A5024275136- Mesenchymal stem cell research
- Nerve injury and regeneration
- Mast cells and histamine
- Spinal Cord Injury Research
- Hereditary Neurological Disorders
- Periodontal Regeneration and Treatments
- Immune cells in cancer
- Tissue Engineering and Regenerative Medicine
- Endoplasmic Reticulum Stress and Disease
- Autophagy in Disease and Therapy
- Pluripotent Stem Cells Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Oral and Maxillofacial Pathology
- dental development and anomalies
- Electrospun Nanofibers in Biomedical Applications
- Neurogenesis and neuroplasticity mechanisms
- Peptidase Inhibition and Analysis
- Immune Cell Function and Interaction
- Phagocytosis and Immune Regulation
- Immune Response and Inflammation
- Extracellular vesicles in disease
- Peripheral Neuropathies and Disorders
- Neurological diseases and metabolism
- Pregnancy-related medical research
- Adenosine and Purinergic Signaling
Hasselt University
2016-2025
VIB-KU Leuven Center for Brain & Disease Research
2022-2025
Biomedical Research Institute
2016-2024
KU Leuven
2022-2023
Morpho (United States)
2016
John Wiley & Sons (United States)
2016
The therapeutic effects of mesenchymal stem cell (MSC) transplantation following spinal cord injury (SCI) to date have been limited. Therefore, we aimed enhance the immunomodulatory properties MSCs via continuous secretion anti-inflammatory cytokine interleukin-13 (IL-13). By using as carriers IL-13 (MSC/IL-13), investigated their potential, compared with non-engineered MSCs, in a mouse model SCI. We show that transplanted MSC/IL-13 significantly improve functional recovery SCI, and also...
Abstract Leukocyte- and Platelet-Rich Fibrin (L-PRF) is an autologous platelet concentrate, consisting of a fibrin matrix enriched with platelets, leukocytes plethora cytokines growth factors. Since L-PRF produced bedside from whole blood without the use anti-coagulant, it becoming popular adjuvant in regenerative medicine. While other types concentrates have been described to stimulate vessel formation, little known about angiogenic capacities L-PRF. Therefore, this study aimed fully...
Foamy macrophages containing abundant intracellular myelin remnants are an important pathological hallmark of multiple sclerosis. Reducing the lipid burden in foamy is considered a promising therapeutic strategy to induce phagocyte phenotype that promotes central nervous system repair. Recent research from our group showed sustained accumulation myelin-derived lipids skews these phagocytes toward disease-promoting and more inflammatory phenotype. Our data now demonstrate disturbed lipophagy,...
Mast cells (MCs) are found abundantly in the central nervous system and play a complex role neuroinflammatory diseases such as multiple sclerosis stroke. In present study, we show that MC-deficient Kit(W-sh/W-sh) mice display significantly increased astrogliosis T cell infiltration well reduced functional recovery after spinal cord injury compared to wildtype mice. addition, levels of MCP-1, TNF-α, IL-10 IL-13 protein cord. Mice deficient mouse mast protease 4 (mMCP4), an MC-specific...
Abstract Charcot–Marie–Tooth disease type 1A (CMT1A) is the most common inherited peripheral neuropathy caused by a 1.5 Mb tandem duplication of chromosome 17 harbouring PMP22 gene. This dose-dependent overexpression results in disrupted Schwann cell myelination nerves. To obtain better insights into underlying pathogenic mechanisms CMT1A, we investigated role cellular homeostasis CMT1A mouse models and patient-derived induced pluripotent stem cells differentiated precursors (iPSC-SCPs). We...
Abstract Macrophages play major roles in the pathophysiology of various neurological disorders, being involved seemingly opposing processes such as lesion progression and resolution. Yet, molecular mechanisms that drive their harmful benign effector functions remain poorly understood. Here, we demonstrate extracellular vesicles (EVs) secreted by repair‐associated macrophages (RAMs) enhance remyelination ex vivo promoting differentiation oligodendrocyte precursor cells (OPCs). Guided...
