The presence of two sulfur species was detected in X-ray photoelectron spectroscopy (XPS) studies thiol and disulfide molecules adsorbed onto gold surfaces. These are assigned to bound thiolate (S2p3/2 binding energy 162 eV) unbound thiol/disulfide from 163.5 164 eV). assignments consistent with XPS data obtained different thiols (C12, C16, C18, C22 alkane thiols, a fluorinated thiol, cyclic polysiloxane thiol) adsorption conditions (solvent type, concentration, temperature, rinsing). In...

XPS spectra of nine model polymers containing oxygen functional groups are studied. The measured O 1s binding energies were 532.8 eV for ether and alcohol oxgens, 532.2 ketone oxygens 533.7 the carbonyl- ether-type in ester groups, respectively. Comparison with previous experimental theoretical these is also presented. Absolute Is not agreement those published by other groups. However, exception oxygens, energy shifts observed various comparable.

Characterization of the adsorbed protein film that forms upon implantation a biomedical device is long-standing interest in biomaterials research. Time-of-flight secondary ion mass spectrometry (ToF−SIMS) powerful method for characterization proteins on biomaterial surfaces due to its chemical specificity and surface sensitivity. However, SIMS fragmentation patterns are quite complex heterogeneity sequence. Therefore, multivariate analysis technique principal components (PCA) was used obtain...

Poly(l-lysine)-g-poly(ethylene glycol) (PLL-g-PEG) copolymers with various grafting ratios were adsorbed to niobium pentoxide-coated silicon wafers and characterized before after protein adsorption using X-ray photoelectron spectroscopy (XPS) time-of-flight secondary ion mass spectrometry (ToF−SIMS). Three proteins of different sizes, myoglobin (16 kD), albumin (67 fibrinogen (340 studied. XPS was used quantify the amount bare PEGylated surfaces. ToF−SIMS principal component analysis (PCA)...

Abstract Polyethylene oxide (PEO) surfaces reduce non-specific protein and cell interactions with implanted biomaterials may improve their biocompatibility. PEO-like polymerized tetraglyme were made by glow discharge plasma deposition onto fluorinated ethylene propylene copolymer (FEP) substrates shown to adsorb less than 10 ng/cm2 of fibrinogen in vitro. The ability the resist leukocyte adhesion was studied vitro vivo. Polymerized FEP subcutaneously mice removed after 1 day or 4 weeks....

Self-assembly of thiol-terminated single-stranded DNA (HS-ssDNA) on gold has served as an important model system for immobilization at surfaces. Here, we report a detailed study the surface composition and structure mixed self-assembled monolayers containing short alkylthiol diluent [11-mercapto-1-undecanol (MCU)] supports. These were studied with X-ray photoelectron spectroscopy (XPS), near-edge absorption fine (NEXAFS), fluorescence intensity measurements. XPS results sequentially adsorbed...

Two different phosphonic acid monolayer films for immobilization of bioactive molecules such as the protein BMP-2 on titanium surfaces have been prepared. Monolayers (11-hydroxyundecyl)phosphonic and (12-carboxydodecyl)phosphonic were produced by a simple dipping process (the T-BAG method). The terminal functional groups these monolayers activated (carbonyldiimidazole hydroxyl N-hydroxysuccinimide carboxyl groups) to bind amine-containing molecules. reactivity was investigated using...

Abstract This work compares the removal of bovine aortic endothelial cell (BAEC) monolayers via 1) low‐temperature liftoff from a “smart polymer,” plasma polymerized poly( N ‐isopropyl acrylamide) (ppNIPAM), 2) enzymatic digestion, and 3) mechanical dissociation ppNIPAM surfaces. We examine surfaces after by using X‐ray photoelectron spectroscopy (XPS), time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS), immunostaining, adhesion assay. Immunoassay results indicate that...

The self-assembly of streptavidin onto biotinylated alkylthiolate monolayers on gold has served as an important model system for protein immobilization at surfaces. Here, we report a detailed study the surface composition and structure mixed self-assembled (SAMs) containing diluent alkylthiolates their use to specifically immobilize streptavidin. X-ray photoelectron spectroscopy (XPS), angle-resolved XPS (ARXPS), near-edge absorption fine (NEXAFS), plasmon resonance (SPR) have been used...

Abstract Nanomaterials advocated for biomedical applications must exhibit well‐controlled surface properties to achieve optimum performance in complex biological or physiological fluids. Dispersed materials with extremely high specific areas require as extensive characterization their macroscale biomaterials analogues. However, current literature is replete many examples of nanophase materials, most notably nanoparticles, little emphasis placed on reporting rigorous analysis...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructural Investigation of Molecular Organization in Self-Assembled Monolayers a Semifluorinated AmidethiolT. J. Lenk, V. M. Hallmark, C. L. Hoffmann, F. Rabolt, D. G. Castner, Erdelen, and H. RingsdorfCite this: Langmuir 1994, 10, 12, 4610–4617Publication Date (Print):December 1, 1994Publication History Published online1 May 2002Published inissue 1 December...

Surface-immobilized poly(N-isopropyl acrylamide) (pNIPAM) is currently used for a wide variety of biosensor and biomaterial applications. A thorough characterization the surface properties pNIPAM thin films will benefit those In this work, we present analysis plasma-polymerized NIPAM (ppNIPAM) coating by multiple analytical techniques, including time-of-flight secondary-ion mass spectrometry (ToF-SIMS), contact angle measurement, atomic force microscopy (AFM), sum frequency generation (SFG)...

We report the results of a detailed investigation chemisorption and reactivity acetylene (C2H2) ethylene (C2H4) on Rh(111) single crystal surface. Below 270 K ELS measurements indicate that chemisorbs with its C–C bond oriented parallel to surface forming an approximately sp2 hybridized species. LEED investigations show both C2H2 C2H4 form metastable (2×2) structures below K. An irreversible order–order transformation occurs between 300 stable c(4×2) hydrocarbon overlayer. The species formed...

The power of combining sum frequency generation (SFG) vibrational spectroscopy and solid-state nuclear magnetic resonance (ssNMR) to quantify, with site specificity atomic resolution, the orientation dynamics side chains in synthetic model peptides adsorbed onto polystyrene (PS) surfaces is demonstrated this study. Although isotopic labeling has long been used ssNMR studies site-specifically probe structure biomolecules, potential SFG chain isotopically labeled surface-adsorbed proteins...