A5014357005- Mycorrhizal Fungi and Plant Interactions
- Fungal Biology and Applications
- Forest Ecology and Biodiversity Studies
- Trace Elements in Health
- Plant Stress Responses and Tolerance
- Heavy metals in environment
- Legume Nitrogen Fixing Symbiosis
- Aluminum toxicity and tolerance in plants and animals
- Plant-Microbe Interactions and Immunity
- Ecology and Vegetation Dynamics Studies
- Identification and Quantification in Food
- Genetic diversity and population structure
- Molecular Biology Techniques and Applications
- Plant and fungal interactions
- Fungal and yeast genetics research
- Plant nutrient uptake and metabolism
- Environmental DNA in Biodiversity Studies
- Plant Micronutrient Interactions and Effects
- Chromium effects and bioremediation
- Plant Genetic and Mutation Studies
- Plant and Biological Electrophysiology Studies
- Evolution and Genetic Dynamics
- Yeasts and Rust Fungi Studies
- Plant responses to water stress
- Agricultural Science and Fertilization
Vrije Universiteit Brussel
2007-2024
Interactions Arbres-Microorganismes
2016-2021
Université de Lorraine
2016-2021
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2021
Hasselt University
2007-2019
ENVIRON (United States)
2017
Francis Martin and colleagues report genome sequences for 18 species of mycorrhizal fungi a phylogenomic analysis including 32 other fungal genomes. The study identifies cell wall-degradation genes lost in all true ectomycorrhizal and, using gene expression data, finds candidate the establishment symbiosis. To elucidate genetic bases lifestyle evolution, we sequenced new genomes, 13 (ECM), orchid (ORM) ericoid (ERM) species, five saprotrophs, which analyzed along with Ectomycorrhizal have...
Summary Research on mycorrhizal symbiosis has been slowed by a lack of established study systems. To address this challenge, we have developing Suillus , widespread ecologically and economically relevant fungal genus primarily associated with the plant family Pinaceae, into model system for studying ectomycorrhizal (ECM) associations. Over last decade, compiled extensive genomic resources, culture libraries, phenotype database, protocols manipulating fungi without their tree partners. Our...
Summary While there has been significant progress characterizing the ‘symbiotic toolkit’ of ectomycorrhizal (ECM) fungi, how host specificity may be encoded into ECM fungal genomes remains poorly understood. We conducted a comparative genomic analysis specialists and generalists, focusing on specialist genus Suillus . Global analyses genome dynamics across 46 species were assessed, along with targeted three classes molecules previously identified as important determinants specificity: small...
Summary The ectomycorrhizal symbiosis is a predominant tree–microbe interaction in forest ecosystems sustaining tree growth and health. Its establishment functioning implies long‐term intimate relationship between the soil‐borne fungi roots of trees. Mycorrhiza‐induced Small‐Secreted Proteins (MiSSPs) are hypothesized as keystone symbiotic proteins, required to set up by modifying host metabolism and/or building interfaces. L . bicolor MiSSP8 third most highly induced MiSSPs tissues it also...
Abstract Human‐altered environments can shape the evolution of organisms. Fungi are no exception, although little is known about how they withstand anthropogenic pollution. Here, we document adaptation in mycorrhizal fungus Suillus luteus driven by soil heavy metal contamination. Genome scans across individuals from recently polluted and nearby unpolluted soils Belgium revealed low divergence isolates evidence population structure based on type. However, detected single nucleotide...
In nature, plants are simultaneously exposed to different abiotic (e.g., heat, drought, and salinity) biotic bacteria, fungi, insects) stresses. Climate change anthropogenic pressure expected intensify the frequency of stress factors. Although well equipped with unique common defense systems protecting against stressors, they may compromise their growth development for survival in such challenging environments. Ionizing radiation is a peculiar factor capable causing clustered damage....
