A5002193996- Wheat and Barley Genetics and Pathology
- Genetic Mapping and Diversity in Plants and Animals
- Genetics and Plant Breeding
- Crop Yield and Soil Fertility
- Food composition and properties
- Genetic and Environmental Crop Studies
- Gene expression and cancer classification
- Genomic variations and chromosomal abnormalities
- Plant Disease Resistance and Genetics
- Plant nutrient uptake and metabolism
- Chromosomal and Genetic Variations
- Agricultural pest management studies
- Acute Myeloid Leukemia Research
- Biofuel production and bioconversion
- Plant Micronutrient Interactions and Effects
- Plant-Microbe Interactions and Immunity
- Epigenetics and DNA Methylation
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Genetic diversity and population structure
- Microtubule and mitosis dynamics
- Mycotoxins in Agriculture and Food
- Cancer-related gene regulation
- Fungal and yeast genetics research
- Plant Pathogens and Fungal Diseases
Norwich Research Park
2015-2025
John Innes Centre
2016-2025
Rothamsted Research
2024
Chinese Academy of Agricultural Sciences
2024
University of Bristol
2024
Max Planck Society
2001-2012
Medizinische Hochschule Hannover
2004-2009
Sidney Kimmel Comprehensive Cancer Center
2004
Institute of Molecular and Cell Biology
2004
Seedling root traits of wheat (Triticum aestivum L.) have been shown to be important for efficient establishment and linked mature plant such as height yield. A phenotyping pipeline, consisting a germination paper-based screen combined with image segmentation analysis software, was developed used characterize seedling in 94 doubled haploid progeny derived from cross between the winter cultivars Rialto Savannah. Field experiments were conducted measure height, grain yield, nitrogen (N) uptake...
Abstract Harnessing genetic diversity in major staple crops through the development of new breeding capabilities is essential to ensure food security 1 . Here we examined and phenotypic A. E. Watkins landrace collection 2 bread wheat ( Triticum aestivum ), a global cereal, by whole-genome re-sequencing 827 landraces 208 modern cultivars in-depth field evaluation spanning decade. We found that are derived from two seven ancestral groups maintain very long-range haplotype integrity. The...
A high level of genetic diversity was found in the A. E. Watkins bread wheat landrace collection. Genotypic information used to determine population structure and develop germplasm resources. In 1930s acquired cultivars (Triticum aestivum L.) from official channels board Trade London, many which originated local markets 32 countries. The geographic distribution 826 current collection, here called covers Asian European countries some Africa. were genotyped with 41 microsatellite markers order...
Grain weight (GW) and number per unit area of land (GN) are the primary components grain yield in wheat. In segregating populations both often show a negative correlation among themselves. Here we use recombinant doubled haploid population 105 individuals developed from CIMMYT varieties Weebill Bacanora to understand relative contribution these their interaction with each other. was chosen for its high GW GN. The phenotyped Mexico, Argentina, Chile UK. Two loci influencing were indicated on...
Understanding the function of genes within staple crops will accelerate crop improvement by allowing targeted breeding approaches. Despite their importance, a lack genomic information and resources has hindered functional characterisation in major crops. The recent release high-quality reference sequences for these underpins suite genetic that support basic research breeding. For wheat, include gene model annotations, expression atlases networks provide about putative function. Sequenced...
Abstract Crop productivity must increase at unprecedented rates to meet the needs of growing worldwide population. Exploiting natural variation for genetic improvement crops plays a central role in increasing productivity. Although current genomic technologies can be used high-throughput identification variation, methods efficiently exploiting this potential targeted, systematic manner are lacking. Here, we developed haplotype-based approach identify diversity crop using genome assemblies...
Understanding the genomic complexity of bread wheat (Triticum aestivum L.) is a cornerstone in quest to unravel processes domestication and following adaptation domesticated wide variety environments across globe. Additionally, it importance for future improvement crop, particularly light climate change. Focusing on after domestication, nested association mapping (NAM) panel 60 segregating biparental populations was developed, mainly involving landrace accessions from core set Watkins...
Sequence exchange between homologous chromosomes through crossing over and gene conversion is highly conserved among eukaryotes, contributing to genome stability genetic diversity. A lack of recombination limits breeding efforts in crops; therefore, increasing rates can reduce linkage drag generate new combinations.We use computational analysis 13 recombinant inbred mapping populations assess crossover frequency the hexaploid wheat (Triticum aestivum). We observe that high-frequency sites...
