A5016489740- RNA Interference and Gene Delivery
- Field-Flow Fractionation Techniques
- Polysaccharides and Plant Cell Walls
- Nanoparticle-Based Drug Delivery
- Advanced biosensing and bioanalysis techniques
- Advanced Cellulose Research Studies
- Viral gastroenteritis research and epidemiology
- Electrohydrodynamics and Fluid Dynamics
- Microplastics and Plastic Pollution
- Electrostatics and Colloid Interactions
- Quality and Safety in Healthcare
- Animal Virus Infections Studies
- Nanoparticles: synthesis and applications
- Biosimilars and Bioanalytical Methods
- Microbial Metabolites in Food Biotechnology
- Computational Drug Discovery Methods
- Microfluidic and Bio-sensing Technologies
- Aquaculture disease management and microbiota
- biodegradable polymer synthesis and properties
- Algal biology and biofuel production
- Extracellular vesicles in disease
- Pharmaceutical Economics and Policy
- Analytical chemistry methods development
- Coagulation and Flocculation Studies
- Recycling and Waste Management Techniques
SINTEF
2021-2024
SINTEF Industry
2024
Health Forecasting
2022
National Institute for Public Health and the Environment
2022
Material Measurement Laboratory
2020-2021
National Institute of Standards and Technology
2019-2021
Theiss Research
2020
Environnements et Paléoenvironnements Océaniques et Continentaux
2017
The availability of analytical methods for the characterization lipid nanoparticles (LNPs) in-vivo intracellular delivery nucleic acids is critical fast development innovative RNA therapies. In this study, protocols to measure (i) chemical composition, (ii) drug loading, (iii) particle size, concentration, and stability as well (iv) structure morphology were evaluated compared based on a comprehensive strategy linking key physical properties in-vitro efficacy toxicity. Furthermore,...
A comprehensive physicochemical characterization of heterogeneous nanoplastic (NPL) samples remains an analytical challenge requiring a combination orthogonal measurement techniques to improve the accuracy and robustness results. Here, batch methods, including dynamic light scattering (DLS), nanoparticle tracking analysis (NTA), tunable resistive pulse sensing (TRPS), transmission electron microscopy (TEM), scanning (SEM), as well separation/fractionation methods such centrifugal liquid...
Quality control of pharmaceutical and biopharmaceutical products, verification their safety efficacy, depends on reliable measurements critical quality attributes (CQAs). The task becomes particularly challenging for drug products vaccines containing nanomaterials, where multiple complex CQAs must be identified monitored. To reduce (i) the risk measurement bias (ii) uncertainty in decision-making during product development, combination orthogonal complementary analytical techniques are...
Liposomal formulations for the treatment of cancer and other diseases are most common form nanotechnology enabled pharmaceuticals (NEPs) submitted market approval in clinical application today. The accurate characterization their physical-chemical properties is a key requirement; particular, size, size distribution, shape, stability among that regulators identify as critical quality attributes. Here we develop validate an optimized method, based on multi-detector asymmetrical-flow field flow...
Lipid-based nanoparticles for RNA delivery (LNP-RNA) are revolutionizing the nanomedicine field, with one approved gene therapy formulation and two vaccines against COVID-19, as well multiple ongoing clinical trials. As other innovative nanopharmaceuticals (NPhs), advancement of robust methods to assess their quality safety profiles—in line regulatory needs—is critical facilitating development translation. Asymmetric-flow field-flow fractionation coupled online optical detectors (MD-AF4) is...
Abstract The French National Metrology Institute (LNE) initiated a series of events to identify priorities for test methods and their harmonisation that directly address regulatory needs in Nanomedicine. One these workshops entitled “The International Standardisation Roadmap Nanomedicine ” held October 2023 (Paris, France) brought together key experts the characterisation nanomedicines medical products containing nanomaterials, including Joint Research Centre European Commission, SINTEF...
Abstract Plastic particulates in the environment pose an increasing concern for regulatory bodies due to their potential risk higher organisms (including humans) as they enter food chain. Nanoplastics (defined here smaller than 1 μm) are particularly challenging detect and analyze at environmentally relevant concentrations biological matrices. The tunicate Ciona Robusta is effective bioindicator microplastics nanoplastic contamination marine environment, its capacity filter substantial...
Novel vaccine platforms for delivery of nucleic acids based on viral and non-viral vectors, such as recombinant adeno associated viruses (rAAV) lipid-based nanoparticles (LNPs), hold great promise. However, they pose significant manufacturing analytical challenges due to their intrinsic structural complexity. During product development process control, design, characterization, quality control require the combination fit-for-purpose complementary tools. Moreover, an in-depth methodological...
Background: This study was conducted to investigate whether mRNA vaccine technology could be adapted for the ectothermic vertebrate Atlantic salmon (Salmo salar). Lipid nanoparticle (LNP) has been developed and optimized vaccines in mammals, stabilizing facilitating its delivery into cells. However, utility at temperatures specific biological environments present ectotherms remains unclear. In addition, it is unknown if modified containing non-canonical nucleotides can correctly translate...
Background: The study was conducted to investigate if the mRNA vaccine technology could be adapted for ectothermic vertebrate Atlantic salmon (Salmo salar). lipid nanoparticle (LNP) has been developed and optimized vaccines in mammals, stabilizing facilitating delivery into cells. However, its utility at temperature specific biological environment ectotherms remains unclear. In addition, it is unknown modified containing non-canonical nucleotides can correctly translate salmonid Methods: We...
Extracellular vesicles (EVs) are traditionally divided into two major groups: (i) large originating from plasma membrane and called microvesicles, (ii) small the endoplasmic exosomes. However, it is increasingly clear that actual composition of a particular EV preparation cannot be adequately described with these simple terms much more complex. Since cell origin EVs predetermines their biological functions, understanding biogenesis important for accurate interpretation observed results. In...
Abstract The application of nanomaterials in medicine has led to novel pharmaceuticals and medical devices that have demonstrated a strong potential for increasing the efficacy/performance safety therapeutic diagnostic procedures address wide range diseases. However, successful translation these technologies from their inception (proof-of-concept) clinical practice been challenged by substantial gaps scientific technical capacity R&D companies, especially SMEs, keep up with ever-evolving...
Nanoparticles used for medical applications commonly possess coatings or surface functionalities intended to provide specific behavior in vivo, example, the use of PEG stealth properties. Direct, quantitative measurement chemistry and composition such systems a hydrated environment has thus far not been demonstrated, yet measurements are great importance development nanomedicine systems. Here we demonstrate first cryo-XPS two PEG-functionalized nanomedicines: polymeric drug delivery system...
Abstract The application of nanomaterials in medicine has led to novel pharmaceuticals and medical devices that have demonstrated a strong potential for increasing the efficacy/performance safety therapeutic diagnostic procedures address wide range diseases. However, successful translation these technologies from their inception (proof-of-concept) clinical practice been challenged by substantial gaps scientific technical capacity R&D companies, especially SMEs, keep up with ever-evolving...