An important barrier for axon regeneration and recovery after traumatic spinal cord injury (SCI) is attributed to the scar that formed at lesion site. Here, we investigated effect of mouse mast cell protease (mMCP) 6, a (MC)-specific tryptase, on scarring functional hemisection injury. Functional was significantly impaired in both MC-deficient mMCP6-knockout (mMCP62/2) mice SCI compared with wild-type control mice. This decrease locomotor performance associated an increased size excessive...
A disintegrin and metalloprotease 17 (ADAM17) is a sheddase with important substrates including tumor necrosis factor-α (TNF-α) its receptors, the p75 neurotrophin receptor (p75NTR), members of epidermal growth factor family. The rationale this study was to inhibit ADAM17-induced shedding soluble TNF-α in order reduce detrimental inflammation after spinal cord injury (SCI). However, using specific ADAM17 blocker BMS-561392 neuronal glial cell cultures, we show that proper functioning vital...
Neurological disorders are characterized by neurodegeneration and/or loss of neuronal function, which cannot be adequately repaired the host. Therefore, there is need for novel treatment options such as cell-based therapies that aim to salvage or reconstitute lost tissue stimulate host repair. The present study aimed evaluate paracrine effects human dental pulp stem cells (hDPSCs) on migration and neural maturation SH-SY5Y neuroblastoma cells. hDPSC secretome had a significant...
Abstract Traumatic spinal cord injury (SCI) most often leads to permanent paralysis due the inability of axons regenerate in adult mammalian central nervous system (CNS). In past, we have shown that mast cells (MCs) improve functional outcome after SCI by suppressing scar tissue formation at lesion site via mouse cell protease 6 (mMCP6). this study, investigated whether recombinant mMCP6 can be used therapeutically SCI. Therefore, applied locally an intrathecal catheter subacute phase a...
Abstract Spinal cord injury (SCI) triggers the formation of a glial and fibrotic scar, which creates major barrier for neuroregenerative processes. Previous findings indicate that mast cells (MCs) protect spinal after mechanical damage by suppressing detrimental inflammatory processes via mouse cell protease 4 (mMCP4), MC-specific chymase. In addition to these immunomodulatory properties, mMCP4 also plays an important role in tissue remodeling extracellular matrix degradation. Therefore, we...
Fibroblast activation protein-α (FAPα) is a membrane protein with dipeptidyl-peptidase and type I collagenase activity expressed during fetal growth. At the age of adolescence, FAPα expression greatly reduced, only emerging in pathologies associated extracellular matrix remodeling. We determined whether human dental tissue involved root maturation i.e., follicle apical papilla pulp tissue. The revealed high concentration vimentin-positive cells within stromal A similar observation was made...
Background: Dysregulation of the endo-lysosomal–autophagy pathway has been identified as a critical factor in pathology various demyelinating neurodegenerative diseases, including peripheral neuropathies. This plays crucial role transporting newly synthesized myelin proteins to plasma membrane myelinating Schwann cells, making these cells susceptible lysosome-related dysfunctions. Nevertheless, specific impact lysosomal dysfunction and its contribution neurodegeneration remain poorly...
Autologous plasma fractions, such as platelet-rich (PRP) and platelet-poor (PPP), contain growth factors that can enhance neural cell survival are therefore likely to have the ability promote nerve regeneration. The present study compared effect of PRP PPP application on myelinated density diameter in peri-implant bone region. In addition, healing time regeneration was assessed. Nine beagle dogs randomly received 54 dental implants bilateral mandible according a split-mouth design. Each...
Pathologies of the central nervous system are characterized by loss brain tissue and neuronal function which cannot be adequately restored endogenous repair processes. This stresses need for novel treatment options such as cell-based therapies that able to restore damaged or stimulate repair. study investigated neuroregenerative potential conditioned medium human dental pulp stem cells (CM-hDPSCs) on neural cell (NSC) proliferation migration well neurite outgrowth primary cortical neurons...