A5000183150- Spinal Cord Injury Research
- Nerve injury and regeneration
- Neuroinflammation and Neurodegeneration Mechanisms
- Mast cells and histamine
- Neurogenesis and neuroplasticity mechanisms
- Immune cells in cancer
- Mesenchymal stem cell research
- Immune Response and Inflammation
- Stroke Rehabilitation and Recovery
- thermodynamics and calorimetric analyses
- Spinal Dysraphism and Malformations
- Immune Cell Function and Interaction
- Traumatic Brain Injury and Neurovascular Disturbances
- Wound Healing and Treatments
- Tissue Engineering and Regenerative Medicine
- Olfactory and Sensory Function Studies
- Adenosine and Purinergic Signaling
- 3D Printing in Biomedical Research
- Eosinophilic Disorders and Syndromes
- Graphene and Nanomaterials Applications
- Anesthesia and Neurotoxicity Research
- IL-33, ST2, and ILC Pathways
- Photoreceptor and optogenetics research
- Spinal Hematomas and Complications
- Pregnancy-related medical research
University College Dublin
2017-2023
Trinity College Dublin
2017-2018
Hasselt University
2012-2016
University of Antwerp
2016
Abstract Spinal cord injury (SCI) is a devastating condition with no curative therapy currently available. Immunomodulation can be applied as therapeutic strategy to drive alternative immune cell activation and promote proregenerative microenvironment. Locally injected hydrogels carrying immunotherapeutic cargo directly injured tissue offer an encouraging treatment approach from immunopharmacological perspective. Gelatin methacrylate (GelMA) are promising in this regard, however, detailed...
The therapeutic effects of mesenchymal stem cell (MSC) transplantation following spinal cord injury (SCI) to date have been limited. Therefore, we aimed enhance the immunomodulatory properties MSCs via continuous secretion anti-inflammatory cytokine interleukin-13 (IL-13). By using as carriers IL-13 (MSC/IL-13), investigated their potential, compared with non-engineered MSCs, in a mouse model SCI. We show that transplanted MSC/IL-13 significantly improve functional recovery SCI, and also...
Mast cells (MCs) are found abundantly in the central nervous system and play a complex role neuroinflammatory diseases such as multiple sclerosis stroke. In present study, we show that MC-deficient Kit(W-sh/W-sh) mice display significantly increased astrogliosis T cell infiltration well reduced functional recovery after spinal cord injury compared to wildtype mice. addition, levels of MCP-1, TNF-α, IL-10 IL-13 protein cord. Mice deficient mouse mast protease 4 (mMCP4), an MC-specific...
Promoting the neuroprotective and repair-inducing effector functions of microglia macrophages, by means M2 polarisation or alternative activation, is expected to become a new therapeutic approach for central nervous system (CNS) disorders in which detrimental pro-inflammatory and/or macrophages display major contribution neuropathology. In this study, we present novel vivo using intracerebral grafting mesenchymal stem cells (MSC) genetically engineered secrete interleukin 13 (IL13-MSC).In...
An important barrier for axon regeneration and recovery after traumatic spinal cord injury (SCI) is attributed to the scar that formed at lesion site. Here, we investigated effect of mouse mast cell protease (mMCP) 6, a (MC)-specific tryptase, on scarring functional hemisection injury. Functional was significantly impaired in both MC-deficient mMCP6-knockout (mMCP62/2) mice SCI compared with wild-type control mice. This decrease locomotor performance associated an increased size excessive...
A disintegrin and metalloproteinase 17 (ADAM17) is the major sheddase involved in cleavage of a plethora cytokines, cytokine receptors growth factors, thereby playing substantial role inflammatory regenerative processes after central nervous system trauma. By making use hypomorphic ADAM17 knockin mouse model as well pharmacological ADAM10/ADAM17 inhibitors, we showed that ADAM17-deficiency or inhibition significantly increases clearance apoptotic cells, promotes axon improves functional...
Innate lymphocyte populations, such as innate lymphoid cells (ILCs), γδ T cells, invariant natural killer (iNK T) and mucosal-associated (MAIT) are emerging important effectors of immunity involved in various inflammatory autoimmune diseases. The aim this study was to assess the frequencies absolute numbers lymphocytes well conventional monocytes peripheral blood from a cohort anti-neutrophil cytoplasm autoantibody (ANCA)-associated vasculitis (AAV) patients. Thirty-eight AAV patients 24...
