A5023362799- Advanced Fluorescence Microscopy Techniques
- Spectroscopy Techniques in Biomedical and Chemical Research
- Lipid Membrane Structure and Behavior
- Luminescence and Fluorescent Materials
- Neuroscience and Neuropharmacology Research
- Photoacoustic and Ultrasonic Imaging
- RNA Interference and Gene Delivery
- Mycorrhizal Fungi and Plant Interactions
- Molecular Sensors and Ion Detection
- MicroRNA in disease regulation
- Field-Flow Fractionation Techniques
- Cardiomyopathy and Myosin Studies
- Extracellular vesicles in disease
- Cell Image Analysis Techniques
- Cardiovascular Effects of Exercise
- Spectroscopy and Quantum Chemical Studies
- Cardiac electrophysiology and arrhythmias
- Plasmonic and Surface Plasmon Research
- Advanced biosensing and bioanalysis techniques
- Nanoparticles: synthesis and applications
- Metamaterials and Metasurfaces Applications
- Endodontics and Root Canal Treatments
- Hematopoietic Stem Cell Transplantation
- Proteoglycans and glycosaminoglycans research
- Conducting polymers and applications
Hasselt University
2012-2022
KU Leuven
2009-2019
Agora
2018
Flemish Institute for Technological Research
2014-2017
University of Alberta
2016
Transnational University Limburg
2008-2013
Transnational University Limburg
2009-2012
We demonstrate circular dichroism (CD) in the second harmonic generation (SHG) signal from chiral assemblies of G-shaped nanostructures made gold. The arrangement G shapes is crucial since upon reordering them SHG-CD effect disappears. Microscopy reveals SHG "hotspots" assemblies, which originate enantiomerically sensitive plasmon modes, having novel property exhibiting a geometry themselves relation with handedness material. These results open new frontiers studying chirality.
Fluorescence recovery after photobleaching (FRAP) is a versatile tool for determining diffusion and interaction/binding properties in biological material sciences. An understanding of the mechanisms controlling requires deep structure-interaction-diffusion relationships. In cell biology, instance, this applies to movement proteins lipids plasma membrane, cytoplasm nucleus. industrial applications related pharmaceutics, foods, textiles, hygiene products cosmetics, solutes solvent molecules...
Abstract A visible‐light‐excitable, ratiometric, brightly fluorescent pH indicator for measurements in the range 5–7 has been designed and synthesized by conjugatively linking BODIPY fluorophore at 3‐position to pH‐sensitive ligand imidazole through an ethenyl bridge. The probe is available as cell membrane permeable methyl ester 8‐(4‐carbomethoxyphenyl)‐4,4‐difluoro‐3‐[2‐(1 H‐ imidazol‐4‐yl)ethenyl]‐1,5,7‐trimethyl‐3 a ,4 ‐diaza‐4‐bora‐ s ‐indacene ( I ) corresponding water‐soluble sodium...
The heterogeneity in composition and interaction within the cellular membrane translates into a wide range of diffusion coefficients its constituents. Therefore, several complementary microfluorimetric techniques such as fluorescence correlation spectroscopy (FCS), recovery after photobleaching (FRAP) single-particle tracking (SPT) have to be applied explore dynamics components. recently introduced raster image (RICS) offers much wider dynamic than each these methods separately allows for...
In optics, chirality is typically associated with circularly polarized light. Here we present a novel way to detect the handedness of chiral materials linearly We performed Second Harmonic Generation (SHG) microscopy on G-shaped planar nanostructures made gold. The SHG response originates in distinctive hotspots, whose arrangement dependent handedness. These results uncover new directions for studying artificial materials.
Here we describe a new BODIPY-based membrane probe (1) that provides an alternative to dialkylcarbocyanine dyes, such as DiI-C18, can be excited in the blue spectral region. Compound 1 has unbranched octadecyl chains at 3,5-positions and meso-amino function. In organic solvents, absorption emission maxima of are determined mainly by solvent acidity dipolarity. The fluorescence quantum yield is high reaches 0.93 2-propanol. decays well fitted with single-exponential pure solvents small giant...
