A5043176926- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Light effects on plants
- Plant Reproductive Biology
- Plant-Microbe Interactions and Immunity
- Wheat and Barley Genetics and Pathology
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Plant Stress Responses and Tolerance
- Plant Disease Resistance and Genetics
- High Altitude and Hypoxia
- Cryospheric studies and observations
- Genetics, Aging, and Longevity in Model Organisms
- Circadian rhythm and melatonin
- Sulfur-Based Synthesis Techniques
- Plant Genetic and Mutation Studies
- Heat shock proteins research
- Chemical Synthesis and Reactions
- Fungal and yeast genetics research
- Plant Parasitism and Resistance
- Plant and animal studies
- Plant and Biological Electrophysiology Studies
- Greenhouse Technology and Climate Control
University of Glasgow
2022-2024
Norwich Research Park
2017-2023
John Innes Centre
2017-2023
Plants integrate widely fluctuating temperatures to monitor seasonal progression. Here, we investigate the temperature signals in field conditions that result vernalisation, mechanism by which flowering is aligned with spring. We find multiple, distinct aspects of profile contribute vernalisation. In autumn, transient cold promote transcriptional shutdown Arabidopsis FLOWERING LOCUS C (FLC), independently factors conferring epigenetic memory. As winter continues, expression VERNALIZATION...
Abstract Since emerging in Brazil 1985, wheat blast has spread throughout South America and recently appeared Bangladesh Zambia. Here we show that two resistance genes, Rwt3 Rwt4 , acting as host-specificity barriers against non- Triticum pathotypes encode a nucleotide-binding leucine-rich repeat immune receptor tandem kinase, respectively. Molecular isolation of these genes will enable study the molecular interaction between pathogen effector host genes.
Plants monitor many aspects of their fluctuating environments to help align development with seasons. Molecular understanding how noisy temperature cues are registered has emerged from dissection vernalization in Arabidopsis, which involves a multiphase cold-dependent silencing the floral repressor locus FLOWERING LOCUS C (FLC). Cold-induced transcriptional precedes low probability PRC2 epigenetic switching mechanism. The switch requires absence warm temperatures as well long-term cold...
Many organisms need to respond complex, noisy environmental signals for developmental decision making. Here, we dissect how Arabidopsis plants integrate widely fluctuating field temperatures over month-long timescales progressively upregulate VERNALIZATION INSENSITIVE3 (VIN3) and silence FLOWERING LOCUS C (FLC), aligning flowering with spring. We develop a mathematical model vernalization that operates on multiple timescales—long term (month), short (day), current (hour)—and is constrained...
Speciation through the emergence of reproductive isolation barriers is a fundamental concept evolutionary biology. Here we use two closely related but phenotypically distinct Gymnadenia orchid species and their proposed hybrids to study associated with this phenomenon. We analysed potential pre- post-zygotic hybridisation conducted first comprehensive analysis pollinator-influenced phenotypic traits in both parents hybrids. also uncover previously uncharacterised, much hypothesised, genetic...
In Arabidopsis thaliana , the cold-induced epigenetic regulation of FLOWERING LOCUS C ( FLC ) involves distinct phases Polycomb repressive complex 2 (PRC2) silencing. During cold, a PHD–PRC2 metastably and digitally nucleates H3K27me3 within . On return to warm, spreads across locus delivering maintain long-term Here, we studied natural variation in this process accessions, exploring Lov-1, which shows reactivation on feature characteristic perennial Brassicaceae This analysis identifies an...
In Arabidopsis thaliana, winter is registered during vernalization through the temperature-dependent repression and epigenetic silencing of floral repressor FLOWERING LOCUS C (FLC) . Natural accessions show considerable variation in vernalization. However, which aspect FLC mechanism most important for adaptation to different environments unclear. By analysing dynamics natural variants mutants throughout three field sites, we find that autumnal expression, rather than silencing, major...
Abstract Most rust resistance genes thus far isolated from wheat have a very limited number of functional alleles. Here, we report the isolation most alleles at stem gene locus SR9 . The seven previously reported ( Sr9a , Sr9b Sr9d Sr9e Sr9f Sr9g and Sr9h ) are characterised using synergistic strategy. Loss-of-function mutants and/or transgenic complementation used to confirm two haplotypes Sr9e_h1 Sr9e_h2 ), Each allele encodes highly related nucleotide-binding site leucine-rich repeat...
Quantitative gene regulation at the cell population level can be achieved by two fundamentally different modes of individual copies. A ‘digital’ mode involves binary ON/OFF expression states, with population-level variation arising from proportion copies in each state, while an ‘analog’ graded levels copy. At Arabidopsis floral repressor FLOWERING LOCUS C (FLC), Polycomb silencing is known to facilitate quantitative epigenetic memory response cold. However, whether FLC before cold analog or...
Abstract Plants exploit developmental plasticity to adapt their growth and development prevailing environmental cues. This provides a selective competitive advantage in nature but is obstructive during large-scale, intensive agricultural practices since economically important traits (including vegetative flowering time) can widely vary depending on local conditions. prevents accurate prediction of harvesting times produces variable crop. We sought restrict by manipulating signalling systems...
Abstract Quantitative gene regulation at the cell population-level can be achieved by two fundamentally different modes of individual copies. A “digital” mode involves binary ON/OFF expression states, with variation arising from proportion copies in each state, while an “analog” graded levels copy. At Arabidopsis floral repressor FLOWERING LOCUS C (FLC), Polycomb silencing is known to facilitate quantitative epigenetic memory response cold. However, whether FLC before cold analog or digital...
Abstract Most resistance genes thus far isolated from wheat have a very limited number of functional alleles, with the exception powdery mildew PM3 locus. Here we report isolation most alleles at stem rust gene locus SR9 , representing largest multi-allelic in common wheat. The seven previously reported ( Sr9a Sr9b Sr9d Sr9e Sr9f Sr9g and Sr9h ) were characterised using synergistic strategy. Loss-of-function mutants and/or transgenic complementation used to confirm two haplotypes Sr9e_h1...
Abstract Plants monitor temperatures over long timescales to assess seasons and time developmental transitions. In Arabidopsis thaliana , winter is registered during vernalization through the temperature-dependent repression epigenetic silencing of floral repressor FLOWERING LOCUS C (FLC) . Natural accessions show considerable variation in vernalization, however which aspect FLC mechanism most important for adaptation different climates not clear. By analyzing natural variants throughout...