Abstract Transplantation of mesenchymal stem cells (MSCs) into injured or diseased tissue—for the in situ delivery a wide variety MSC-secreted therapeutic proteins—is an emerging approach for modulation clinical course several diseases and traumata. From emergency point-of-view, allogeneic MSCs have numerous advantages over patient-specific autologous since “off-the-shelf” cell preparations could be readily available instant intervention following acute injury. Although we confirmed vitro...
Animal models with constant, long-lasting motor deficits together the right tests to assess behavioral abnormalities are needed study effectiveness of potential therapies restore functions. In current study, controlled cortical impact (CCI) was applied in rats induce damage forelimb area cortex and dorsal striatum. Motor behavior assessed before after CCI, using fine skill such as adhesive removal test, cylinder Montoya staircase test well automated gait analysis system CatWalk XT over a 6...
Spinal cord injury (SCI) elicits a robust neuroinflammatory reaction which, in turn, exacerbates the initial mechanical damage. Pivotal players orchestrating this response are macrophages (Mφs) and microglia. After SCI, inflammatory environment is dominated by pro-inflammatory Mφs/microglia, which contribute to secondary cell death prevent regeneration. Therefore, reprogramming Mφ/microglia towards more anti-inflammatory potentially neuroprotective phenotype has gained substantial...
Abstract Traumatic spinal cord injury (SCI) most often leads to permanent paralysis due the inability of axons regenerate in adult mammalian central nervous system (CNS). In past, we have shown that mast cells (MCs) improve functional outcome after SCI by suppressing scar tissue formation at lesion site via mouse cell protease 6 (mMCP6). this study, investigated whether recombinant mMCP6 can be used therapeutically SCI. Therefore, applied locally an intrathecal catheter subacute phase a...
After spinal cord injury (SCI), monocyte derived macrophages play a detrimental role.Histone deacetylases (HDACs) are central epigenetic regulators of macrophage-polarization.We hypothesized that HDAC3 inhibition suppresses the pro-inflammatory macrophage phenotype (M1), promotes anti-inflammatory (M2) and improves functional recovery after SCI.Therefore, two inhibitors were selected, namely scriptaid RGFP966.The impact on polarization was studied by investigating effect gene protein...
Abstract Spinal cord injury (SCI) triggers the formation of a glial and fibrotic scar, which creates major barrier for neuroregenerative processes. Previous findings indicate that mast cells (MCs) protect spinal after mechanical damage by suppressing detrimental inflammatory processes via mouse cell protease 4 (mMCP4), MC-specific chymase. In addition to these immunomodulatory properties, mMCP4 also plays an important role in tissue remodeling extracellular matrix degradation. Therefore, we...
Traumatic spinal cord injuries result from high impact forces acting on the spine and are proceeded by an extensive secondary inflammatory response resulting in motor, sensory, autonomic dysfunction. Experimental vivo traumatic rodents using a contusion model have been extremely useful elucidating underlying pathophysiology of these injuries. However, relationship between biomechanical factors is still not well understood. Therefore, aim this research to provide comprehensive analysis...
The cytokine, interleukin (IL)-25, is thought to be critically involved in inducing a type 2 immune response which may contribute regeneration after central nervous system (CNS) trauma. We investigated whether applying recombinant IL-25, locally or systemically, mouse model of spinal cord injury (SCI) improves functional and histological recovery.Repeated systemic administration IL-25 did not influence recovery following SCI. In contrast, single local significantly worsened locomotor...
Live-cell imaging is a common technique in microscopy to investigate dynamic cellular behaviour and permits the accurate relevant analysis of wide range tissue parameters, such as motility, cell division, wound healing responses calcium (Ca 2 + ) signalling lines, primary cultures ex vivo preparations. Furthermore, this can occur under many experimental conditions, making live-cell indispensable for biological research. Systems which maintain cells at physiological conditions outside CO...
A mounting body of evidence suggests that stress plays a major role in the injury progression after spinal cord (SCI). Injury activates systems; this turn may augment generation pro-inflammatory cytokines, stimulate immune cells, and alter balance between pro- anti-inflammatory response. As result, it is suggested pathways neuronal damage loss SCI. Considering these potential detrimental effects SCI, we hypothesized inhibition immediately SCI offer protection from improve recovery. To...