A5019658591- 3D Printing in Biomedical Research
- Pluripotent Stem Cells Research
- Liver physiology and pathology
- Animal testing and alternatives
- CRISPR and Genetic Engineering
- Monoclonal and Polyclonal Antibodies Research
- Diabetes Treatment and Management
- Analytical Chemistry and Chromatography
- Mesenchymal stem cell research
- RNA Interference and Gene Delivery
- Lipid Membrane Structure and Behavior
- Drug Solubulity and Delivery Systems
- Inhalation and Respiratory Drug Delivery
- Mathematical Biology Tumor Growth
- Cancer Research and Treatments
- Diabetes Management and Research
- Hydrogels: synthesis, properties, applications
- Mitochondrial Function and Pathology
- Single-cell and spatial transcriptomics
- Skin Protection and Aging
- Periodontal Regeneration and Treatments
- Tissue Engineering and Regenerative Medicine
- Hemoglobin structure and function
- Ubiquitin and proteasome pathways
- Pickering emulsions and particle stabilization
University of Sheffield
2018-2024
The Royal Melbourne Hospital
2020
Mitochondria are unavoidably subject to organellar stress resulting from exposure a range of reactive molecular species. Consequently, cells operate poorly understood quality control programme mitophagy facilitate elimination dysfunctional mitochondria. Here, we used model stressor, deferiprone (DFP), investigate the basis for stress-induced mitophagy. We show that mitochondrial fission 1 protein (Fis1) is required DFP-induced and Fis1 SUMOylated at K149, an amino acid residue critical...
Culture-acquired variants in human pluripotent stem cells (hPSCs) hinder their applications research and clinic. However, the mechanisms that underpin selection of remain unclear. Here, through analysis comprehensive karyotyping datasets from over 23,000 hPSC cultures more than 1,500 lines, we explored how culture conditions shape variant selection. Strikingly, identified an association chromosome 1q gains with feeder-free noted a rise its prevalence recent years, coinciding increased usage...
In early preclinical drug development, potential candidates are tested in the laboratory using isolated cells. These vitro experiments traditionally involve cells cultured a two-dimensional monolayer environment. However, three-dimensional spheroid systems have been shown to more closely resemble functionality and morphology of vivo . While increasing usage hepatic cultures allows for relevant experimentation realistic biological environment, underlying physical processes transport, uptake...
Many in vitro liver cell models, such as 2D systems, that are used to assess the hepatotoxic potential of xenobiotics suffer major limitations arising from a lack preservation physiological phenotype and metabolic competence. To circumvent some these there has been increased focus on producing more representative 3D models. Here we have novel approach construct size-controllable hepatic spheroid model using freshly isolated primary rat hepatocytes (PRH) utilising liquid-overlay technique...
Summary Since the first derivation of human pluripotent stem cells (hPSCs), number culture conditions has steadily increased, making hPSC more facile. Nonetheless, there remains persistent issue culture-acquired genetic changes, hampering reproducibility research and jeopardising their clinical use. Here, we utilised comprehensive karyotyping datasets from over 20,000 cultures sampled under different to ascertain association changes with specific regimens. We found condition-dependent...
Aims and experimental design. The acute-phase protein haptoglobin (Hp) has been recently detected in colorectal cancer (CRC) tissue, where its expression correlates with metastasis. Recently, we identified Hp as a CDw75 antigen-expressing tissue. To deepen the knowledge of this CRC, studied healthy tumour tissue specimens from 62 CRC patients by immunohistochemistry Western blotting, well Caco-2 HT-29 cell lines quantitative PCR, immunofluorescence microscopy flow cytometry. Results...
There are no physical or visual manifestations that define skin sensitivity irritation; a subjective diagnosis is made on the basis of evaluation clinical presentations, including burning, prickling, erythema, and itching. Adverse reaction in response to topically applied products common can limit use dermatological cosmetic products. The purpose this study was evaluate human equivalents based immortalized keratinocytes potential 22-gene panel combination with multivariate analysis...
In pharmaceutical therapeutic design or toxicology, accurately predicting the permeation of chemicals through human epithelial tissues is crucial, where significantly influenced by tissue's cellular architecture. Current mathematical models for multi-layered epithelium such as oral mucosa only use simplistic 'bricks and mortar' geometries therefore do not account complex architecture these at microscale level, extensive plasma membrane convolutions that define extracellular spaces between...
Porous, biodegradable polycaprolactone microspheres support mesenchymal progenitor cell growth and differentiation. Only cells inside the differentiate into an osteocyte-like phenotype, indicating role of physical environmental cues.
Skin irritancy to topically applied chemicals is a significant problem that affects millions of people worldwide. New or modified chemical entities must be tested for potential skin by industry as part the safety and toxicity profiling process. Many these tests have now moved non-animal-based format reduce experiments on animals. However, often rely monolayer cultures keratinocytes are not representative architecture tissue-engineered human equivalents (HSE) using complex multi-gene...
Abstract Aim To determine the clinical and biochemical variables associated with change in HbA1c patients type 2 diabetes who start sodium‐glucose linked transporter (SGLT) inhibitor therapy. Methods We performed a prospective cohort study (ACTRN12616000833460) of 48 adults (30 male, 18 female) attended tertiary hospital clinic. Fasting serum urine samples, collected during clinic visits prior to at 1, 12 24 weeks after commencing SGLT treatment, were analysed for HbA1c, electrolytes, urea,...