Abstract Since emerging in Brazil 1985, wheat blast has spread throughout South America and recently appeared Bangladesh Zambia. Here we show that two resistance genes, Rwt3 Rwt4 , acting as host-specificity barriers against non- Triticum pathotypes encode a nucleotide-binding leucine-rich repeat immune receptor tandem kinase, respectively. Molecular isolation of these genes will enable study the molecular interaction between pathogen effector host genes.
Earliness per se (Eps) genes account for the variation in flowering time when vernalization and photoperiod requirements are satisfied. Genomics bioinformatics approaches were used to describe allelic 40 Triticum aestivum predicted, by synteny with Brachypodium distachyon, be 1DL Eps region. Re-sequencing revealed that varieties carrying early heading alleles at this locus, Spark Cadenza, carry a subtelomeric deletion including several genes. The equivalent region Rialto Avalon is intact. A...
Abstract Background In wheat, nitrogen (N) remobilization from vegetative tissues to developing grains largely depends on genetic and environmental factors. The evaluation of potential crops under limited resource inputs such as N supply would provide an opportunity identify N-efficient lines with improved utilisation efficiency yield potential. We assessed the variation in wheat recombinant inbred (RILs) for uptake, partitioning, towards grain, its association grain protein concentration...
Abstract Wheat blast, caused by the fungus Magnaporthe oryzae , threatens global cereal production since its emergence in Brazil 1985 and recently spread to Bangladesh Zambia. Here we demonstrate that AVR-Rmg8 effector, common wheat-infecting isolates, is recognized gene Pm4 previously shown confer resistance specific races of Blumeria graminis f. sp. tritici cause powdery mildew wheat. We show alleles differ their recognition different alleles, some only seedling leaves but not spikes,...
To gain more insight into the role of chromosomal instability (CIN), cytogenetic hallmark most solid tumors, we performed fluorescence in situ hybridization (FISH) on interphase nuclei cytological specimens enabling correct detection chromosome copies intact tumor cells 18 well (G1), moderately (G2), or poorly (G3) differentiated hepatocellular carcinomas (HCCs). A close correlation between morphological dedifferentiation and increasing copy numbers variation FISH signals was seen for...
Vernalization, photoperiod and the relatively poorly defined earliness per se (eps) genes regulate flowering in plants. We report here validation of a major eps quantitative trait locus (QTL) located on wheat 1DL using near isogenic lines (NILs). used four independent pairs NILs derived from cross between Spark Rialto winter varieties, grown both field controlled environments. carrying allele, by QTL flanking markers Xgdm111 Xbarc62, consistently flowered 3-5 days earlier when fully...
The importance of wheat as a food crop makes it major target for agricultural improvements. As one the most widely grown cereal grains, together with maize and rice, is leading provider calories in global diet, constituting 29% production 2015. In last few decades, however, yields have plateaued, suggesting that green revolution, at least wheat, might run its course new sources genetic variation are urgently required. overall aim our work was to identify novel may then be used enable...
Abstract Septoria tritici blotch (STB), caused by the Dothideomycete fungus Zymoseptoria , is one of most damaging diseases bread wheat ( Triticum aestivum ) 1 and target costly fungicide applications 2 . In line with fungus’s apoplastic lifestyle, STB resistance genes isolated to date encode receptor-like kinases (RLKs) including a wall-associated kinase Stb6 cysteine-rich Stb16q 3,4 Here we used genome-wide association studies on diverse panel 300 whole-genome shotgun-sequenced landraces...
Abstract Background: Gene silencing through aberrant CpG island methylation is the most extensively analyzed epigenetic event in human tumorigenesis and has huge diagnostic prognostic potential. Methylation patterns are often very heterogeneous, however, presenting a serious challenge for development of assays purposes. Methods: We used Pyrosequencing™ technology to determine status 68 sites CDKN2B gene [cyclin-dependent kinase inhibitor 2B (p15, inhibits CDK4)], frequently hypermethylated...
Pod corn is a classic morphological mutant of maize in which the mature kernels cob are covered by glumes, contrast to generally grown varieties naked. corn, known since pre-Columbian times, result dominant gain-of-function mutation at Tunicate ( Tu ) locus. Some articles 20th century genetics reported that locus complex, but molecular details remained elusive. Here, we show pod caused cis -regulatory and duplication ZMM19 MADS-box gene. Although WT contains single-copy gene expressed...
Abstract Genetic studies increasingly rely on high-throughput phenotyping, but the resulting longitudinal data pose analytical challenges. We used canopy height from an automated field phenotyping platform to compare several approaches scanning for quantitative trait loci (QTLs) and performing genomic prediction in a wheat recombinant inbred line mapping population based up 26 sampled time points (TPs). detected four persistent QTLs (i.e. expressed most of growing season), with both...