A5022000597- Pluripotent Stem Cells Research
- CRISPR and Genetic Engineering
- 3D Printing in Biomedical Research
- Crystallization and Solubility Studies
- Hippo pathway signaling and YAP/TAZ
- Manufacturing Process and Optimization
- Innovative Microfluidic and Catalytic Techniques Innovation
- DNA Repair Mechanisms
- Analytical Chemistry and Chromatography
- Neuroscience and Neuropharmacology Research
- Neuroscience and Neural Engineering
- Ultrasound and Cavitation Phenomena
- Laser-Plasma Interactions and Diagnostics
- Chemical and Physical Properties in Aqueous Solutions
- Spectroscopy Techniques in Biomedical and Chemical Research
- Microfluidic and Bio-sensing Technologies
- Ion channel regulation and function
- Laser-Matter Interactions and Applications
- Engineering and Test Systems
- Metaheuristic Optimization Algorithms Research
- Forensic and Genetic Research
- Biocrusts and Microbial Ecology
- RNA Interference and Gene Delivery
- Chemical and Physical Studies
- Advanced Optical Sensing Technologies
University of Sheffield
2019-2024
Insigneo
2023-2024
Imperial College London
2015-2020
Institut Polytechnique de Bordeaux
2020
Lawrence Livermore National Laboratory
2020
First Light Fusion
2020
Rutherford Appleton Laboratory
2020
Université de Bordeaux
2020
Centre National de la Recherche Scientifique
2020
SLAC National Accelerator Laboratory
2020
The polymorphism of the simple amino acid glycine has been known for almost a century. It is also that in aqueous solutions, at isoelectric point (pI 5.9), metastable α polymorph crystallizes, while stable γ form only nucleates high and low pH. Despite importance understanding process by which crystals nucleate, solution solid-state chemistry underlying this observation have never explored. In contribution, we combined chemistry, crystallization, crystallographic data to investigate...
The appearance of genetic changes in human pluripotent stem cells (hPSCs) presents a concern for their use research and regenerative medicine. Variant hPSCs that harbor recurrent culture-acquired aneuploidies display growth advantages over wild-type diploid cells, but the mechanisms yield drift from predominantly to variant cell populations remain poorly understood. Here, we show dominance clones mosaic cultures is enhanced through competitive interactions result elimination cells. This...
Culture-acquired variants in human pluripotent stem cells (hPSCs) hinder their applications research and clinic. However, the mechanisms that underpin selection of remain unclear. Here, through analysis comprehensive karyotyping datasets from over 23,000 hPSC cultures more than 1,500 lines, we explored how culture conditions shape variant selection. Strikingly, identified an association chromosome 1q gains with feeder-free noted a rise its prevalence recent years, coinciding increased usage...
Sonochemistry, or the application of power ultrasound to chemical processes, is a technology that has undergone very intensive research and development in last 15−20 years, with respect both its range methods employed for implementation at industrial manufacturing scales. In series two papers, authors review progress chemistry processing, equipment scale-up methods. This first paper focuses on also covers mechanisms action situ detection measurement. Part 2 will deal crystallization...
Limb girdle muscular dystrophy type 2L or anoctaminopathy is a condition mainly characterized by adult onset proximal lower limb weakness and raised CK values, due to recessive ANO5 gene mutations. An exon 5 founder mutation (c.191dupA) has been identified in most of the British German LGMD2L patients so far reported. We aimed further investigate prevalence spectrum mutations related clinical phenotypes, screening 205 undiagnosed referred our molecular service with suspicion anoctaminopathy....
Human pluripotent stem cells (PSCs) are subject to the appearance of recurrent genetic variants on prolonged culture. We have now found that, compared with isogenic differentiated cells, PSCs exhibit evidence considerably more DNA damage during S phase cell cycle, apparently as a consequence replication stress marked by slower progression replication, activation latent origins and collapse forks. As in many cancers, which, like PSCs, shortened G1 stress, resulting may underlie higher...
Summary Since the first derivation of human pluripotent stem cells (hPSCs), number culture conditions has steadily increased, making hPSC more facile. Nonetheless, there remains persistent issue culture-acquired genetic changes, hampering reproducibility research and jeopardising their clinical use. Here, we utilised comprehensive karyotyping datasets from over 20,000 cultures sampled under different to ascertain association changes with specific regimens. We found condition-dependent...
We report on the design, construction, and characterisation of a new class in-vacuo optical levitation trap optimised for use in high-intensity, high-energy laser interaction experiments. The system uses focused, vertically propagating continuous wave beam to capture manipulate micro-targets by photon momentum transfer at much longer working distances than commonly used tweezer systems. A high speed (10 kHz) imaging signal acquisition was implemented tracking levitated droplets position...