Abstract The dung microbiome is a complex system that highly influenced by species and diet. This study characterized the bacterial fungal communities of five herbivore inhabiting National Park Zuid‐Kennemerland, Netherlands. selected were rabbit ( Oryctolagus cuniculus L.), cow Bos taurus horse Equus ferus caballus fallow deer Dama dama European bison Bison bonasus L.). We explored effects distinct digestive physiology (ruminants vs. non‐ruminants) diverse dietary preferences on microbial...
On Zn-polluted soils, populations of the ectomycorrhizal basidiomycete Suillus bovinus exhibit an elevated Zn tolerance when compared to on non-polluted sites. To elucidate mechanism tolerance, time-course uptake was studied in isolates with contrasting tolerance. Unidirectional fluxes and subcellular compartmentation were investigated through radiotracer flux analyses. Fluorescence imaging used support compartmentation. After 2 h exposure 200 μM Zn, significantly more accumulated...
Abstract Zinc (Zn) is a major soil contaminant and high Zn levels can disrupt growth, survival, reproduction of fungi. Some fungal species evolved tolerance through cell processes mitigating toxicity, although the genes detailed mechanisms underlying mycorrhizal remain unexplored. To fill this gap in knowledge, we investigated gene expression ectomycorrhizal fungus Suillus luteus. We found that mainly constitutive trait also be environmentally dependent. S. luteus associated with differences...
Summary Two genes, SlZnT1 and SlZnT2 , encoding Cation Diffusion Facilitator (CDF) family transporters were isolated from Suillus luteus mycelium by genome walking. Both gene models are very similar phylogenetic analysis indicates that they most likely the result of a recent duplication event. Comparative sequence deduced proteins predicts them to be Zn transporters. This function was confirmed functional in yeast for . able restore growth highly sensitive mutant Δzrc1 localized vacuolar...
Trees are able to colonize, establish and survive in a wide range of soils through associations with ectomycorrhizal (EcM) fungi. Proper functioning EcM fungi implies the differentiation structures within fungal colony. A symbiotic structure is dedicated nutrient exchange extramatricular mycelium explores soil for nutrients. Eventually, basidiocarps develop assure last stages sexual reproduction. The aim this study understand how an fungus uses its gene set support functional development...
The basidiomycete Suillus luteus is an important member of the ectomycorrhizal community that thrives in heavy metal polluted soils covered with pioneer pine forests. This study aimed to identify potential chelators S. luteus. Two metallothionein (MT) coding genes, SlMTa and SlMTb, were identified. When heterologously expressed yeast, both SlMTb can rescue Cu sensitive mutant from toxicity. In luteus, transcription induced by but not Cd or Zn. Several putative Cu-sensing metal-response...
Zn is an essential micronutrient but may become toxic when present in excess. In Zn-contaminated environments, trees can be protected from toxicity by their root-associated micro-organisms, particular ectomycorrhizal fungi. The mechanisms of cellular homeostasis fungi and contribution to the host tree's status are however not yet fully understood. aim this study was identify characterize transporters involved uptake fungus S. luteus, a cosmopolitan pine mycobiont. known predominantly...
Ectomycorrhizal (ECM) fungi are important root symbionts of trees, as they can have significant effects on the nutrient status plants. In polluted environments, particular ECM protect their host tree from Zn toxicity by restricting transfer while securing supply essential nutrients. However, mechanisms and regulation cellular homeostasis in largely unknown, it remains unclear how affect This study focuses characterization a ZIP (Zrt/IrtT-like protein) transporter, SlZRT2, fungus Suillus...
Ectomycorrhizal (ECM) fungi develop a mutualistic symbiotic interaction with the roots of their host plants. During this process, they undergo series developmental transitions from running hyphae in rhizosphere to coenocytic forming finger-like structures within root apoplastic space. These transitions, which involve profound, symbiosis-associated metabolic changes, also entail substantial transcriptome reprogramming coordinated waves differentially expressed genes. To date, little is known...