A5063472873- Nanoparticle-Based Drug Delivery
- Electrostatics and Colloid Interactions
- Nanoparticles: synthesis and applications
- Microfluidic and Bio-sensing Technologies
- Coagulation and Flocculation Studies
- Gold and Silver Nanoparticles Synthesis and Applications
- Polymer Surface Interaction Studies
- Field-Flow Fractionation Techniques
- Blood properties and coagulation
- Nanofabrication and Lithography Techniques
- Ion-surface interactions and analysis
- Electron and X-Ray Spectroscopy Techniques
- biodegradable polymer synthesis and properties
- Microfluidic and Capillary Electrophoresis Applications
- Nanopore and Nanochannel Transport Studies
- Advanced biosensing and bioanalysis techniques
- Protein Interaction Studies and Fluorescence Analysis
- Bone Tissue Engineering Materials
- Advanced Nanomaterials in Catalysis
- Graphene and Nanomaterials Applications
- Block Copolymer Self-Assembly
- Electrospun Nanofibers in Biomedical Applications
- Molecular Junctions and Nanostructures
- Cancer Research and Treatment
- Platelet Disorders and Treatments
National Physical Laboratory
2016-2025
Teddington Memorial Hospital
2015-2018
University of Brighton
2016
University of Sheffield
2016
Imperial College London
2008-2013
National Institute for Materials Science
2006-2010
Swiss Center for Electronics and Microtechnology (Switzerland)
2004-2006
Embryonic stem cells (ESC) are both a potential source of for tissue replacement therapies and an accessible tool to model early embryonic development. Chemical factors such as soluble growth insoluble components the extracellular matrix known affect differentiation murine ESCs. However, there is also evidence suggest that undifferentiated can sense mechanical properties their environment differentiate accordingly. By growing ESCs on flexible polydimethylsiloxane substrates with varying...
Abstract Nature has evolved mechanisms to create a diversity of specialized materials through nanoscale organization. Inspired by nature, hybrid are designed with highly tailorable properties, which achieved careful control their interactions. These novel materials, based on silica‐gelatin system, have the potential serve as platform technology for human tissue regeneration. Covalent interactions between inorganic and organic constituents essential enable precise mechanical dissolution...
The accurate characterization of submicrometer and nanometer sized particles presents a major challenge in the diverse applications envisaged for them including cosmetics, biosensors, renewable energy, electronics. Size is one principal parameters classifying understanding their behavior, with other particle characteristics usually only quantifiable when size accounted for. We present comparative study emerging established techniques to particles, evaluating sizing precision relative...
The contact of nanoparticles with biological fluids such as serum results in rapid adsorption proteins at the nanoparticle surface a layer known "protein corona". Protein coatings modify and control behavior potentially altering aggregation state cellular response, which may influence their fate hazard to human health. Cells are likely interact protein interface rather than bare surface; therefore it is important study develop appropriate measurement tools. In this we investigate how...
A new certified reference material for quality control of nanoparticle size analysis methods has been developed and produced by the Institute Reference Materials Measurements European Commission's Joint Research Centre. The material, ERM-FD102, consists an aqueous suspension a mixture silica populations distinct particle origin. characterisation relied on interlaboratory comparison study in which 30 laboratories demonstrated competence participated with variety techniques analysis. After...
Abstract We present the use of Multi-angle Dynamic Light Scattering (MADLS®) for measurement nanoparticle concentration. describe theory method and its application to nanoparticles made gold, silica polystyrene, with diameters ranging from 30 400 nm, demonstrate some limitations particles sizes 500 nm above. evaluate accuracy, linearity reproducibility, as well operational concentration size range. show that working range depends on material’s optical properties, Here it is shown level...
Comparison of ensemble (ELS) and particle-by-particle (TRPS z-PTA) based techniques to measure the zeta potential silica NPs dispersed in a complex serum matrix.
The attachment of proteins, and other biomolecules, to nanoparticles is critical interest both in the development medical products using understanding behaviour fate biological systems. Measuring amount protein attached particles a fundamental step these regards there are variety methods available for this purpose. In work, we compare use three which measure particle diameter: Dynamic Light Scattering (DLS), Nanoparticle Tracking Analysis (NTA) Differential Centrifugal Sedimentation (DCS)....
The variety of nanoparticles (NPs) used in biological applications is increasing and the study their interaction with media becoming more important. Proteins are commonly first biomolecules that NPs encounter when they interact systems either vitro or vivo. Among NPs, super-paramagnetic iron oxide (SPIONs) show great promise for medicine. In this work, we detail formation, composition, structure a monolayer bovine serum albumin (BSA) on SPIONs. We determine, both by molecular simulations...
