A5059931217- Plant-Microbe Interactions and Immunity
- Wheat and Barley Genetics and Pathology
- Plant Virus Research Studies
- Genetics and Plant Breeding
- Transgenic Plants and Applications
- Mycorrhizal Fungi and Plant Interactions
- Genetic Mapping and Diversity in Plants and Animals
- Plant pathogens and resistance mechanisms
- Toxin Mechanisms and Immunotoxins
- Plant tissue culture and regeneration
- Plant Pathogenic Bacteria Studies
- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Yeasts and Rust Fungi Studies
- Ancient and Medieval Archaeology Studies
- Historical and Archaeological Studies
- Image Processing and 3D Reconstruction
- Plant Parasitism and Resistance
- Agronomic Practices and Intercropping Systems
- Genetic diversity and population structure
- Mobile Crowdsensing and Crowdsourcing
- Botanical Research and Chemistry
- Fungal Biology and Applications
- Plant Gene Expression Analysis
- Genomics and Phylogenetic Studies
Norwich Research Park
2018-2025
John Innes Centre
2018-2025
Sainsbury Laboratory
2021-2024
University of East Anglia
2021-2024
Université de Lorraine
2015-2018
Interactions Arbres-Microorganismes
2017-2018
Laboratoire Interdisciplinaire des Environnements Continentaux
2017
Artistic Realization Technologies
2015
Oaks are an important part of our natural and cultural heritage. Not only they ubiquitous in most common landscapes1 but have also supplied human societies with invaluable services, including food shelter, since prehistoric times2. With 450 species spread throughout Asia, Europe America3, oaks constitute a critical global renewable resource. The longevity (several hundred years) probably underlies their emblematic historical importance. Such long-lived sessile organisms must persist the face...
Plant pathogens cause recurrent epidemics, threatening crop yield and global food security. Efforts to retool the plant immune system have been limited modifying natural components can be nullified by emergence of new pathogen strains. Made-to-order synthetic receptors provide an opportunity tailor resistance genotypes present in field. In this work, we show that nucleotide-binding, leucine-rich repeat (NLRs) used as scaffolds for nanobody (single-domain antibody fragment) fusions bind...
Understanding the function of genes within staple crops will accelerate crop improvement by allowing targeted breeding approaches. Despite their importance, a lack genomic information and resources has hindered functional characterisation in major crops. The recent release high-quality reference sequences for these underpins suite genetic that support basic research breeding. For wheat, include gene model annotations, expression atlases networks provide about putative function. Sequenced...
Nucleotide-binding and leucine-rich repeat receptors (NLRs) are intracellular plant immune that recognize pathogen effectors secreted into the cell. Canonical NLRs typically contain three conserved domains including a central nucleotide binding (NB-ARC) domain, C-terminal repeats (LRRs) an N-terminal domain. A subfamily of additional noncanonical domain(s) have potentially evolved from integration effector targets in canonical NLR structure. These with extra thus referred to as integrated...
Summary Nucleotide‐binding domain and leucine‐rich repeat (NLR) proteins with pathogen sensor activities have evolved to initiate immune signaling by activating helper NLRs. However, the mechanisms underpinning NLR activation NLRs remain poorly understood. Although coiled coil (CC) type such as Potato virus X disease resistance protein Rx been shown activate oligomerization of their downstream helpers NRC2, NRC3 NRC4, domains involved in sensor–helper are not known. Here, we used...
Abstract Nutrient acquisition is crucial for sustaining life. Plants develop beneficial intracellular partnerships with arbuscular mycorrhiza (AM) and nitrogen-fixing bacteria to surmount the scarcity of soil nutrients tap into atmospheric dinitrogen, respectively 1,2 . Initiation these root endosymbioses requires symbiont-induced oscillations in nuclear calcium (Ca 2+ ) concentrations cells 3 How nuclear-localized ion channels, cyclic nucleotide-gated channel (CNGC) 15 DOESN’T MAKE...
