and prevent frameshifting during protein synthesis. Genetic studies demonstrated that two universal proteins, Kae1/YgjD Sua5/YrdC, are necessary for t6A synthesis Saccharomyces cerevisiae Escherichia coli. In Archaea Eukarya, Kae1 part of conserved complex named kinase, endopeptidase other proteins small size...
N 6 -threonylcarbamoyladenosine (t A) is a universal tRNA modification essential for normal cell growth and accurate translation. In Archaea Eukarya, the protein Sua5 conserved KEOPS/EKC complex together catalyze t A biosynthesis. The composed of Kae1, metalloprotein belonging to ASHKA superfamily ATPases; Bud32, an atypical kinase two small proteins, Cgi121 Pcc1. this study, we investigated requirement functional role subunits biosynthesis A. We demonstrated that Pcc1, Kae1 Bud32 form...
Significance We demonstrate that, although the two phosphatases of Calvin–Benson cycle photosynthesis [sedoheptulose-1,7-bisphosphatase (SBPase) and fructose-1,6-bisphosphatase (FBPase)] share extensive structural homology, their redox-regulatory disulfides are incorporated in strikingly different positions, agreement with an independent evolutionary origin each enzyme. This article compares detail structures enzymes together regulatory catalytic properties as well phylogenies....
Poplars are extensively cultivated worldwide, and their susceptibility to the leaf rust fungus Melampsora larici-populina leads considerable damages in plantations. Despite a good knowledge of poplar life cycle, particularly epidemics on poplar, perennial status plant host obligate biotrophic lifestyle bottlenecks for molecular investigations. Following completion both M. Populus trichocarpa genome sequences, gene families involved resistance or virulence were investigated, allowing...
Summary The ectomycorrhizal symbiosis is a predominant tree–microbe interaction in forest ecosystems sustaining tree growth and health. Its establishment functioning implies long‐term intimate relationship between the soil‐borne fungi roots of trees. Mycorrhiza‐induced Small‐Secreted Proteins (MiSSPs) are hypothesized as keystone symbiotic proteins, required to set up by modifying host metabolism and/or building interfaces. L . bicolor MiSSP8 third most highly induced MiSSPs tissues it also...
The Kae1 (Kinase-associated endopeptidase 1) protein is a member of the recently identified transcription complex EKC and telomeres maintenance KEOPS in yeast. homologues are encoded by all sequenced genomes three domains life. Although annotated as putative endopeptidases, actual functions these universal proteins unknown. Here we show that purified (Pa-Kae1) from Pyrococcus abyssi an iron-protein with novel type ATP-binding site. Surprisingly, this did not exhibit activity vitro but binds...
Yeast Qri7 and human OSGEPL are members of the orthologous Kae1(OSGEP)/YgjD protein family, last class universally conserved proteins without assigned function. Phylogenetic analyses indicate that eukaryotic Qri7(OSGEPL) originated from bacterial YgjD proteins. We have recently shown archaeal Kae1 is a DNA-binding exhibits apurinic endonuclease activity in vitro . show here Qri7/OSGEPL localize mitochondria involved mitochondrial genome maintenance two model organisms, Saccharomyces...
GSTs represent a superfamily of multifunctional proteins which play crucial roles in detoxification processes and secondary metabolism. Instead promoting the conjugation glutathione to acceptor molecules as do most GSTs, members Lambda class (GSTLs) catalyse deglutathionylation reactions via catalytic cysteine residue. Three GSTL genes (Pt-GSTL1, Pt-GSTL2 Pt-GSTL3) are present Populus trichocarpa, but two transcripts, differing their 5′ extremities, were identified for Pt-GSTL3. Transcripts...
Mechanisms required for broad-spectrum or specific host colonization of plant parasites are poorly understood. As a perfect illustration, heteroecious rust fungi require two alternate plants to complete their life cycles. Melampsora larici-populina infects taxonomically unrelated plants, larch, on which sexual reproduction is achieved, and poplar, clonal multiplication occurs, leading severe epidemics in plantations. We applied deep RNA sequencing three key developmental stages M. infection...
Orchestia cavimana is a terrestrial crustacean, which cyclically stores calcium in diverticula of the midgut, form calcified amorphous concretions. These concretions are associated with proteinaceous matrix, main constituent soluble matrix Orchestin, an acidic calcium-binding protein [Testenière et al., Biochem. J. 361 (2002) 327-335]. In present paper, we clearly demonstrate that Orchestin phosphorylated on serine and tyrosine residues, but binding only occurs via phosphoserine residues. To...
Glutathione transferases (GSTs) constitute a superfamily of enzymes with essential roles in cellular detoxification and secondary metabolism plants as other organisms. Several plant GSTs, including those the Phi class (GSTFs), require conserved catalytic serine residue to perform glutathione (GSH)-conjugation reactions. Genomic analyses revealed that terrestrial have around 10 GSTFs, 8 Populus trichocarpa genome, but their physiological functions substrates are mostly unknown. Transcript...
Threonylcarbamoyladenosine (t(6)A) is a universal modification located in the anticodon stem-loop of tRNAs. In yeast, both cytoplasmic and mitochondrial tRNAs are modified. The t(6)A synthesis pathway was elucidated requires Sua5p, Kae1p, four other KEOPS complex proteins. Recent vitro work suggested that machinery Saccharomyces cerevisiae composed only two proteins, Sua5p Qri7p, member Kae1p/TsaD family (L. C. K. Wan et al., Nucleic Acids Res. 41:6332-6346, 2013,...
In most organisms, methylglyoxal (MG), a toxic metabolite by-product that causes diabetes in humans, is predominantly detoxified by the glyoxalase enzymes. This process begins with so-called “spontaneous” conjugation of MG cytoprotectant glutathione (GSH). this study, we unravel logical, but as yet unsuspected, link between detoxification and (prokaryotic) representative ubiquitous transferase (GST) We show GST model cyanobacterium plays prominent role catalyzing its GSH. finding important...
The similarities between essential molecular mechanisms in Archaea and Eukarya make it possible to discover, using comparative genomics, new fundamental conserved these two domains. We are studying a complex of proteins whose precise biological role biochemical function remain unknown. One them is universal protein known as Kae1 (kinase-asociated endopeptidase 1). second serine/threonine kinase corresponding the Bud32 Saccharomyces cerevisiae PRPK (p53-related kinase) humans. genes encoding...