Four common size analysis techniques were applied to engineered silica nanoparticles suspended in purified water, physiological buffer and cell culture medium, the results compared using uncertainty estimates.
Abstract The French National Metrology Institute (LNE) initiated a series of events to identify priorities for test methods and their harmonisation that directly address regulatory needs in Nanomedicine. One these workshops entitled “The International Standardisation Roadmap Nanomedicine ” held October 2023 (Paris, France) brought together key experts the characterisation nanomedicines medical products containing nanomaterials, including Joint Research Centre European Commission, SINTEF...
The precise use of nanoparticles in technological applications requires control over their surface properties. This implies the ability to quantitatively describe, for example, molecular coatings terms thickness, areal mass, or number molecules. Here, authors describe two different approaches measurement these parameters by using gold ranging diameter from 10 80 nm and coated with three proteins: immunoglobulin G, bovine serum albumin, a peptide. One approach utilizes ultraviolet-visible...
Analytical centrifugation in sedimentation and flotation modes is used to measure both the size density of polystyrene nanoparticles.
The sonication process is commonly used for de-agglomerating and dispersing nanomaterials in aqueous based media, necessary to improve homogeneity stability of the suspension. In this study, a systematic step-wise approach carried out identify optimal conditions order achieve stable dispersion. This has been adopted shown be suitable several (cerium oxide, zinc carbon nanotubes) dispersed deionized (DI) water. However, with any change either nanomaterial type or medium, there needs...
We report the results of a VAMAS (Versailles Project on Advanced Materials and Standards) inter-laboratory study measurement shell thickness chemistry nanoparticle coatings. Peptide-coated gold particles were supplied to laboratories in two forms: colloidal suspension pure water and; dried onto silicon wafer. Participants prepared analyzed these samples using either X-ray photoelectron spectroscopy (XPS) or low energy ion scattering (LEIS). Careful data analysis revealed some significant...
Measuring the number concentration of colloidal nanoparticles (NPs) is critical for assessing reproducibility, enabling compliance with regulation, and performing risk assessments NP-enabled products. For nanomedicines, their directly relates to dose. However, lack relevant reference materials established traceable measurement approaches make validation methods NP difficult. Furthermore, commercial products often exhibit agglomeration, but guidelines dealing nonideal samples are scarce. We...
We describe the outcome of a large international interlaboratory study measurement particle number concentration colloidal nanoparticles, project 10 technical working area 34, "Nanoparticle Populations" Versailles Project on Advanced Materials and Standards (VAMAS). A total 50 laboratories delivered results for 30 nm gold nanoparticles measured using tracking analysis (PTA), single inductively coupled plasma mass spectrometry (spICP-MS), ultraviolet-visible (UV-Vis) light spectroscopy,...
With a growing interest towards low batch-volume personalised medicines and continuous manufacturing of pharmaceuticals, the need for robust non-invasive quality control analytical methods is becoming increasingly important. Current for...
Micrometer-long gold nanowires were fabricated via self-assembly. Diblock copolymer films served as templates for the selective adsorption of 10 nm nanoparticles from solution to form well-defined nanostructures. An oxygen plasma treatment induced aggregation and formation continuous The electrical continuity nanostructures was observed using scanning electron microscopy.
The industrial exploitation of high value nanoparticles is in need robust measurement methods to increase the control over product manufacturing and implement quality assurance. InNanoPart, a European metrology project responded these needs by developing for particle size, concentration, agglomeration, surface chemistry shell thickness. This paper illustrates advancements this produced traceable nanoparticle number concentration liquids through small angle X-ray scattering (SAXS) single...
Polymers are attractive materials for both biomedical engineering and cardiovascular applications. Although nano-topography has been found to influence cell behaviour, no established method exists understand evaluate the effects of on polymer-blood interaction.We optimized a micro-fluidic set-up study interaction whole blood with nano-structured polymer surfaces under flow conditions. Micro-fluidic chips were coated polymethylmethacrylate films structured by demixing. Surface feature size...
The influence of fluorescence on nanoparticle size measurements using dynamic light scattering (DLS) and small angle X-ray (SAXS) was investigated.
This paper describes the production and characteristics of nanoparticle test materials prepared for common use in collaborative research project NanoChOp (Chemical optical characterization nanomaterials biological systems), casu suspensions silica nanoparticles CdSe/CdS/ZnS quantum dots (QDs). is first to illustrate how assess whether meet requirements a "reference material" (ISO Guide 30, 2015) or rather those recently defined category "representative material (RTM)" (ISO/TS 16195, 2013)....