Mechanisms required for broad-spectrum or specific host colonization of plant parasites are poorly understood. As a perfect illustration, heteroecious rust fungi require two alternate plants to complete their life cycles. Melampsora larici-populina infects taxonomically unrelated plants, larch, on which sexual reproduction is achieved, and poplar, clonal multiplication occurs, leading severe epidemics in plantations. We applied deep RNA sequencing three key developmental stages M. infection...
ABSTRACT Plant pathogens cause recurrent epidemics that threaten crop yield and global food security. Efforts to retool the plant immune system have been limited modifying natural components can be nullified by emergence of new pathogen races. Therefore, there is a need develop made-to-order synthetic receptors with resistance tailored genotypes present in field. Here we show used as scaffolds for VHH nanobody fusions bind fluorescent proteins (FPs). The receptor-nanobody signal presence...
SignificanceOscillations in intracellular calcium concentration play an essential role the regulation of multiple cellular processes. In plants capable root endosymbiosis with nitrogen-fixing bacteria and/or arbuscular mycorrhizal fungi, nuclear localized oscillations are to transduce microbial signal. Although ion channels required generate have been identified, their mechanisms unknown. Here, we combined proteomics and engineering approaches demonstrate that calcium-bound form calmodulin 2...
Abstract Since 2016, hobby metal-detecting is legal in Flanders (Belgium), although it was unofficially tolerated for many years before. However, research on metal-detected artefacts hindered by a low reporting rate. The MEDEA project aims to address this encouraging detectorists record their finds an online platform. Finds experts are invited enrich records with further information and thus instigate rewarding feedback cycle. This paper discusses MEDEA’s ‘Human-Centred Design’ development...
Nucleotide-binding leucine-rich-repeat (LRR) receptors (NLRs) with non-canonical integrated domains (NLR-IDs) are widespread in plant genomes. Zinc-finger BED (named after the Drosophila proteins Boundary Element-Associated Factor and DNA Replication-related Element binding Factor, named hereafter) among most frequently found IDs. Five BED-NLRs conferring resistance against bacterial fungal pathogens have been characterized. However, it is unknown whether function a manner similar to other...
Crop diseases reduce wheat yields by ~25% globally and thus pose a major threat to global food security 1 .Genetic resistance can crop losses in the field be selected for through use of molecular markers.However, genetic often breaks down following changes pathogen virulence, as experienced with yellow (stripe) rust fungus Puccinia striiformis f. sp.tritici (Pst) 2 .This highlights need (i) identify genes that alone or combination provide broad-spectrum (ii) increase our understanding...
To adapt to the challenges of climate change and growing world population, it is vital increase global crop production. Understanding function genes within staple crops will accelerate improvement by allowing targeted breeding approaches. Despite importance wheat, which provides 20 % calories consumed humankind, a lack genomic information resources has hindered functional characterisation in this species. The recent release high-quality reference sequence for wheat underpins suite genetic...
Research in Arabidopsis and other model species has uncovered mechanisms regulating important biological processes plants. With the advent of high quality functional genomic resources wheat it is now possible to use this knowledge for crop improvement directly wheat. In current review, we describe some recent developments genomics focussing on published publicly available tools. We lay out a roadmap how make them include case study exemplify them. hope review will be helpful guide plant...
To adapt to the challenges of climate change and growing world population, it is vital increase global crop production. Understanding function genes within staple crops will accelerate improvement by allowing targeted breeding approaches. Despite importance wheat, which provides 20 % calories consumed humankind, a lack genomic information resources has hindered functional characterisation in this species. The recent release high-quality reference sequence for wheat underpins suite genetic...
Summary Mechanims required for broad spectrum or specific host colonization of plant parasites are poorly understood. As a perfect illustration, heteroecious rust fungi require two alternate plants to complete their life cycle. Melampsora larici-populina infects taxonomically unrelated plants, larch on which sexual reproduction is achieved and poplar clonal multiplication occurs leading severe epidemics in plantations. High-depth RNA sequencing was applied three key developmental stages M....