A5036488360- Advanced Fluorescence Microscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
- Photoacoustic and Ultrasonic Imaging
- Cell Image Analysis Techniques
- Force Microscopy Techniques and Applications
- Near-Field Optical Microscopy
- Optical Coherence Tomography Applications
- Optical Imaging and Spectroscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Integrated Circuits and Semiconductor Failure Analysis
- Image Processing Techniques and Applications
- Photoreceptor and optogenetics research
- Advanced Biosensing Techniques and Applications
- DNA Repair Mechanisms
- Magnetic confinement fusion research
- Neuroscience and Neural Engineering
- Land Use and Ecosystem Services
- Nuclear Structure and Function
- Graphene and Nanomaterials Applications
- bioluminescence and chemiluminescence research
- Ionosphere and magnetosphere dynamics
- Traumatic Brain Injury and Neurovascular Disturbances
- Remote-Sensing Image Classification
- Photosynthetic Processes and Mechanisms
- RNA Interference and Gene Delivery
University of Pisa
2021-2024
Italian Institute of Technology
2013-2023
Politecnico di Milano
2021-2023
University of Lisbon
2021-2023
Institute of Photonic Sciences
2016-2019
Instituto di Biofisica
2018-2019
National Research Council
2019
National Academies of Sciences, Engineering, and Medicine
2018
University of Genoa
1994-2015
Palmetto Hematology Oncology
2000
Fluorescence recovery after photobleaching (FRAP) is a versatile tool for determining diffusion and interaction/binding properties in biological material sciences. An understanding of the mechanisms controlling requires deep structure-interaction-diffusion relationships. In cell biology, instance, this applies to movement proteins lipids plasma membrane, cytoplasm nucleus. industrial applications related pharmaceutics, foods, textiles, hygiene products cosmetics, solutes solvent molecules...
We present a method for automated spike sorting recordings with high-density, large-scale multielectrode arrays. Exploiting the dense sampling of single neurons by multiple electrodes, an efficient, low-dimensional representation detected spikes consisting estimated spatial locations and dominant shape features is exploited fast reliable clustering into units. Millions events can be sorted in minutes, parallelized scales better than quadratically number spikes. Performance demonstrated using...
Gephyrin is a key scaffold protein mediating the anchoring of GABAA receptors at inhibitory synapses. Here, we exploited superresolution techniques combined with proximity-based clustering analysis and model simulations to investigate single-molecule gephyrin reorganization during plasticity synapses in mouse hippocampal cultured neurons. This approach revealed that, expression LTP, increase density postsynaptic sites associated promoted formation nanodomains. We demonstrate that...
Abstract Functionalized carbon nano-onions (f-CNOs) are of great interest as platforms for imaging, diagnostic and therapeutic applications due to their high cellular uptake low cytotoxicity. To date, the toxicological effects f-CNOs on vertebrates have not been reported. In this study, possible biological impact zebrafish during development is investigated, evaluating different toxicity end-points such survival rate, hatching heart beat rate. Furthermore, a bio-distribution study boron...
In this article, we describe and show the application of some most advanced fluorescence superresolution techniques, STED AFM STORM microscopy towards imaging cytoskeletal structures, such as microtubule filaments. Mechanical structural properties can play a relevant role in investigation structures interest, microtubules, that provide support to cell structure. fact, mechanical properties, local stiffness elasticity, be investigated by force spectroscopy with tens nanometers resolution....
Light-sheet microscopy is a useful tool for performing biological investigations of thick samples and it has recently been demonstrated that can also act as suitable architecture super-resolution imaging by means individual molecule localization. However, in depth still limited since suffers from reduction image quality caused scattering effects. This paper sets out to investigate the advantages non-linear photoactivation implemented selective plane illumination configuration when samples....
Abstract In the replacement of genetic probes, there is increasing interest in labeling living cells with high-quality extrinsic labels, which avoid over-expression artifacts and are available a wide spectral range. This calls for broadly applicable technology that can deliver such labels unambiguously to cytosol cells. Here, we demonstrate nanoparticle-sensitized photoporation be used this end as an emerging intracellular delivery technique. We replace traditionally gold nanoparticles...
We present a new convenient method for quantitative three-dimensionally resolved diffusion measurements based on the photobleaching (FRAP) or photoactivation (FRAPa) of disk-shaped area by scanning laser beam multiphoton microscope. Contrary to previously reported spot-photobleaching protocols, this has advantage full scalability size photobleached and thus range coefficients, which can be measured conveniently. The is compatible with low as well high numerical aperture objective lenses,...
Abstract Super-Resolution Microscopy (SRM) surpasses Abbe's diffraction limit, thus enabling nanoscale observation of cells. However, SRM techniques, such as Stochastic Optical Reconstruction (STORM), suffer from long acquisition times which can significantly impact imaging throughput. To address this issue, we adapted the Enhanced Generative Adversarial Network (ESRGAN) natural to microscopy images. Our goal is generate super-resolution images widefield in shorter times. We implemented for...
In this work we report the advantages provided by two photon excitation (2PE) implemented in a selective plane illumination microscopy (SPIM) when imaging thick scattering samples. particular, detailed analysis of effects induced on real light sheet intensity distribution is performed. The comparison between single-photon and two-photon profiles shows reduction sample-induced aberrations 2PE-SPIM. Furthermore, uniformity consequent improved image contrast shown phantom samples depth These...
Abstract Stimulated emission depletion (STED) microscopy is a prominent approach of super‐resolution optical microscopy, which allows cellular imaging with so far unprecedented unlimited spatial resolution. The introduction time‐gated detection in STED significantly reduces the (instantaneous) intensity required to obtain sub‐diffraction If time‐gating combined beam operating continuous wave (CW), cheap and low labour demand implementation obtained, called gated CW‐STED microscope. However,...
We demonstrate correlative super-resolution PALM, PAINT and dSTORM imaging of RNA polymerase, membrane chromosomal DNA in fixed E. coli. This protocol allows the combination precise structural information nucleoid (dSTORM) with quantitative (PALM) interacting proteins. The spatial distribution organization polymerase are visualized bacterial cells grown at doubling times 25 or 44 min. observe that is concentrated edge highly structured during fast growth, whereas it found more evenly...
The quantitative analysis of fluorescence perturbation experiments such as recovery after photobleaching (FRAP) requires suitable analytical models to be developed. When diffusion in 3D environments is considered, the description initial condition produced by (i.e., a selected region FRAP) represents crucial aspect. Though it widely known that bleaching profiles approximations can lead errors FRAP measurements, detailed sources and effects these has never been conducted until now. In this...
Abstract In the last decade light sheet fluorescence microscopy techniques, such as selective plane illumination (SPIM), has become a well established method for developmental biology. However, conventional SPIM architectures hardly permit imaging of certain tissues since common sample mounting procedure, based on gel embedding, could interfere with morphology. this work we propose an inverted system (iSPIM), non-linear excitation, suitable 3D tissue imaging. First, iSPIM architecture...
We report thermal recovery kinetics of the lit state into parental dark state, measured for blue light-sensing photoreceptor YtvA inside overexpressing E. coli and B. subtilis bacterial cells, performed wild type several mutated proteins. Recovery was followed as a fluorescence, this property is only found but not photochemically generated state. When cells were deposited onto microscope glass plate, observed rate in photocycle ca. ten times faster comparison to purified solution. or...