Abstract We postulate that exit from pluripotency involves intermediates retain while simultaneously exhibiting lineage-bias. Using a MIXL1 reporter, we explore mesoderm lineage-bias within the human pluripotent stem cell compartment. identify substate, which at single level coexpresses and mesodermal gene expression programmes. Functionally these cells initiate cultures exhibit bias in differentiation assays. By promoting identity through manipulation of WNT signalling preventing using...
A measurement-based closed-loop control system using in-process ATR-FTIR spectroscopy coupled with a multivariate chemometric PLS calibration model is developed, validated, and applied to the monitoring of supersaturation in 250- L industrial pilot-plant crystalliser. Supersaturation experiments are carried out on seeded batch cooling crystallisation β-l-glutamic acid from aqueous solutions two methods seeding involving addition seeds solution generation within solution. The generic...
Extracellular matrices interface with cells to promote cell growth and tissue development. Given this critical role, matrix mimetics are introduced enable biomedical materials ranging from engineering scaffolds tumor models organoids for drug screening implant surface coatings. Traditional microscopy methods used evaluate such in their ability support exploitable responses, which expressed changes proliferation rates morphology. However, the physical imaging do not capture chemistry of at...
Background aimsThe appearance of genetically variant populations in human pluripotent stem cell (hPSC) cultures represents a concern for research and clinical applications. Genetic variations may alter hPSC differentiation potential or cause phenotype variation differentiated cells. Further, variants have properties such as proliferative rate, response to the culture environment, that differ from wild-type As such, understanding behavior these culture, any operational impact on manufacturing...
The automated sneak analysis software described in this paper works well on classic examples. It correctly identifies paths without falsely reporting nonexistent sneaks. has been tried out much larger real-world examples, and reproduces the kind of behavior that it had demonstrated Future development tool will enable exploration internal states complex components such as ECUs. In addition provision to complete a presence failures further enhance tool. use simulation identification...
We report on the design and testing of a multiwavelength interferometry system for Orion laser facility based upon use self-path matching Wollaston prisms. The UV corrected achromatic optics allows both easy alignment with an eye-safe light source small (∼ millimeter) offsets to focal lengths between different operational wavelengths. Interferograms are demonstrated at wavelengths corresponding first, second, fourth harmonics 1054 nm Nd:glass probe beam. Example data confirms broadband...
Abstract Human pluripotent stem cell (hPSC)-derived cardiomyocytes have emerged as powerful tools for disease modelling and therapy. The production of from hPSCs typically requires expanding large numbers maintaining them in culture extended periods time. This turn predisposes to the acquisition non-random genetic changes, including recurrent gains chromosome 1q. Here, we show that gain 1q affects both efficiency differentiation phenotype differentiated cells. Mechanistically, aberrant...
Abstract Cell‐cell interactions are required for development and homeostasis in multicellular organisms from insects to mammals. A critical process governed by these is cell competition, which functions throughout control tissue composition eliminating cells that possess a lower fitness status than their neighbors. Human pluripotent stem (hPSCs) key biological tool modeling human offer further potential as source of clinically relevant populations regenerative medicine applications....
Abstract The appearance of genetic changes in human pluripotent stem cells (hPSCs) presents a concern for their use research and regenerative medicine. Variant hPSCs harbouring recurrent culture-acquired aneuploidies display growth advantages over wild-type diploid cells, but the mechanisms yielding drift from predominantly to variant cell populations remain poorly understood. Here we show that dominance clones mosaic cultures is enhanced through competitive interactions resulting...
Radiative-shocks induced by laser–cluster interactions are modeled using radiation-hydrodynamic simulations. A good agreement—in both shock velocity and density profiles—is obtained between experiment simulations, indicating that non-local thermodynamic equilibrium (NLTE) radiative effects important in the experimental regime examined, particularly at early times (≤30 ns) due to elevated temperatures (≥35 eV). The enhanced NLTE emission causes be reduced amplitude, increased width,...
Human pluripotent stem cells (PSC) often acquire genetic changes on prolonged culture, which pose concerns for their use in research and regenerative medicine (Amps et al., 2011, Seth 2011). The acquisition of these during culture necessarily first requires mutation then selection those mutations that provide a growth advantage. Whilst accounts the recurrent nature variants commonly reported (Draper 2004, Olariu 2010), mechanisms PSC remain largely elusive. Here we show that, contrast to...
Creating diagnostic information for complex technical systems has become a very costly and challenging process. Separation of the process creating diagnostics from processes design reliability analysis (as is default in many companies) means that work often ignored or repeated by engineers diagnostics. The current paper explains basic concepts behind creation systems, considers extent to which results can be used make production more efficient. In particular, it how automated analyses fed...