A5046549738- Black Holes and Theoretical Physics
- Pulsars and Gravitational Waves Research
- Astrophysical Phenomena and Observations
- 3D Printing in Biomedical Research
- Lipid Membrane Structure and Behavior
- Quantum Electrodynamics and Casimir Effect
- Bacteriophages and microbial interactions
- Cosmology and Gravitation Theories
- Neuroscience and Neural Engineering
- Neural dynamics and brain function
- Advanced Memory and Neural Computing
- Cell Image Analysis Techniques
- Antimicrobial Peptides and Activities
- Optical Coherence Tomography Applications
- Surgical Simulation and Training
- Cellular Mechanics and Interactions
- Viral gastroenteritis research and epidemiology
- Vibrio bacteria research studies
- Retinal Imaging and Analysis
- Spectroscopy Techniques in Biomedical and Chemical Research
- Laser Material Processing Techniques
- SARS-CoV-2 detection and testing
- Optical Systems and Laser Technology
- Electron and X-Ray Spectroscopy Techniques
- Advanced Electron Microscopy Techniques and Applications
National Physical Laboratory
2016-2025
Italian Institute of Technology
2016-2018
Kingston University
2016-2017
Imperial College London
2006-2008
We present a method for automated spike sorting recordings with high-density, large-scale multielectrode arrays. Exploiting the dense sampling of single neurons by multiple electrodes, an efficient, low-dimensional representation detected spikes consisting estimated spatial locations and dominant shape features is exploited fast reliable clustering into units. Millions events can be sorted in minutes, parallelized scales better than quadratically number spikes. Performance demonstrated using...
Antimicrobial resistance stimulates the search for antimicrobial forms that may be less subject to acquired resistance. Here we report a conceptual design of protein pseudocapsids exhibiting broad spectrum activities. Unlike conventional antibiotics, these agents are effective against phenotypic bacterial variants, while clearing "superbugs" in vivo without toxicity. The adopts an icosahedral architecture is polymorphic size, but not shape, and available both l d epimeric forms. Using...
With the spread of multidrug-resistant bacteria, there has been an increasing focus on molecular classes that have not yet yielded antibiotic. A key capability for assessing and prescribing new antibacterial treatments is to compare effects agents bacterial growth at a phenotypic, single-cell level. Here, we combined time-lapse microscopy with microfluidics investigate concentration-dependent killing kinetics stationary-phase Escherichia coli cells. We used from three different classes,...
Abstract Three-dimensional cell culture systems underpin cell-based technologies ranging from tissue scaffolds for regenerative medicine to tumor models and organoids drug screening. However, realise the full potential of these requires analytical methods able capture diverse information needed characterize constituent cells, scaffold components extracellular milieu. Here we describe a multimodal imaging workflow which combines fluorescence, vibrational second harmonic generation microscopy...
Extracellular matrix type 0 is reported. The developed from a jellyfish collagen predating mammalian forms by over 0.5 billion years. With its ancient lineage, compositional simplicity, and resemblance to multiple types, the referred as extracellular 0. Here we validate describing physicochemical biological properties present it versatile, minimalist biomaterial underpinning pipeline of commercialised products under collective name JellaGelTM. We describe an extensive body evidence for...
Extracellular matrices interface with cells to promote cell growth and tissue development. Given this critical role, matrix mimetics are introduced enable biomedical materials ranging from engineering scaffolds tumor models organoids for drug screening implant surface coatings. Traditional microscopy methods used evaluate such in their ability support exploitable responses, which expressed changes proliferation rates morphology. However, the physical imaging do not capture chemistry of at...
This paper presents the concept of a technology for automation laser incisions on soft tissue, especially application in Transoral Laser Microsurgery (TLM) interventions. The aims at automatically controlling based high-level commands from surgeon, i.e. desired incision shape, length and depth. It is recently developed robotic microsurgery platform, which offers controlled motion beam surgical site. A feed-forward controller provides (i) to aiming system (ii) regulates parameters source...
We study a brane-world cosmological scenario with local inhomogeneities represented by black holes. The brane is asymmetrically embedded into the bulk. strings/cigars penetrating Friedmann generate Swiss-cheese type structure. This universe forever expands and decelerates, as its general relativistic analogue. evolution of fluid however can proceed along four branches, two allowed to have positive energy density, one them having symmetric embedding limit. On this branch future pressure...
Abstract A new method for automated spike sorting recordings with high density, large scale multielectrode arrays is presented. Exploiting the dense sampling of single neurons by multiple electrodes, we obtain an efficient, low-dimensional representation detected spikes consisting estimated spatial locations and dominant shape features, which enables fast reliable clustering into units. Millions events can be sorted in minutes, parallelized scales better than quadratically number spikes. We...
Electron microscopy plays an important role in the analysis of functional nano-to-microstructures. Substrates and staining procedures present common sources variation for analysis. However, systematic investigations on impact these data interpretation are lacking. Here we pinpoint key determinants associated with reproducibility issues imaging archetypal protein assemblies, shells, filaments. The effect morphological characteristics assemblies was assessed to reveal differential features...
The visual information processing starts in the retina.The working mechanisms of its complex stratified circuits, which ganglion cells play a central role, is still largely unknown.Understanding coding challenging and active research area also requiring automated analysis retinal images.It demands appropriate algorithms methods for studying network population strictly entangled cells.Within this framework, we propose combined technique segmenting cell (RGC) bodies, output elements method...
Viral vectors are agents enabling gene transfer and genome editing have widespread utility across the healthcare biotechnology sectors. In January 2023, International Bureau for Weights Measures’ Consultative Committee Amount of Substance (CCQM) held a workshop on Metrology systems as molecular tools. The brought together international leaders from regulatory, industry, government science, metrology sectors to better understand key challenges community: Exploring current limitations in...
a nonlinear de novo peptide topology for the assembly of synthetic virions is reported. The backbone cyclized amino-acid sequence in which polar l- and hydrophobic d-amino acid residues same-type alternate. This arrangement introduces pseudo C4 symmetries side chains within same cyclopeptide ring, allowing lateral propagation cyclopeptides into networks with [3/6, 4]-fold rotational symmetry closing virus-like shells. A combination computational experimental approaches was used to establish...
We study the evolution of a closed Friedmann brane perturbed by Hawking radiation escaping bulk black hole. The semi-transparent absorbs some infalling radiation, rest being transmitted across to other region. characterize cosmological in terms transmission rate ε. For small values ε critical-like behaviour could be observed, when acceleration due pressure and deceleration induced increasing self-gravity roughly compensate each other, is approximately same as without radiation. Lighter...
A CMOS camera for a pyramid wavefront sensor (PWS) based adaptive optics system (AOS) is proposed in this paper. The designed has four 32 x active reset pixel arrays with common addressing to produce synchronous analogue outputs. custom layout area of 12.6 sq. mm, fill factor 56% and was fabricated using Austria Micro Systems 0.35 &mgr;m C35B4 process. consumes about 56 mW power during the readout. chip enclosed box dimensions 6 cm 5.5 5 cm, which makes it suitable use an optical system....
We consider the evolution of a closed Friedmann brane irradiated by bulk black hole. Both absorption on and transmission across are allowed, latter representing generalization over previously studied model. Without transmission, critical behaviour could be observed, when acceleration due to radiation pressure deceleration introduced increasing self-gravity roughly compensate each other. show here that leads disappearance behaviour.