Summary Two genes, SlZnT1 and SlZnT2 , encoding Cation Diffusion Facilitator (CDF) family transporters were isolated from Suillus luteus mycelium by genome walking. Both gene models are very similar phylogenetic analysis indicates that they most likely the result of a recent duplication event. Comparative sequence deduced proteins predicts them to be Zn transporters. This function was confirmed functional in yeast for . able restore growth highly sensitive mutant Δzrc1 localized vacuolar...
The development of a universal probe to assess the phase lipid membrane is one most ambitious goals for fluorescence spectroscopy. ability well-known molecule as Laurdan reach this aim here exploited behavior fully characterized in dipalmitoylphosphatidylcholine (DPPC) solid gel (So) by means molecular dynamics simulations. can take two conformations, depending on whether carbonyl oxygen points toward β-position naphthalene core (Conf-I) or α-position (Conf-II). We observe that Conf-I has an...
Summary Objective Febrile seizures ( FS ) are fever‐associated convulsions, being the most common seizure disorder in early childhood. A subgroup of these children later develops epilepsy characterized by a hyperexcitable neuronal network hippocampus. Hippocampal excitability is regulated hippocampal dentate gyrus DG where postnatal neurogenesis occurs. Experimental increase survival newborn granule cells DGC s), yet significance this subpopulation to remains unclear. In current study, we...
Abstract The tetrameric red fluorescent protein from Discosoma sp. coral (DsRed) has previously been engineered to produce dimeric and monomeric variants with excitation emission profiles that span the visible spectrum. brightest of effectively DsRed is tdTomato—a tandem fusion a variant. Here we describe engineering brighter (RRvT), green (GGvT), green–red heterodimeric (GRvT) tdTomato variants. GRvT exhibited 99 % intramolecular FRET efficiency, resulting in long Stokes shift fluorescence....
Fluorescent conjugated polymers formulated in nanoparticles show attractive properties to be used as bioimaging probes. However, their fluorescence brightness is generally limited by quenching phenomena due interchain aggregation the confined nanoparticle space. In this work, branched polymer networks are investigated a way enhance photoluminescence quantum yield of resulting (CPNs). 1,3,5-Tribromobenzene and 2,2′,7,7′-tetrabromo-9,9′-spirobifluorene chosen branching moieties added 3 or 5...
Ectomycorrhizal (ECM) fungi are important root symbionts of trees, as they can have significant effects on the nutrient status plants. In polluted environments, particular ECM protect their host tree from Zn toxicity by restricting transfer while securing supply essential nutrients. However, mechanisms and regulation cellular homeostasis in largely unknown, it remains unclear how affect This study focuses characterization a ZIP (Zrt/IrtT-like protein) transporter, SlZRT2, fungus Suillus...
Fluorescence recovery after photobleaching (FRAP) carried out on a confocal laser-scanning microscope (CLSM) performs well for photobleached disks that are large compared to the resolution of bleaching beam. For smaller approaching this resolution, current FRAP models providing closed-form solution do not allow one extract diffusion coefficient accurately. The new generalized disk model we present addresses shortcoming by bringing into account and total imaging resolution. A is obtained...
Alginate gels with naturally occurring macroscopic capillaries have been used as a model system to study the interplay between laminar flow and diffusion of nanometer-sized solutes in real time. Calcium alginate that contain homogeneously distributed parallel-aligned capillary structures were formed by external addition crosslinking ions an sol. The effects different rates (0, 1, 10, 50 100 μl min−1) three probes (fluorescein, 10 kDa 500 fluorescein isothiocyanate-dextran) on across wall...
Fibroblast activation protein-α (FAPα) is a membrane protein with dipeptidyl-peptidase and type I collagenase activity expressed during fetal growth. At the age of adolescence, FAPα expression greatly reduced, only emerging in pathologies associated extracellular matrix remodeling. We determined whether human dental tissue involved root maturation i.e., follicle apical papilla pulp tissue. The revealed high concentration vimentin-positive cells within stromal A similar observation was made...
Abstract Extracellular vesicles (EV) are biological nanoparticles that play an important role in cell‐to‐cell communication. The phenotypic profile of EV populations is a promising reporter disease, with direct clinical diagnostic relevance. Yet, robust methods for quantifying the biomarker content have been critically lacking, and require single‐particle approach due to their inherent heterogeneous nature. Here, multicolor single‐molecule burst analysis microscopy used detect multiple...
The lipid organization of microbubbles is important in many applications.